2019
DOI: 10.5958/0973-9130.2019.00471.7
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Molecular Marking of Liver DNA Methylation in Rat's with Thyroid Carcinoma

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“…In the present research, the resistance to common antibiotics used in the treatment of S. typhi was tested and appeared that all the persisting isolates of S. typhi obtained from patients who suffered typhoid fever showed high resistance to gentamycin, ampicillin amikacin, and ceftriaxone; these results agree with Andualem et al and Alaarajy et al (11,12) they were found that all isolates were resistant to four or more classes of antibiotics as antimicrobials. The present study implemented to identifyWzx and Wzy genes in Salmonella typhi isolates that have an important role in typhoid diagnosis via O-antigen, although of the low sensitivity and specificity of Widal test in typhoid diagnosis, it is still used in some labs (11), on the other hands the false negative results (13,14) may be associated with genetic diversity of O-antigen genes which formed diversity in O-antigen proteins. The evidence found that according to The Kauffmann-White scheme there are 46 somatic O antigens of Salmonella, thus the diversity of O-antigens encoded genes can be used in the PCR development test to molecular serotypes identification.…”
Section: Discussionmentioning
confidence: 99%
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“…In the present research, the resistance to common antibiotics used in the treatment of S. typhi was tested and appeared that all the persisting isolates of S. typhi obtained from patients who suffered typhoid fever showed high resistance to gentamycin, ampicillin amikacin, and ceftriaxone; these results agree with Andualem et al and Alaarajy et al (11,12) they were found that all isolates were resistant to four or more classes of antibiotics as antimicrobials. The present study implemented to identifyWzx and Wzy genes in Salmonella typhi isolates that have an important role in typhoid diagnosis via O-antigen, although of the low sensitivity and specificity of Widal test in typhoid diagnosis, it is still used in some labs (11), on the other hands the false negative results (13,14) may be associated with genetic diversity of O-antigen genes which formed diversity in O-antigen proteins. The evidence found that according to The Kauffmann-White scheme there are 46 somatic O antigens of Salmonella, thus the diversity of O-antigens encoded genes can be used in the PCR development test to molecular serotypes identification.…”
Section: Discussionmentioning
confidence: 99%
“…The present finding needs more investigation to detect the genetic diversity of O-antigen in S. typhi which may have an important role in serotypes identification and development of knew molecular tests for typhoid fever diagnosis. The detection of these genes in S. typhi was poor in the last decades, Yara et al (13) in a study dealing with the pangenome of Salmonella O-Antigens, they found high metabolic and genetic differences within and across O-antigen groups in Salmonella strains. In Iraq, there were high multi-resistance bacterial isolates in the last decade which contributed to the health problems of diseases infection and this need to focused efforts for developing a new method for diagnosis and treatments based on the new finding like O-antigen encoded genes.…”
Section: Discussionmentioning
confidence: 99%