Molecular markers for ozone stress isolated by suppression subtractive hybridization: specificity of gene expression and identification of a novel stress‐regulated gene

Sävenstrand, Helena; Brosché, Mikael; Ängehagen, M.; Strid, Åke

doi:10.1046/j.1365-3040.2000.00586.x

Cited by 39 publications

(26 citation statements)

References 35 publications

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“…Transcript abundance of these genes, as expected, were mostly upregulated in response to Al, however, three previously reported genes showed differing expression patterns in Arabidopsis. Transcripts for leucine-rich repeated protein (Savenstrand et al 2000) and PAL (Snowden and Gardner et al 1993) were reported to be up-regulated in response to Al, both genes showed decreased expression in our study. The gene for alanine aminotransferase was up-regulated in our study while, Richards et al (1998) reported the down-regulation of this gene in Arabidopsis in response to Al.…”

Section: Validation Of Microarray Resultssupporting

confidence: 51%

Microarray analysis of Arabidopsis genome response to aluminum stress

Goodwin¹,

Sutter²

2009

Biologia plant.

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To better understand the mechanisms involved in aluminum toxicity and tolerance in plants, microarray technology was used to evaluate changes in gene expression in Arabidopsis thaliana under Al stress. With the use of Affymetrix Arabidopsis ATH1 Genechip, a comparison of RNA expression profiles was made between control and Al-treated Arabidopsis seedlings. A total of 256 genes were identified as Al-responsive. Ninety-four genes were shown to be up-regulated and 162 were down-regulated; comprising 1.1 % of the 24 000 Arabidopsis genes. Real-time RT-PCR was used to confirm the microarray data. The analysis showed that a large number of transcription factors and several putative signaling components were up-regulated by aluminum. Chloroplast structural and photosynthetic genes were, in general, down-regulated. A number of previously identified Al-responsive genes, e.g. GST, Auxin-regulated, Peroxidase, and Chitinase, were up-regulated by Al-stress, whereas Wali 3 and Wali 4 were down-regulated. We also identified several up-regulated genes involved in vacuolar signaling, sorting and docking. Three genes were also up-regulated by Al-stress, Ras GTP-binding protein, ABC-cassette binding, and the AtELP1 receptor genes, have previously been documented as responsive to drought and/or oxidative stress and may play important roles the detoxification of Al ions by transportation and storage into root vacuoles. Ultrastructural changes in the roots tips cells of Arabidopsis were evaluated using transmission electron microscopy and energy-dispersive X-ray analysis with scanning electron microscopy and results showed Al accumulation in the root tips of Arabidopsis.

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Section: Validation Of Microarray Resultssupporting

confidence: 51%

Microarray analysis of Arabidopsis genome response to aluminum stress

Goodwin¹,

Sutter²

2009

Biologia plant.

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“…ozone-specific gene regulation pattern, or the development of a molecular probe for ozone stress [16]. Though a variety of plant species have been examined in their response(s) to ozone [15], the rice (Oryza sativa L.) plant, a monocot research model (cv.…”

Section: Introductionmentioning

confidence: 99%

Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings

et al. 2002

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Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate peroxidase protein(s) was accompanied by rapid changes in the 2-DE protein profiles, over controls. Out of a total of 56 proteins investigated, which were reproducible in repeated experiments, 52 protein spots were visually identified as differentially expressed over controls. Six proteins were N-terminally blocked, and the sequence of 14 proteins could not be determined, whereas 36 proteins were N-terminally and one was internally sequenced. Ozone caused drastic reductions in the major leaf photosynthetic proteins, including the abundantly present ribulose-1, 5-bisphosphate carboxylase/oxygenase, and induction of various defense/stress related proteins. Most prominent change in leaves, within 24 h post-treatment with ozone, was the induced accumulation of a pathogenesis related (PR) class 5 protein, three PR 10 class proteins, ascorbate peroxidase(s), superoxide dismutase, calcium-binding protein, calreticulin, a novel ATP-dependent CLP protease, and an unknown protein. Present results demonstrate the highly damaging effect of ozone on rice seedlings at the level of the proteome.

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“…Since the cloning of the Wali genes (Snowden and Gardner, 1993;, other genes have been shown to respond to Al stress, such as those identified in rice (Oryza sativa; Yu et al, 1998), tobacco (Nicotiana tabacum; Ezaki et al, 1995;Ezaki et al, 1996), wheat (Triticum aestivum; Cruz-Ortega et al, 1997;Hamel et al, 1998;Delhaize et al, 1999;Hamilton et al, 2001), Arabidopsis (Richards et al, 1998), and pea (Pisum sativum; Brosché and Strid, 1999;Sävenstrand et al, 2000). These studies provided an initial description of potentially important genes involved in Al stress.…”

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confidence: 99%

Expressed Sequence Tag-Based Gene Expression Analysis under Aluminum Stress in Rye,

Rodríguez-Milla¹,

Butler

Huete³

et al. 2002

Plant Physiology

100

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To understand the mechanisms responsible for aluminum (Al) toxicity and tolerance in plants, an expressed sequence tag (EST) approach was used to analyze changes in gene expression in roots of rye (Secale cereale L. cv Blanco) under Al stress. Two cDNA libraries were constructed (Al stressed and unstressed), and a total of 1,194 and 774 ESTs were generated, respectively. The putative proteins encoded by these cDNAs were uncovered by Basic Local Alignment Search Tool searches, and those ESTs showing similarity to proteins of known function were classified according to 13 different functional categories. A total of 671 known function genes were used to analyze the gene expression patterns in rye cv Blanco root tips under Al stress. Many of the previously identified Al-responsive genes showed expression differences between the libraries within 6 h of Al stress. Certain genes were selected, and their expression profiles were studied during a 48-h period using northern analysis. A total of 13 novel genes involved in cell elongation and division (tonoplast aquaporin and ubiquitin-like protein SMT3), oxidative stress (glutathione peroxidase, glucose-6-phosphate-dehydrogenase, and ascorbate peroxidase), iron metabolism (iron deficiency-specific proteins IDS3a, IDS3b, and IDS1; S-adenosyl methionine synthase; and methionine synthase), and other cellular mechanisms (pathogenesis-related protein 1.2, heme oxygenase, and epoxide hydrolase) were demonstrated to be regulated by Al stress. These genes provide new insights about the response of Al-tolerant plants to toxic levels of Al.Al is one of the most important limiting factors for crop production on acid soils. The most important effect of Al toxicity is a dramatic reduction in root growth, which leads to poor productivity. Severe Al stress threatens the survival of many sensitive crop genotypes. Al has been shown to affect a large number of cellular processes, especially the uptake of K (Jones and Kochian, 1995). Al also induces the secretion of organic acids (e.g. citrate, malate, and oxalate) from roots (Delhaize and Ryan, 1995). These organic acids form a stable complex with Al, preventing the toxic effects of Al and providing the most valuable source of tolerance in the majority of plant species studied. Despite the considerable progress made over the last decade, the rather modest progress in isolating Al-regulated genes has limited our understanding of the molecular mechanisms underlying Al toxicity and tolerance.Changes in gene expression control normal physiological processes and are also the main effectors of cellular responses to biotic or abiotic stresses (Jiang et al., 2000). Since the cloning of the Wali genes (Snowden and Gardner, 1993;, other genes have been shown to respond to Al stress, such as those identified in rice (Oryza sativa; Yu et al., 1998), tobacco (Nicotiana tabacum; Ezaki et al., 1995;Ezaki et al., 1996), wheat (Triticum aestivum; Cruz-Ortega et al., 1997;Hamel et al., 1998;Delhaize et al., 1999;Hamilton et al., 2001), Arabidopsis (Richards...

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Molecular markers for ozone stress isolated by suppression subtractive hybridization: specificity of gene expression and identification of a novel stress‐regulated gene

Cited by 39 publications

References 35 publications

Microarray analysis of Arabidopsis genome response to aluminum stress

Microarray analysis of Arabidopsis genome response to aluminum stress

Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings

Expressed Sequence Tag-Based Gene Expression Analysis under Aluminum Stress in Rye,

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