Molecular Mechanism and Agricultural Application of the NifA–NifL System for Nitrogen Fixation

Zhang, Wenyao; Chen, Yihang; Huang, Keyang; Wang, Feng; Mei, Ziqing

doi:10.3390/ijms24020907

Cited by 23 publications

(12 citation statements)

References 100 publications

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“…The combination of P. fluorescens with inorganic and organic fertilizers did increase the nitrogen content of reclaimed soil in coal-mining areas. This is because P. fluorescens N137-1 and N64-1 are autotrophic nitrogen-fixing bacteria that can convert N 2 from the atmosphere into ammonia and store it in the soil [ 32 ], although the nitrogen fixation ability of P. fluorescens is not as good as that of symbiotic and combined nitrogen-fixing bacteria such as rhizobia and nitrogen-fixing spirochetes, it has advantages such as low host specificity, wide distribution, and strong adaptability, and they are more conducive to colonization and function in coal-mining reclamation areas with harsh environments. Moreover, P. fluorescens can directly reduce the pH value of the surrounding soil by synthesizing organic acids such as indole-3-acetic acid [ 33 ], reducing the loss of NH 4 + -N caused by acid–base reactions.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas fluorescens with Nitrogen-Fixing Function Facilitates Nitrogen Recovery in Reclaimed Coal Mining Soils

Wu,

Wang,

Meng

et al. 2023

Microorganisms

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Coal mining has caused significant soil nitrogen loss in mining areas, limiting reclamation and reuse in agriculture. This article studies the effects of organic fertilizer, inorganic fertilizer, and the combined application of Pseudomonas fluorescens with the ability of nitrogen fixation on soil nitrogen accumulation and composition in the reclamation area of the Tunlan Coal Mine from 2016 to 2022 under the conditions of equal nitrogen application, providing a scientific basis for microbial fertilization and the rapid increase in nitrogen content in the reclaimed soil of mining areas. The results showed that as the reclamation time increased, the nitrogen content and the composition and structure of the soil treated with fertilization rapidly evolved toward normal farmland soil. The soil nitrogen content increased most rapidly in the presence of added P. fluorescens + organic fertilizer (MB). Compared to other treatments (inorganic fertilizer (CF), organic fertilizer (M), and P. fluorescens + inorganic fertilizer (CFB)), MB increased total nitrogen (TN) to normal farmland soil levels 1–3 years earlier. The comprehensive scores of MB and CFB on the two principal components increased by 1.58 and 0.79 compared to those of M and CF treatments, respectively. This indicates that the combination of P. fluorescens and organic fertilizer improves soil nitrogen accumulation more effectively than the combination of P. fluorescens and inorganic fertilizer. In addition, the application of P. fluorescens increases the content of unknown nitrogen (UN) in acid-hydrolysable nitrogen (AHN) and decreases the content of amino acid nitrogen (AAN) and ammonia nitrogen (AN). However, there was no significant effect on the content of ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3−-N) in soil-mineralized nitrogen (SMN). When combined with inorganic fertilizer, the contribution of SMN to TN increased by 14.78%, while when combined with organic fertilizer, the contribution of AHN to TN increased by 44.77%. In summary, the use of P. fluorescens is beneficial for nitrogen recovery in the reclaimed soil of coal-mining areas. The optimal fertilization method under the experimental conditions is the combination of P. fluorescens and organic fertilizer.

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Section: Discussionmentioning

confidence: 99%

Pseudomonas fluorescens with Nitrogen-Fixing Function Facilitates Nitrogen Recovery in Reclaimed Coal Mining Soils

Wu,

Wang,

Meng

et al. 2023

Microorganisms

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“…In addition to rhizobacteria, fungal groups that play a key role in agricultural productivity include Aspergillus , Fusarium , Penicillium , Piriformospora , Phoma and Trichoderma ( Hossain et al, 2017 ). Of all the interactions between plants and microorganisms in the rhizosphere, biological nitrogen fixation is by far the most researched ( Chen et al, 2019 ; Li et al, 2019 ; Soumare et al, 2020 ; Aasfar et al, 2021 ; Klimasmith and Kent, 2022 ; Zhang et al, 2023 ). Nitrogen-fixing bacteria like Rhizobia make unavailable N 2 accessible to plants.…”

Section: Plant-associated Microorganisms In Agriculturementioning

confidence: 99%

Nanoparticle applications in agriculture: overview and response of plant-associated microorganisms

Mgadi,

Ndaba,

Roopnarain

et al. 2024

Front. Microbiol.

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Globally, food security has become a critical concern due to the rise in human population and the current climate change crisis. Usage of conventional agrochemicals to maximize crop yields has resulted in the degradation of fertile soil, environmental pollution as well as human and agroecosystem health risks. Nanotechnology in agriculture is a fast-emerging and new area of research explored to improve crop productivity and nutrient-use efficiency using nano-sized agrochemicals at lower doses than conventional agrochemicals. Nanoparticles in agriculture are applied as nanofertilizers and/or nanopesticides. Positive results have been observed in terms of plant growth when using nano-based agricultural amendments. However, their continuous application may have adverse effects on plant-associated rhizospheric and endospheric microorganisms which often play a crucial role in plant growth, nutrient uptake, and disease prevention. While research shows that the application of nanoparticles has the potential to improve plant growth and yield, their effect on the diversity and function of plant-associated microorganisms remains under-explored. This review provides an overview of plant-associated microorganisms and their functions. Additionally, it highlights the response of plant-associated microorganisms to nanoparticle application and provides insight into areas of research required to promote sustainable and precision agricultural practices that incorporate nanofertilizers and nanopesticides.

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“…The green biotechnology in agriculture is based on the use of microalgae biomass as soil biofertilizer for crop cultivation and phytoremediation, reviewed in [170]. Furthermore, there is increasing interest in the use of N-fixing bacteria as a biofertilizer for crops [18]. Free-living aerobic diazotroph like Azotobacter spp.…”

Section: Biotechnological Potentialmentioning

confidence: 99%

“…BNF is a highly regulated process due to the high energetic demands of the main enzyme involved in this reaction, nitrogenase. Nitrogenase requires 16 moles of ATP and 8 moles of electrons to reduce one mole of N 2 to ammonium [18]. Several organisms rely directly or indirectly on diazotrophs and establish symbiotic interactions with them for proper growth.…”

Section: Introductionmentioning

confidence: 99%

Microalgal and Nitrogen-Fixing Bacterial Consortia: From Interaction to Biotechnological Potential

Llamas,

Leon-Miranda,

Tejada-Jimenez

2023

Plants

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Microalgae are used in various biotechnological processes, such as biofuel production due to their high biomass yields, agriculture as biofertilizers, production of high-value-added products, decontamination of wastewater, or as biological models for carbon sequestration. The number of these biotechnological applications is increasing, and as such, any advances that contribute to reducing costs and increasing economic profitability can have a significant impact. Nitrogen fixing organisms, often called diazotroph, also have great biotechnological potential, mainly in agriculture as an alternative to chemical fertilizers. Microbial consortia typically perform more complex tasks than monocultures and can execute functions that are challenging or even impossible for individual strains or species. Interestingly, microalgae and diazotrophic organisms are capable to embrace different types of symbiotic associations. Certain corals and lichens exhibit this symbiotic relationship in nature, which enhances their fitness. However, this relationship can also be artificially created in laboratory conditions with the objective of enhancing some of the biotechnological processes that each organism carries out independently. As a result, the utilization of microalgae and diazotrophic organisms in consortia is garnering significant interest as a potential alternative for reducing production costs and increasing yields of microalgae biomass, as well as for producing derived products and serving biotechnological purposes. This review makes an effort to examine the associations of microalgae and diazotrophic organisms, with the aim of highlighting the potential of these associations in improving various biotechnological processes.

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Molecular Mechanism and Agricultural Application of the NifA–NifL System for Nitrogen Fixation

Cited by 23 publications

References 100 publications

Pseudomonas fluorescens with Nitrogen-Fixing Function Facilitates Nitrogen Recovery in Reclaimed Coal Mining Soils

Pseudomonas fluorescens with Nitrogen-Fixing Function Facilitates Nitrogen Recovery in Reclaimed Coal Mining Soils

Nanoparticle applications in agriculture: overview and response of plant-associated microorganisms

Microalgal and Nitrogen-Fixing Bacterial Consortia: From Interaction to Biotechnological Potential

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