Molecular markers and doubled haploids in European plant breeding programmes

Tuvesson, Stine; Dayteg, Christophe; Hagberg, P.; Manninen, Outi; Tanhuanpää, Pirjo; Tenhola-Roininen, Teija; Kiviharju, Elina; Weyen, J.; Förster, Jutta; Schondelmaier, J.; Lafferty, Julia; Marn, Marion; Fleck, Andreas

doi:10.1007/s10681-006-9239-8

Cited by 50 publications

(39 citation statements)

References 30 publications

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“…Major reductions in the time needed for cultivar development have been realized, since the availability of DHs eliminates the need for the 5-7 generations of selfing traditionally required to produce inbred lines [14]. In combination with marker assisted selection (MAS), DH induction has significantly increased the efficiency of backcross breeding [30]. By applying DH induction to one of the early backcross generations, genotypes carrying the trait to be introgressed, as well as having the highest possible proportion of the elite genome, can be selected quickly.…”

Section: Haploids and Doubled Haploids: Their Production And Use In Bmentioning

confidence: 99%

“…mapping and have been extensively used in many crops [117]. Tuvesson et al describe how a DH mapping population for marker-trait associations can be created and maintained in rye, a crop which, like perennial ryegrass, suffers from inbreeding depression [30]. Two distinct DHs are used to produce an F1 population, individuals of which are then subjected to DH induction.…”

Section: Population Development For Mappingmentioning

confidence: 99%

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Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

2016

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Abstract:The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium perenne L.), an important forage species, have advanced to a sufficiently successful and promising stage to merit an exploration of what their further developments may bring. The exploitation of both in vitro and in vivo haploid and DH methods to (1) purge deleterious alleles from germplasm intended for breeding; (2) develop mapping populations for genetic and genomic studies; (3) simplify haplotype mapping; (4) fix transgenes and mutations for functional gene validation and molecular breeding; and (5) hybrid cultivar development are discussed. Even with the comparatively modest budgets of those active in forage crop improvement, haploid and DH techniques can be developed into powerful tools to achieve the acceleration of the speed of genetic gain needed to meet future agricultural demands.

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Section: Haploids and Doubled Haploids: Their Production And Use In Bmentioning

confidence: 99%

Section: Population Development For Mappingmentioning

confidence: 99%

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

2016

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“…One application which is possible even without the genetic mapping of markers, is the control of genetic diversity within the pool of potential crossing parents in order to assure highest possible diversity within a cross, if several options are available (Tuvesson et al 2007). If the mapping position of the marker is known and many parents and their progenies are tested with markers, it is possible to follow chromosome fragments in the pedigrees, thereby identifying fragments conferring 'positive' or 'negative' characteristics (Tuvesson et al 2007). In MAS, the most straightforward application is to select for an allele of a linked marker in order to select for an allele of the linked gene (Collard et al 2005).…”

Section: What Have Markers To Offer?mentioning

confidence: 99%

The role of molecular markers and marker assisted selection in breeding for organic agriculture

et al. 2010

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Plant geneticists consider molecular marker assisted selection a useful additional tool in plant breeding programs to make selection more efficient. Standards for organic agriculture do not exclude the use of molecular markers as such, however for the organic sector the appropriateness of molecular markers is not self-evident and is often debated. Organic and low-input farming conditions require breeding for robust and flexible varieties, which may be hampered by too much focus on the molecular level. Pros and contras for application of molecular markers in breeding for organic agriculture was the topic of a recent European plant breeding workshop. The participants evaluated strengths, weaknesses, opportunities, and threats of the use of molecular markers and we formalized their inputs into breeder's perspectives and perspectives seen from the organic sector's standpoint. Clear strengths were identified, e.g. better knowledge about gene pool of breeding material, more efficient introgression of new resistance genes from wild relatives and testing pyramided genes. There were also common concerns among breeders aiming at breeding for organic and/ or conventional agriculture, such as the increasing competition and cost investments to get access to marker technology, and the need for bridging the gap between phenotyping and genotyping especially with complex and quantitative inherited traits such as nutrient-efficiency. A major conclusion of the authors is that more interaction and mutual understanding between organic and molecular oriented breeders is necessary and can benefit both research communities.

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“…A large number of markers associated with QTLs/genes for resistance/tolerance to biotic/abiotic stress as well as agronomic traits are reported every year in the form of research publications. However transfer of markers to practical plant breeding, in the same proportion has not taken place at all or took longer than expected (Tuvesson et al 2007). As a result, lots of claims have been made about what great things genomics does, but very little has been put into application (Varshney and Tuberosa 2007).…”

Section: Superiority Of Fms Over Traditional Markers In Masmentioning

confidence: 99%

Gene-Based Marker Systems in Plants: High Throughput Approaches for Marker Discovery and Genotyping

Varshney

2009

Molecular Techniques in Crop Improvement

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Development and application of molecular markers derived from genes, commonly called genic markers or sometimes functional markers, is gaining momentum in plant genetics and breeding. Availability of large amount of sequence data coming from genome/transcriptome sequencing projects as well as advent of next generation sequencing technologies together with advances in bioinformatics tools, marker discovery is becoming cheaper and faster. The availability of inexpensive high-density SNP-genotyping arrays is encouraging the plant genetics and breeding community to undertake genome-wide marker genotyping for a variety of applications. For instance, high-throughput and low cost genotyping assays for gene-based markers offers the possibility to accelerate the trait mapping based on high-density linkage mapping as well as genome-scanning based association mapping approaches in addition to facilitate physical mapping, comparative mapping, phylogenetic studies and understanding genome organization in crop plant species. Marker discovery, genotyping and molecular breeding practices would be routine in near future for crop improvement in many crop species. Advances in the area of marker discovery and genotyping using highly parallel genomics assays and also a few applications have been discussed in this chapter.

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Molecular markers and doubled haploids in European plant breeding programmes

Cited by 50 publications

References 30 publications

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

The role of molecular markers and marker assisted selection in breeding for organic agriculture

Gene-Based Marker Systems in Plants: High Throughput Approaches for Marker Discovery and Genotyping

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