Molecular markers for animal biotechnology: sea bass (Dicentrarchus labrax, L.) HMG-CoA reductase mRNA

Gornati, Rosalba; Papis, Elena; Rimoldi, Simona; Chini, Valentina; Terova, Genciana; Saroglia, Marco; Bernardini, Giovanni

doi:10.1016/j.gene.2004.11.007

Cited by 33 publications

(19 citation statements)

References 24 publications

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“…Similar patterns were also found in other fish species such as sturgeon, (Acipenser baerii) [42] and sea bass (Dicentrarchus labrax) after short-term exposure to stress [43].…”

Section: Discussionsupporting

confidence: 82%

Untitled

2017

JESR

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Exposure to any type of stressors either environmental, chemical or perceived stressors, results in a series of physiological responses in animals. The purpose of this study was to investigate the effects of thermal stress on physiological responses in red hybrid tilapia. Adult red hybrid tilapia were previously exposed to gradual increment of water temperature in thermoregulated recirculating tanks at a rate of 1 o C/8 h from 28 to 31 o C and kept for 1, 7 and 14 days. Cortisol, HMG-CoA reductase, total protein and osmolality were determined. Plasma cortisol and HMG-CoA levels were significantly increased in the heat-stressed groups compared to nonstressed groups. Osmolality was also significantly higher (p<0.05) at day 14 in heat-stressed groups compared to non-stressed groups. Our study demonstrated that exposure to elevated water temperature affect physiological parameters in red hybrid tilapia, thus suggesting a potential threat to its biological performance.

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“…Similar patterns were also found in other fish species such as sturgeon, (Acipenser baerii) [42] and sea bass (Dicentrarchus labrax) after short-term exposure to stress [43].…”

Section: Discussionsupporting

confidence: 82%

Untitled

2017

JESR

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“…HMGR expression could be increased by pathological conditions such as tumors (Hentosh, Yuh, Elson, & Peffley, 2001) and pentachlorophenol (Svensson et al, 2003;Deng et al, 2014). Moreover, enhanced expression of heat shock protein (HSP) and HMGR has been shown after heat stress (Zager & Johnson, 2001) and crowding stress (Gornati et al, 2005). In the present study, up-regulated expression of HMGR by oxidized fish oil was associated with the histological damage of liver.…”

Section: Discussionsupporting

confidence: 49%

“…In the present study, profound alterations in liver histology of grass carp were noticed after feeding oxidized fish oil. Due to the conserved role of HMGR, its expression can be a good marker for detecting fish health (Gornati et al, 2005). HMGR expression could be increased by pathological conditions such as tumors (Hentosh, Yuh, Elson, & Peffley, 2001) and pentachlorophenol (Svensson et al, 2003;Deng et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…3-Hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR) is a rate-limiting enzyme for cholesterol biosynthesis, catalyzing conversion of HMG-CoA to mevalonic acid (Norambuena et al, 2013). It has been reported that HMGR is a good marker of stress response and tissue damage in fish because of its conversed N-and C-terminus portions (Chen et al, 2012a;Gornati et al, 2005). However, to date there is very little available information on fish HMGR.…”

Section: Introductionmentioning

confidence: 99%

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Ye¹,

Huang²,

Ye³

2018

Turk. J. Fish. Aquat. Sci.

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Abstract3-Hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR) is a rate-limiting enzyme for cholesterol biosynthesis. The aim of this study was to identify HMGR molecular characterization in grass carp (Ctenopharyngodon idellus), and to evaluate regulation of its expression by oxidized fish oil. Specific primers were designed for 3'RACE and 5'RACE to gain HMGR cDNA sequence of grass carp and to clone full-length HMGR cDNA. This cDNA covered 3591 bp, with 2526 bp for the open reading frame (ORF), 77 bp for 5'-untranslated region (UTR) and 988 bp for 3'UTR. ORF encoded a protein consisting of 841 amino acids. The result revealed that HMGR mRNA is predominantly expressed in liver. Multiple alignments and phylogenetic analysis indicated that grass carp HMGR has a high degree of conservation with other fish species. A 10-week feeding trial was conducted to investigate effects of offering diets containing fresh and oxidized fish oil on HMGR expression in liver and intestine of grass carp. The results showed that HMGR expression in fish fed the diet containing oxidized fish oil was significantly higher than fish fed the fresh fish oil after 10, 20, 30 and 70 days of feeding. Serum diamineoxidase (DAO) activity of fish was also increased by oxidized fish oil.

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“…Podrabsky and Somero (2007) found that an inducible or embryo-specific form of HSP70 is expressed during embryonic development in killifish (Austrofundulus limnaeus) and is elevated during diapause II in this species. Gornati et al (2004Gornati et al ( , 2005 detected a different expression of HSP70 and HSP90 mRNAs in sea bass farmed at different stocking densities. Moreover, handling and stocking density procedures can affect cortisol levels as suggested by several authors (Marino et al 2001, Skjervold et al 2001, Simontacchi et al 2008.…”

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confidence: 99%

Immunohistochemical localization of constitutive and inducible Heat Shock Protein 70 in carp (Cyprinus carpio) and trout (Oncorhynchus mykiss) exposed to transport stress

Poltronieri¹,

Negrato²,

Bertotto³

et al. 2009

Eur J Histochem

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In the present work we investigated by immunohistochemistry the cellular localization of constitutive as well as inducible heat shock protein 70 in several tissues of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) exposed to transport stress. In carp, the constitutive form (HSC70) was detected only in red skeletal muscle of both control and stressed animals. In the same species, the inducible form (HSP70) was evident in the epithelia of renal tubules, gills and skin of stressed animals, whereas in controls only red skeletal muscle exhibited an immunopositivity to HSP70 antibody. In trout, immunostaining to HSC70 antibody was found mainly in the epithelia of intestine, gills and skin of both control and stressed animals although the reactivity was generally higher in animals exposed to transport stress. In the same species immunostaining to HSP70 antibody was observed only in red skeletal muscle and epidermis of control animals.

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Molecular markers for animal biotechnology: sea bass (Dicentrarchus labrax, L.) HMG-CoA reductase mRNA

Cited by 33 publications

References 24 publications

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Immunohistochemical localization of constitutive and inducible Heat Shock Protein 70 in carp (Cyprinus carpio) and trout (Oncorhynchus mykiss) exposed to transport stress

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