Molecular Markers in Assessing Genetic Clonal Fidelity for in Vitro Propagated Endangered Medicinal Plants

Biswas, Protha; Nandy, Samapika; Dey, Abhijit; Tikendra, Leimapokpam; Nongdam, Potshangbam

doi:10.1007/978-981-16-6005-4_6

Cited by 7 publications

(4 citation statements)

References 175 publications

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“…3 and 4). These results were expected due to the fact that microshoots are differentiated tissues that are known (like any other differentiated tissue) for their ability to remain true type even after being undergone tissue culturing systems (Tikendra et al, 2019;Zhang et al, 2023;Biswas et al, 2023). Luo et al (2023) confirmed the genetic stability in their study on Manglietiastrum sinicum when RAPD or ISSR markers were used after in vitro propagation.…”

Section: Effect Of In Vitro Propagation On Genetic Stability Of S Nig...mentioning

confidence: 71%

Evaluation of genetic diversity in Jordanian Solanum nigrum plants and genetic stability of invitro grown plant using (AFLP) technique

2024

JJBS

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Solanum nigrum is an herbal plant that is reported for its healing powers against many ailments. In Jordan S. nigrum grows wild as a toxic weed in neglected fields and without any consideration of its medicinal value. This research aimed to collect and examine the genetic relationships of S. nigrum from different sites in Jordan. The in vitro-grown S. nigrum was established from the collected wild populations. Results of wild populations of S. nigrum. were arranged in two clusters (A & B) at the top range of hierarchy (genetic similarity of 0.13). Cluster A separated Bergesh Reserve population into its own group, while cluster B contained the remaining populations which were divided into two sub-clusters at a genetic similarity of 0.65, B1 contained Deer Alla and Jerash (2014 + 2015) populations at genetic similarity of 0.69 and B2 contained Almojeb Valley and Salt populations at genetic similarity of 0.71. However, the highest genetic similarity (0.87) was obtained between Jerash 2014 and Jerash 2015 from the same location. This dissimilarity might be due to the probability of cross-pollination AFLP (Amplified Fragment Length Polymorphism) molecular analysis gave no genetic variations between in vitro grown S. nigrum before and after micropropagation compared with wild-type mother plants collected from the Jerash site in 2014. A solid conclusion needs more research on the genetic diversity of Jordanian wild-grown S. nigrum. This can be achieved by extending the collection scale to include all sites where this valuable plant grows.

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Section: Effect Of In Vitro Propagation On Genetic Stability Of S Nig...mentioning

confidence: 71%

Evaluation of genetic diversity in Jordanian Solanum nigrum plants and genetic stability of invitro grown plant using (AFLP) technique

2024

JJBS

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Section: Effect Of In Vitro Propagation On Genetic Stability Of S Nig...mentioning

confidence: 91%

“…3 and 4). However, genetic alterations in tissue-cultured plant materials reported in some studies were rare and referred to personal error rather than the tissue culture technique itself and described to be epigenetic as the original DNA was not altered (Smulders and Klerk, 2011;Biswas et al, 2023).The tissue culture of plants could be affected by somaclonal variation and other factors like the type of plant hormones used, the time of in vitro propagation, direct-versus indirect regeneration, etc (Biswas et al, 2023;Bulbarela et al, 2023). However, most reports found a low percentage of soma clonal variation occurrences in tissue-cultured plants (Taji et al, 2002;Duta-Cornescu et al, 2023).…”

Section: Effect Of In Vitro Propagation On Genetic Stability Of S Nig...mentioning

confidence: 99%

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2023

JJBS

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Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscript that meets the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately one to two months after acceptance.

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“…In the plant tissue culture studies, the genetic homogeneity of the in vitro propagated plants in most cases is still constant. The soma-clonal variation in the plant tissue culture is affected by the growth regulators used and the direct and indirect regeneration [61,62]. However, most reports found a low percentage of somaclonal variation occurrence in tissue-cultured plants [63,64].…”

Section: Cryopreservation Of S Dominica Callusmentioning

confidence: 99%

A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

Al-Qudah,

Shibli,

Zatimeh

et al. 2023

Sustainability

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Salvia dominica L. is an important wild medicinal plant that grows in Jordan and neighboring countries, and this plant has been suffering from many threats in its wild environment. Therefore, this research aims to establish a comprehensive and sustainable approach via an in vitro propagation and conservation system for the S. dominica L. plant. Axillary buds were used to initiate the in vitro culture on Murashige and Skoog MS media supplemented with 0.5 mg L−1 of GA3. In vitro shoot proliferation and rooting were experimented on with different concentrations of cytokinins and auxins, respectively. Calli were induced in the dark on excised leaf discs (0.5 cm in diameter), and multiplication was experimented on with different growth regulators. Cryopreservation experiments were applied on the callused segments under different growth conditions via the vitrification technique. A full protocol was achieved for shoot proliferation with 6.3 shoots/explant using 1.2 mg L−1 of thidiazuron (TDZ), while rooting was achieved at 1.5 mg L−1 of NAA with 6.6 functional roots/explant. Acclimatization was completely successful for the rooted plants. The highest callus production with 5.81 g/calli was achieved using 1.5 mg L−1 of benzylaminopurine (BAP). Cryopreservation of the S. dominica calli was successfully achieved when a pure plant vitrification solution (PVS2) was used to dehydrate the calli for 20 min after immersion in the loading solution for 20 min with a 76.6% regrowth percentage. The loading and the plant vitrification solution type and duration were the most critical points in the regrowth of the cryopreserved calli. In conclusion, a successful protocol was set up for the in vitro propagation and conservation of S. dominica calli. This study has prompted us to perform further studies on sustainable in vitro production and conservation of critically endangered medicinal plants to implement a green environment protecting against surrounding threats.

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Molecular Markers in Assessing Genetic Clonal Fidelity for in Vitro Propagated Endangered Medicinal Plants

Cited by 7 publications

References 175 publications

Evaluation of genetic diversity in Jordanian Solanum nigrum plants and genetic stability of invitro grown plant using (AFLP) technique

Evaluation of genetic diversity in Jordanian Solanum nigrum plants and genetic stability of invitro grown plant using (AFLP) technique

Untitled

A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

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