Molecular markers for oocyte competence in bovine cumulus cells

Kussano, N. R.; Leme, L. O.; Guimarães, A. L. S.; Franco, M. M.; Dode, M. A. N.

doi:10.1016/j.theriogenology.2015.11.033

Cited by 27 publications

(29 citation statements)

References 72 publications

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“…In the present study, the effect of FF on the cumulus cells was evaluated by determining the expression of marker genes. GPC4 encodes a heparin sulfate proteoglycan that is associated with the cell surface and is reported in cow as a cumulus cell marker for oocyte developmental competence . Furthermore, the expression levels of the PFKP and PRDX3 genes in cumulus cells are associated with good‐quality embryos in humans; this study's results showed that the expression levels of these genes were higher in the cumulus cells that had been cultured in FF medium.…”

Section: Discussionmentioning

confidence: 63%

“…The expression levels of several genes in cumulus cells are associated with oocyte developmental competence . In the present study, the effect of FF on the cumulus cells was evaluated by determining the expression of marker genes.…”

Section: Discussionmentioning

confidence: 98%

“…The expression levels of several genes in cumulus cells are associated with oocyte developmental competence. [28][29][30][31] In the present study, the effect of FF on the cumulus cells was evaluated by determining the expression of marker genes. GPC4 encodes a heparin sulfate proteoglycan that is associated with the cell surface and is reported in cow as a cumulus cell marker for oocyte developmental competence.…”

Section: Discussionmentioning

confidence: 99%

“…GPC4 encodes a heparin sulfate proteoglycan that is associated with the cell surface and is reported in cow as a cumulus cell marker for oocyte developmental competence. 31 Furthermore, the expression levels of the PFKP and PRDX3 genes in cumulus cells are associated with good-quality embryos in humans 29 ;…”

Section: Discussionmentioning

confidence: 99%

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Non‐esterified fatty acid‐associated ability of follicular fluid to support porcine oocyte maturation and development

Ogawa

Itami

Ueda

et al. 2018

Reprod Medicine & Biology

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PurposeThe effect of supplementing maturation medium with follicular fluid (FF) was examined according to its non‐esterified fatty acid (NEFA) content or with a fatty acid mixture (FA‐Mix) on the developmental competence of oocytes, as well as the mitochondrial quality and quantity in the oocytes and cumulus cells.MethodPorcine oocytes from a slaughterhouse were used.ResultsThe FF or FA‐Mix in maturation medium increased the lipid content in both the oocytes and the cumulus cells, but the adenosine triphosphate content was differentially affected. The FF supplementation increased the mitochondrial DNA copy number, survival of cumulus cells, and rate of oocyte development to the blastocyst stage, whereas the FA‐Mix supplementation did not show these effects. The expression levels of GPC4,PFKP,PRDX3, and TFAM in the cumulus cells increased after FF supplementation, but the expression of GJA1 decreased, compared with the cells that were cultured without FF.ConclusionAdding FF and FA‐Mix to the maturation medium increased the lipid content in the oocytes and cumulus cells. The effects of FF on the cumulus cells and oocytes were not observed after FA‐Mix supplementation, indicating that the concentration of the NEFAs in the FF are closely associated with an ability to support oocyte maturation and the metabolism of cumulus cells and oocytes.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Non‐esterified fatty acid‐associated ability of follicular fluid to support porcine oocyte maturation and development

Ogawa

Itami

Ueda

et al. 2018

Reprod Medicine & Biology

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“…Posteriormente, Los COC fueron colocados en una caja de Petri con ayuda de una pipeta Pasteur para su posterior localización y selección. Los ovarios y los COC fueron mantenidos durante el proceso de colección a 30 grados centígrados utilizando un baño de Maria 19 .…”

Section: Materiales Y Métodosunclassified

Producción in vitro de embriones a partir de complejos cúmulos oocitos tipo II en bovinos Bos indicus

Velez¹,

Chica²,

Urrego³

et al. 2017

Ces. Med. Vet. Zootec.

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The morphological selection of cumulus-oocyte complexes (COCs) is an important step for in vitro embryo production. It has been suggested that COC showing signs of atresia have the ability to generate embryos. The objective of this work was to evaluate the effect of COC morphology from Bos indicus animals with signs of early atresia versus no signs of atresia on in vitro embryo production. COC were classified in: Type I (TI): homogeneous ooplasm with ≥ 4 layers of compact cumulus cells (CC) and Type II (TII): granular ooplasm and ≥ 4 layers of CC slightly expanded. The COC were matured in vitro for 24 hours in TCM199 medium and subsequently fertilized in vitro for 18 h. The suspected zygotes were cultured in vitro for seven days in modified SOFaa medium. Embryonic quality was determined by blastomeric count following staining with Hoechst 33342. Student test was used to determine statistical differences for cleavage, blastocyst rate and blastomeric counts between types of COC. The cleavage rate for TI (n = 220) and TII (n = 161) was 88 ± 4 % and 89 ± 8 % respectively (p> 0.05); embryo development rate was 36 ± 7 % and 33 ± 8 % (p>0.05) respectively. The blastomeric count for both groups was 101 and 104 cells for TI and TII respectively (n = 10), (p>0.05). These results demonstrate that there is no difference in the quantity and quality of embryos produced in vitro using COC type I or type II, suggesting that both types could be used for bovine in vitro embryo production in Bos indicus cows. Keywords: atresia, competence, cumulus-oocyte complex.Resumen La selección morfológica de los complejos cúmulo-oocito (COC) es crucial para la producción de embriones in vitro. Se ha sugerido que COC que muestran signos de atresia poseen capacidad de generar embriones. El objetivo de este trabajo fue evaluar el efecto de la morfología de los COC provenientes de animales Bos indicus con signos de atresia temprana y sin signos de atresia sobre la producción de embriones in vitro. Se clasificaron COC obtenidos de ovarios de faenado en dos grupos: Tipo I (TI): ooplasma homogéneo con ≥ 4 capas de células del cumulo (CC) compactas y Tipo II (TII): ooplasma

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RNA‐sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells

Martínez-Moro

González-Brusi²,

Lamas-Toranzo³

et al. 2022

Molecular Reproduction Devel

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Cumulus cells provide an interesting biological material to perform analyses to understand the molecular clues determining oocyte competence. The objective of this study was to analyze the transcriptional differences between cumulus cells from oocytes exhibiting different developmental potentials following individual in vitro embryo production by RNA‐seq. Cumulus cells were allocated into three groups according to the developmental potential of the oocyte following fertilization: (1) oocytes developing to blastocysts (Bl+), (2) oocytes cleaving but arresting development before the blastocyst stage (Bl−), and (3) oocytes not cleaving (Cl−). RNAseq was performed on 4 (Cl−) or 5 samples (Bl+ and Bl−) of cumulus cells pooled from 10 cumulus‐oocyte complexes per group. A total of 49, 50, and 18 differentially expressed genes (DEGs) were detected in the comparisons Bl+ versus Bl−, Bl+ versus Cl− and Bl‐ versus Cl−, respectively, showing a fold change greater than 1.5 at an adjusted p value <0.05. Focussing on DEGs in cumulus cells from Bl+ group, 10 DEGs were common to both comparisons (10/49 from Bl+ vs. Bl−, 10/50 from Bl+ vs. Cl−). These DEGs correspond to 6 upregulated genes (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, and SLC1A4), and 4 downregulated genes (GSTA1, PSMB8, FMOD, and SFRP4) in Bl+ compared to the other groups, from which 7 were validated by quantitative PCR (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, PSMB8 and SFRP4). These genes are involved in critical biological functions such as integrin‐mediated cell adhesion, oxygen availability, IGF and Wnt signaling or PKA pathway, highlighting specific biological processes altered in incompetent in vitro maturation oocytes.

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Molecular markers for oocyte competence in bovine cumulus cells

Cited by 27 publications

References 72 publications

Non‐esterified fatty acid‐associated ability of follicular fluid to support porcine oocyte maturation and development

Non‐esterified fatty acid‐associated ability of follicular fluid to support porcine oocyte maturation and development

Producción in vitro de embriones a partir de complejos cúmulos oocitos tipo II en bovinos Bos indicus

RNA‐sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells

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