Molecular mapping of stripe rust resistance gene YrHu derived from Psathyrostachys huashanica

F., D.; Fang, Zheng Wu; Yin, Junliang; Chao, Kaixiang; Jing, Jinxue; Li, Q.; Wang, B. T.

doi:10.1007/s11032-016-0487-6

Cited by 28 publications

(21 citation statements)

References 21 publications

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“…Seeds of Emai 170 (a hexaploid common wheat variety) were surface sterilized with 1% hydrogen peroxide, thoroughly rinsed with distilled water, and germinated for 2 days in a 20 ° C incubator (Ma et al, 2016). According to the reported method, the seedlings were transferred and cultured in a continuously ventilated 1/4 strength Hoagland nutrient solution (Zhu et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum)

Jiang

Yang

et al. 2019

PeerJ

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Superoxide dismutases (SODs) are a family of key antioxidant enzymes that play a crucial role in plant growth and development. Previously, this gene family has been investigated in Arabidopsis and rice. In the present study, a genome-wide analysis of the SOD gene family in wheat were performed. Twenty-six SOD genes were identified from the whole genome of wheat, including 17 Cu/Zn-SODs, six Fe-SODs, and three Mn-SODs. The chromosomal location mapping analysis indicated that these three types of SOD genes were only distributed on 2, 4, and 7 chromosomes, respectively. Phylogenetic analyses of wheat SODs and several other species revealed that these SOD proteins can be assigned to two major categories. SOD1 mainly comprises of Cu/Zn-SODs, and SOD2 mainly comprises of Fe-SODs and Mn-SODs. Gene structure and motif analyses indicated that most of the SOD genes showed a relatively conserved exon/intron arrangement and motif composition. Analyses of transcriptional data indicated that most of the wheat SOD genes were expressed in almost all of the examined tissues and had important functions in abiotic stress resistance. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to reveal the regulating roles of wheat SOD gene family in response to NaCl, mannitol, and polyethylene glycol stresses. qRT-PCR showed that eight randomly selected genes with relatively high expression levels responded to all three stresses based on released transcriptome data. However, their degree of response and response patterns were different. Interestingly, among these genes, TaSOD1.7, TaSOD1.9, TaSOD2.1, and TaSOD2.3 feature research value owing to their remarkable expression-fold change in leaves or roots under different stresses. Overall, our results provide a basis of further functional research on the SOD gene family in wheat and facilitate their potential use for applications in the genetic improvement on wheat in drought and salt stress environments.

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Section: Methodsmentioning

confidence: 99%

Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum)

Jiang

Yang

et al. 2019

PeerJ

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“…At present, four stripe rust resistance genes from wheat– P . huashanica translocation lines have been identified by our group (Cao et al., 2008; Li et al., 2012; Ma et al., 2013, 2016, 2019; Sun et al., 2019). These genes possess high level of resistance to the tested Pst races.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, several excellent resistance genes to most Chinese Pst races were found from P. huashanica . The resultant genes were temporarily named YrH9015 (Sun et al., 2019), YrH921 (Ma et al., 2019), YrHu (Ma et al., 2016), YrHA (Ma et al., 2013), YrH9020 (Li et al., 2012), YrHy (Yao et al., 2010) and YrHua (Cao et al ., 2008). In this study, the wheat translocation line H921‐11‐1 possesses resistance to all Chinese Pst races from 1990 to the present.…”

Section: Introductionmentioning

confidence: 99%

Molecular mapping of stripe rust resistance gene YrH921 in a wheat introgression line H921‐11‐1

Jiang

Sun

et al. 2020

Journal of Phytopathology

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Several novel stripe rust pathogen races emerging in the wheat‐planting regions of China in recent years were virulent to a majority of the designated wheat seedling resistance genes. Therefore, it is of great significance to continuously select more new and valuable materials for enriching resistant sources diversity, pyramiding different excellent resistance genes and achieving durable resistance. In this study, a resistance gene, temporarily designated as YrH921, was identified from wheat–Psathyrostachys huashanica introgression line H921‐11‐1. Two hybrid populations, 160 F2 plants and corresponding derived F2:3 families, of the two parents about Mingxian169 as female and H921‐11‐1 as male, were used to evaluate stripe rust resistance in seedling stage and as a mapping population. At last, a genetic map which comprises of four simple sequence repeat (SSR) markers and six expressed sequence tag (EST) markers was constructed. YrH921 was located on the long arm of chromosome 5A. Two closely linked EST‐STS markers (BF483937 and BF484913) were screened, and the genetic distance linked to YrH921 was 3.0 and 4.3 cM, respectively. There was great value in research and production if the two closest markers were effectively used for marker‐assisted selection of YrH921 in breeding.

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“…Young healthy leaves from R39, Mingxian 169 and F 2 individuals were collected, frozen in liquid nitrogen, and used for DNA extraction. Total genomic DNA was prepared from each plant using the CTAB method 5 . The DNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Breeding and growing resistant cultivars is the most economic, effective and environmentally friendly approach to control Pst 5 . Expression of rust resistance is usually described as all-stage resistance (ASR) effective in seedlings as well as adult plants, and adult-plant resistance (APR) 6 .…”

Section: Introductionmentioning

confidence: 99%

Rapid identification of a stripe rust resistant gene in a space-induced wheat mutant using specific locus amplified fragment (SLAF) sequencing

Yin

Fang

Sun

et al. 2018

Sci Rep

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Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat. Resistant cultivars are the preferred strategy to control the disease. Space-induced wheat mutant R39 has adult-plant resistance (APR) to Pst. Genetic analysis indicated that a single recessive gene, designated YrR39, was responsible for the APR of R39 to Pst. Bulked segregant analysis (BSA) combined with a SLAF sequencing (SLAF-seq) strategy was used to fine-map YrR39 to a 17.39 Mb segment on chromosome 4B. The region was confirmed by analysis with simple sequence repeat (SSR) markers. A total of 126 genes were annotated in the region and 21 genes with annotations associated with disease response were selected for further qRT-PCR analysis. The candidate gene Traes_4BS_C868349E1 (annotated as an F-box/LRR-repeat protein) was up-regulated after 12, 24, 48, and 96 hours post inoculation with Pst, suggesting it is likely involved in the resistance. The current study demonstrated that BSA combined with SLAF-seq for SNP discovery is an efficient approach for mapping and identifying candidate functional gene.

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Molecular mapping of stripe rust resistance gene YrHu derived from Psathyrostachys huashanica

Cited by 28 publications

References 21 publications

Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum)

Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum)

Molecular mapping of stripe rust resistance gene YrH921 in a wheat introgression line H921‐11‐1

Rapid identification of a stripe rust resistant gene in a space-induced wheat mutant using specific locus amplified fragment (SLAF) sequencing

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