Molecular mechanism and targeted therapy of Hsp90 involved in lung cancer: New discoveries and developments (Review)

Biaoxue, Rong; Yang, Shuanying

doi:10.3892/ijo.2017.4214

Cited by 46 publications

(41 citation statements)

References 101 publications

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“…Additionally, the expression patterns of both HSP90 and ENO1 were found uniformly increased across all UM-EVs. HSP90 is a molecular chaperone reported to be of crucial importance in cancer cell growth and survival owing to its involvement in promoting the MAPK and P13K/AKT pathways [ 80 , 81 ]. In similar fashion, ENO1 is linked to the AKT signaling pathway, is involved in promoting gastric cancer cell proliferation and metastasis and serves as a potential biomarker for certain cancers [ 72 , 74 ].…”

Section: Discussionmentioning

confidence: 99%

Uveal Melanoma-Derived Extracellular Vesicles Display Transforming Potential and Carry Protein Cargo Involved in Metastatic Niche Preparation

Tsering

Laskaris

Abdouh

et al. 2020

Cancers

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Extracellular vesicles (EVs) carry molecules derived from donor cells and are able to alter the properties of recipient cells. They are important players during the genesis and progression of tumors. Uveal melanoma (UM) is the most common primary intraocular tumor in adults and is associated with a high rate of metastasis, primarily to the liver. However, the mechanisms underlying this process are poorly understood. In the present study, we analyzed the oncogenic potential of UM-derived EVs and their protein signature. We isolated and characterized EVs from five UM cell lines and from normal choroidal melanocytes (NCMs). BRCA1-deficient fibroblasts (Fibro-BKO) were exposed to the EVs and analyzed for their growth in vitro and their reprograming potential in vivo following inoculation into NOD-SCID mice. Mass spectrometry of proteins from UM-EVs and NCM-EVs was performed to determine a protein signature that could elucidate potential key players in UM progression. In-depth analyses showed the presence of exosomal markers, and proteins involved in cell-cell and focal adhesion, endocytosis, and PI3K-Akt signaling pathway. Notably, we observed high expression levels of HSP90, HSP70 and integrin V in UM-EVs. Our data bring new evidence on the involvement of UM-EVs in cancer progression and metastasis.

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Section: Discussionmentioning

confidence: 99%

Uveal Melanoma-Derived Extracellular Vesicles Display Transforming Potential and Carry Protein Cargo Involved in Metastatic Niche Preparation

Tsering

Laskaris

Abdouh

et al. 2020

Cancers

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“…The development of target small molecule inhibitors provides an alternative treatment strategy to chemotherapy for lung cancer patients, with improved survival rate and life quality [ 2 , 3 ]. The exploration of the molecular mechanisms in lung cancer is very important for developing new strategies of diagnosis and treatment to this disease [ 4 ]. Novel therapeutic targets together with prognostic biomarkers for lung carcinogenesis have been discovered due to the development of molecular biology techniques [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Hsa_circ_0046264 up-regulated BRCA2 to suppress lung cancer through targeting hsa-miR-1245

et al. 2018

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ObjectiveLung cancer had been leading mounts of deaths worldwide. Advances in genes microarray had helped human further understand genes and identify novel circular RNAs. This study aimed at investigating the biological functions and molecular mechanisms of hsa_circ_0046264 in lung cancer which may be helpful in lung cancer early diagnosis and clinical treatment.MethodsGene microarray data screened the differential gene of hsa_circ_0046264 and its downstream genes were found by bioinformatics analysis and verified by luciferase reporter assay. QRT-PCR and Western blot was used to detect the RNA and protein levels respectively. RNase R digestion confirmed the existences of circular RNA. Cell viability, invasion and apoptosis were determined by MTT assay, flow cytometry and DNA damage assay. Tumor formation in nude mice and immunohistochemistry proved the functions of hsa_circ_0046264 in vivo.ResultsHsa_circ_0046264 and BRCA2 were down-regulated in lung cancer tissues while miR-1245 was up-regulated. Hsa_circ_0046264 induced apoptosis but inhibited proliferation and invasion of lung cancer cells through targeting miR-1245 to up-regulate BRCA2. Hsa_circ_0046264 inhibited the tumor growth in vivo.ConclusionHsa_circ_0046264 was a tumor suppressor in lung cancer. Overexpression of hsa_circ_0046264 could up-regulate BRCA2 expression through down-regulating of miR-1245.Electronic supplementary materialThe online version of this article (10.1186/s12931-018-0819-7) contains supplementary material, which is available to authorized users.

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“…Sourbier [30] et al systematically explored the significance of HSP90α as a potential biomarker in liver cancer in their latest study. While, Zhang [31] Staging groups compared and pairwise comparisons…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of HSP90α and Related Markers in Lung Cancer

Yuan¹,

Hong²,

Li³

et al. 2019

Preprint

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Aims To prove the expression of heat shock protein 90α (HSP90α) in the lung cancer and the clinical value of HSP90α and related markers in the diagnosis of lung cancer. Methods The concentrations of HSP90α and related markers were detected in the blood of 560 lung cancer patients by enzyme-linked immunosorbent assay for analyzing the statistical differences of HSP90α between the patients group and the healthy group in patients' age, gender, different pathological types, lung cancer staging, and metastasis status, as well as the differences and evaluate the value of HSP90α and related markers in lung cancer diagnosis. Results The results showed no statistical difference in HSP90α among different age groups. And the HSP90α level cannot be distinguished by genders significantly (P>0.05); In the group by lung cancer type, statistical differences were found in the HSP90α level between the small cell lung cancer group and the squamous cell carcinoma group (P<0.05); In the group by stage, the HSP90α level of high staging was significantly higher than that low staging (P<0.05), and the significant difference among the groups; the HSP90α level at I/II/III/IV shows statistical differences among the groups (P<0.05); And the test result of HSP90α was higher in the metastatic group than in the non-metastatic group significantly, and the significant difference between the two groups (P<0.05). The r value of the HSP90α and related markers in the diagnosis of lung cancer: NSE>CEA>ProGRP>CF211 (P<0.05). While HSP90α and related markers didn’t fit the satisfactory conformance, in terms of the positive rate of diagnosis, there were statistically differences in the diagnostic positive rate between HSP90α and each maker (P<0.01). Reducing HSP90α clinical references in lung cancer combined diagnosis can effectively improve the positive rate of the combined diagnosis. Conclusion HSP90α has significant value on early screening and diagnosis of lung cancer. The combined application of HSP90α and related markers can improve the positive rate of early diagnosis of lung cancer effectively.

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Molecular mechanism and targeted therapy of Hsp90 involved in lung cancer: New discoveries and developments (Review)

Cited by 46 publications

References 101 publications

Uveal Melanoma-Derived Extracellular Vesicles Display Transforming Potential and Carry Protein Cargo Involved in Metastatic Niche Preparation

Uveal Melanoma-Derived Extracellular Vesicles Display Transforming Potential and Carry Protein Cargo Involved in Metastatic Niche Preparation

Hsa_circ_0046264 up-regulated BRCA2 to suppress lung cancer through targeting hsa-miR-1245

Diagnostic value of HSP90α and Related Markers in Lung Cancer

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