Molecular markers to differentiate two morphologically‐close species of the genus <i>Sitobion</i>

Figueroa, Christian C.; Simon, Jean‐Christophe; Gallic, Jean-François Le; Niemeyer, Hermann M.

doi:10.1046/j.1570-7458.1999.00540.x

Cited by 18 publications

(14 citation statements)

References 27 publications

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“…In total, 11 different gene regions were targeted for this study for potential sequence variation across species: 1) ATP synthase subunit a (ATPsa); 2) ATP synthase subunit b; 3) adenine nucleotide transporter translocase; 4) signal recognition particle 54; 5) tata box binding protein; 6) lysidyl aminoacyl transfer RNA synthase; 7) zinc metalloproteins; 8) cytochrome b (cyt b); 9) rDNA internal transcribed space region 2 (ITS2); 10) cytochrome oxidase subunit 1 (CO1), and 11) dopa decarboxylase gene (DDC). Sources to the universal primers used for ampliÞcation of the gene regions numbered are listed as follows: gene regions 1Ð7, Jarmon et al (2002); 8, Simon et al (1994), Figueroa et al (1999), Bulman et al (2005), and Rabaoudi et al (2002); 9, Bulman et al (2005); 10, Folmer et al (1994); and 11, Fang et al (1997).…”

Section: Methodsmentioning

confidence: 99%

Developing and Testing a Diagnostic Probe for Grape Phylloxera Applicable to Soil Samples

Herbert

Powell

McKay

et al. 2008

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Grape phylloxera, Daktulosphaira vitifoliae (Fitch) (Hemiptera Phylloxeridae) is a damaging pest of grapevines (Vitis spp.) around the world, and the management of this pest requires early detection of infestations. Here, we describe the development and validation of a sensitive DNA test for grape phylloxera that can be applied to soil. Species-specific primers were developed for grape phylloxera in the internal transcribed space region 2, and their specificity was confirmed after thorough screening by using a wide range of vineyard organisms and aphid genera. Preliminary testing of the detection limits of the grape phylloxera-specific primers was conducted using field-sourced soil types spiked with a known number of grape phylloxera. The assay was converted to a real-time polymerase chain reaction format (TaqMan MGB). This assay, in combination with DNA extraction from soil, can detect phylloxera crawlers added to soil. The assay was evaluated in the field at a recently detected grape phylloxera infestation site from the Yarra Valley in Victoria, Australia. The DNA assay proved to be substantially more sensitive than a standard ground survey for detecting grape phylloxera presence on vine roots in the infested vineyard. Moreover, unlike the ground survey, the assay provided quantitative information on grape phylloxera infestations, because grape phylloxera DNA concentrations in samples from vines closely matched the numbers of grape phylloxera crawlers collected with emergence traps placed at the base of vines. Unlike other detection techniques, the method can be applied at any time of the year, and it can be potentially modified to provide specific information on the virulence levels of the particular grape phylloxera genotypes responsible for any new infestations.

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Section: Methodsmentioning

confidence: 99%

Developing and Testing a Diagnostic Probe for Grape Phylloxera Applicable to Soil Samples

Herbert

Powell

McKay

et al. 2008

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“…As S. avenae co-occurs in Chile with its closely related species S. fragariae, Sitobion individuals were determined as S. avenae or S. fragariae according to morphological and molecular criteria (Figueroa et al, 1999). As there is evidence for hybridization/introgression between these two Sitobion species, diagnostic alleles from loci Sm10, Sm11 and Sm17 were also used to discriminate between parental species and their putative hybrids .…”

Section: Aphid Collectionmentioning

confidence: 99%

“…Previous work on the genetic structure of a restricted sample of S. avenae in Chile using mainly RAPD-PCR markers showed a low genetic diversity and a lack of host-based genetic structure (Figueroa et al, 1999(Figueroa et al, , 2002. In contrast, in European countries (eg France, UK and Romania), a high genetic variability and genetic differentiation according to the mode of reproduction and the host plant were found using microsatellite loci (De Barro et al, 1995;Sunnucks et al, 1997;Simon et al, 1999a;Llewellyn et al, 2003;Papura et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Genetic structure and clonal diversity of an introduced pest in Chile, the cereal aphid Sitobion avenae

et al. 2005

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In Chile, the aphid Sitobion avenae is of recent introduction, lives on cultivated and wild Poaceae, and is thought to reproduce by permanent parthenogenesis. In order to study the genetic variability and population structure of this species, five microsatellite loci were typed from individual aphids collected from different cultivated and wild host plants, from different geographical zones, and years. Chilean populations showed a high degree of heterozygosity and a low genetic variability across regions and years, with four predominant genotypes representing nearly 90% of the sample. This pattern of low clonal diversity and high heterozygosity was interpreted as the result of recent founder events from a few asexually reproducing genotypes. Most geographical and temporal variation observed in the genetic composition resulted from fluctuations of a few predominant clones. In addition, comparisons of the genotypes found in Chile with those described in earlier surveys of S. avenae populations in Western Europe led us to identify 'superclones' with large geographical distribution and high ecological success, and to make a preliminary exploration of the putative origin(s) of S. avenae individuals introduced to Chile. Heredity (2005) 95, 24-33.

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“…Molecular markers can be used to verify genetic variability in the field and to distinguish sibling species (Black et al 1992;Stevens and Wall 1995;Reyes and Ochando 1998;Figueroa et al 1999;Orui and Mizukubo 1999;Navajas and Fenton 2000). Other uses of molecular markers include identifying the occurrence of parasitism, and (or) endosymbionts (Weeks et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA and RAPD polymorphisms in the haploid mite Brevipalpus phoenicis (Acari: Tenuipalpidae)

et al. 2004

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Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae) is recognized as the vector of citrus leprosis virus that is a significant problem in several South American countries. Citrus leprosis has been reported from Florida in the past but no longer occurs on citrus in North America. The disease was recently reported in Central America, suggesting that B. phoenicis constitutes a potential threat to the citrus industries of North America and the Caribbean. Besides B. phoenicis, B. obovatus Donnadieu, and B. californicus (Banks) have been incriminated as vectors of citrus leprosis virus and each species has hundreds of host plants. In this study, Brevipalpus mite specimens were collected from different plants, especially citrus, in the States of Florida (USA) and São Paulo (Brazil), and reared on citrus fruit under standard laboratory conditions. Mites were taken from these colonies for DNA extraction and for morphological species identification. One hundred and two Random Amplified Polymorphic DNA (RAPD) markers were scored along with amplification and sequencing of a mitochondrial cytochrome oxidase subunit I gene fragment (374 bp). Variability among the colonies was detected with consistent congruence between both molecular data sets. The mites from the Florida and Brazilian colonies were morphologically identified as belonging to B. phoenicis, and comprise a monophyletic group. These colonies could be further diagnosed and subdivided geographically by mitochondrial DNA analysis.

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Molecular markers to differentiate two morphologically‐close species of the genus Sitobion

Cited by 18 publications

References 27 publications

Developing and Testing a Diagnostic Probe for Grape Phylloxera Applicable to Soil Samples

Developing and Testing a Diagnostic Probe for Grape Phylloxera Applicable to Soil Samples

Genetic structure and clonal diversity of an introduced pest in Chile, the cereal aphid Sitobion avenae

Mitochondrial DNA and RAPD polymorphisms in the haploid mite Brevipalpus phoenicis (Acari: Tenuipalpidae)

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