Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean

Abstract

In soybean, an environmentally stable male sterility system is vital for making hybrid seed production commercially viable. Eleven male-sterile, female-fertile mutants (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS, and msp) have been identified in soybean. Of these, eight (ms2, ms3, ms5, ms7, ms8, ms9, msMOS, and msp) have been mapped to soybean chromosomes. The objectives of this study were to (i) locate the ms1, ms4, and ms6 genes to soybean chromosomes; (ii) generate genetic linkage maps of the regions containing these genes; and (iii) develop a comprehensive map of all known male-sterile, female-fertile genes in soybean. The bulked segregant analysis technique was used to locate genes to soybean chromosomes. Microsatellite markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The ms1 and ms6 genes were located on chromosome 13 (molecular linkage group F) and ms4 was present on chromosome 2 (molecular linkage group D1b). Molecular analyses revealed markers Satt516, BARCSOYSSR_02_1539, and AW186493 were located closest to ms1, ms4, and ms6, respectively. The ms1 and ms6 genes, although present on the same chromosome, were independently assorting with a genetic distance of 73.7 cM. Using information from this study and compiled information from previously published male sterility genes in soybean, a comprehensive genetic linkage map was generated. Eleven male sterility genes were present on seven soybean chromosomes. Four genes were present in two regions on chromosome 2 (molecular linkage group D1b) and two genes were present on chromosome 13 (molecular linkage group F).

Effect of marker segregation distortion on high density linkage map construction and QTL mapping in Soybean (Glycine max L.)

“…Obviously, the distortion may be caused by gametic selection. This hypothesis is partly supported by the presence of male sterile genes on chromosomes 2 and 13 (Jin et al 1998;Yang et al 2014). Based on the above results, we doubt that there are female and male sterile genes, respectively, in chromosomes 6 and 19.…”

Development of a cytoplasmic male‐sterile line NJCMS4A for hybrid soybean production

“…In soybean, 11 nuclear male‐sterile mutants ( ms1 , ms2 , ms3 , ms4 , ms5 , ms6 , ms7 , ms8 , ms9 , msMOS and msp ) have been identified and studied (Yang et al., ). These mutants have been used mostly in population development and recurrent selection but not in hybrid seed production.…”

Male sterility in soybean: Occurrence, molecular basis and utilization

“…were close to ms1, ms3 ms4 and ms6, respectively (Cervantes- Martinez et al, 2009;Yang et al, 2014). In addition to this, the fe- Weigand, Perez, Palmer, & Sandhu, 2010;Ott et al, 2013;Yang et al, 2014), and ms6 gene showed linkage with the flower colour locus w1 (Palmer, Holland, & Lewers, 1998;Skorupska & Palmer, 1989). The MFMF gene from male-sterile and female-sterile soybean mutant was mapped to LG "D1a", close to the marker, Satt531 with 9.4 cM distance from the gene (Baumbach et al, 2012).…”

“…The ms1 and ms6 genes were located on LG “F”, and ms3 and ms4 genes were located on LG “D1b”. Molecular analyses revealed that the markers Satt516, Satt157, Satt542 BARCSOYSSR_02_1539 and AW186493 were close to ms1 , ms3 ms4 and ms6 , respectively (Cervantes‐Martinez et al, ; Yang et al, ). In addition to this, the female‐partial sterile‐1 ( Fsp1 ) and the Midwest Oilseed male‐sterile ( msMOS ) mutants observed to locate on LG “D1b” and Fsp1 , whilst the location of msMOS and ms3 was noticed in close proximity of marker Satt157 (Cervantes‐Martinez et al, ).…”

“…The ms2 gene positioned on LG “O” between markers Sat_190 and Scaa001 with a distance of 6.9 and 9.0 cM, respectively (Cervantes‐Martinez et al, ). Moreover, ms5 , ms7 , ms8 and ms9 were mapped to LG “B1, K, M and N”, respectively (Cervantes‐Martinez et al, ; Frasch, Weigand, Perez, Palmer, & Sandhu, ; Ott et al, ; Yang et al, ), and ms6 gene showed linkage with the flower colour locus w1 (Palmer, Holland, & Lewers, ; Skorupska & Palmer, ). The MFMF gene from male‐sterile and female‐sterile soybean mutant was mapped to LG “D1a”, close to the marker, Satt531 with 9.4 cM distance from the gene (Baumbach et al, ).…”