Molecular mechanism of cell cycle blockage of hepatoma SK-Hep-1 cells by Epimedin C through suppression of mitogen-activated protein kinase activation and increased expression of CDK inhibitors p21Cip1 and p27Kip1

Ultrasonic-assisted extraction of epimedin C from fresh leaves of Epimedium and extraction mechanism

“…1 shows that the extraction yield of epimedin C decreases in the order: CH 3 OH N C 2 H 5 OH N H 2 O N CH 3 CN. Moreover, it was reported that epimedin C isolated from the methanolic extract displayed immunomodulatory effect and possessed anti-tumor activity (Iinuma et al, 1990;Liu et al, 2006). Hence, methanol was chosen as the best solvent in the following extraction experiments.…”

Liquid chromatography–tandem mass spectrometry analysis of metabolites in rats after administration of prenylflavonoids from Epimediums

“…Meanwhile, as the principal prenylflavonoid in Epimedium wushanense, epimedin C enhanced the response of spleen antibodyforming cells significantly [8]. Recent study also revealed its anti-tumor activity against hepatoma SK-Hep-1 cells [9].…”

Induction of Apoptosis by Icariside II through Extrinsic and Intrinsic Signaling Pathways in Human Breast Cancer MCF7 Cells

“…18) There have also been several reports of other prenylflavonoids derived from Epimedium as an anticancer agent to MCF7 cells, 19,20) Hec1A cells, 21) PC-3 cells, 22) HEPG2 cells, 23,24) SMMC-7721 cells, 25) and SK-Hep-1 cells. 26) However, the paucity of literature y To whom correspondence should be addressed. Xiaoguang CHEN, Tel: +86-10-63165207; Fax: +86-10-63017757; E-mail: chxg@imm.ac.cn; Baolin GUO, Tel: +86-10-57833172; Fax: +86-10-57833288; E-mail: guobaolin010@163.com Abbreviations: AIF, apoptosis-inducing factor; BPB, bromophenol blue; PVDF, polyvinylidene fluoride; PI, propidium iodide; DAPI, 4 0 ,6-diamidino-2-phenylindole; JC-1, 5,5 0 ,6,6 0 -tetra-chloro-1,1 0 ,3,3 0 -tetra-ethylbenzimidazolyl-carbocyanine iodide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PARP, poly (ADP-ribose) polymerase; TNF, tumor necrosis factor; TRAIL, TNF-related apoptosis-inducing ligand; PAGE, polyacrylamide gel electrophoresis about the IcaS role in breast cancer prompted us to investigate this, and we observed that IcaS inhibited the proliferation of both MCF7 and MDA-MB-231 cells.…”