Molecular Mechanism of Chloramphenicol and Thiamphenicol Resistance Mediated by a Novel Oxidase, CmO, in
            <i>Sphingomonadaceae</i>

Ma, Xiaodan; Zhang, Liying; Ren, Yijun; Yun, Hui; Cui, Hanlin; Li, Qian; Guo, Yuanqiang; Gao, Shuhong; Zhang, Feng‐Liang; Wang, Aijie; Liang, Bin

doi:10.1128/aem.01547-22

Cited by 9 publications

(3 citation statements)

References 64 publications

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“…CAP‐1 and oxidoreductase CapO from Sphingomonas sp. CL5.1, act on the C 3 ‐OH of the CHL side chain (Ma et al, 2022; Zhang et al, 2022). The reductases ChlOR QY071 , ChlOR L‐11A and ChlOR LMS , which share a relatively low amino acid sequence identity (<30%) with the reported CHL‐inactivating enzymes, are present in the gram‐positive bacterium Nocardioides sp.…”

Section: Discussionmentioning

confidence: 99%

“…CAP‐1 and Sphingopyxis sp. GC21 were reported (Ma et al, 2022; Zhang et al, 2022). CapO can oxidize CHL to the corresponding aldehyde, and subsequently to the corresponding carboxylic acid (Zhang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…The side chain of CHL could be oxygenated by CmO mainly to oxidized chloramphenicol (O‐CHL) and little to PNBD. These two products could be degraded to PNBA by strain CL5.1 (Ma et al, 2022). However, in their study, the enzymes involved in the conversion of O‐CHL to PNBA or PNBD to PNBA were not identified.…”

Section: Introductionmentioning

confidence: 99%

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ChlOR, a GMC family oxidoreductase that evolved independently from the actinomycete, confers resistance to amphenicol antibiotics

Qian,

Cheng,

Lai

et al. 2023

Environmental Microbiology

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Overuse of the amphenicol antibiotics chloramphenicol (CHL) and thiamphenicol (TAP) poses a great threat to ecosystem safety and human health. The strain, Nocardioides sp. LMS‐CY, Nocardioides sp. QY071 and Nocardioides sp. L‐11A, classified as a gram‐positive actinomycete, harbours a complete CHL metabolic pathway. However, the metabolic genes (clusters) involved in the entire pathway in gram‐positive actinomycetes are still limited. Here, chlORLMS, chlORQY071 and chlORL‐11A completely from the actinomycete Nocardioides spp. were found to act on the C1‐OH of the CHL/TAP side chain, directly converting CHL/TAP to 4‐nitrobenzaldehyde (PNBD)/4‐methylsulfonyl benzaldehyde (PMBD) and transforming PNBD/PMBD into 4‐nitrobenzyl alcohol (PNBM)/4‐methylsulfonyl phenyl methanol (PMBM). Furthermore, oxidoreductases can transform PNBM into 4‐nitrobenzoate (PNBA). The oxidoreductases ChlORLMS, ChlORQY071 and ChlORL‐11A were all classified as cellobiose dehydrogenases from the glucose methanol choline (GMC) family. Based on the Swiss‐Prot database, ChlORQY071 exhibited a lower identity (27.12%–35.10% similarity) with the reported oxidoreductases. Enzymatic and molecular docking analyses showed that ChlORQY071 and ChlORL‐11A from the two similar genomes were remarkably more effective in metabolizing CHL than ChlORLMS. Overall, the detailed resistance mechanism of CHL/TAP by actinomycete strains isolated from soil and livestock manure will provide insights into the occurrence of CHL/TAP resistance genes in the environment, resistance risk and bioremediation of CHL/TAP‐contaminated environments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ChlOR, a GMC family oxidoreductase that evolved independently from the actinomycete, confers resistance to amphenicol antibiotics

Qian,

Cheng,

Lai

et al. 2023

Environmental Microbiology

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Antibiotic Resistance Dissemination and Mapping in the Environment Through Surveillance of Wastewater

Raju,

Ansari,

Patil

et al. 2024

Journal of Basic Microbiology

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Antibiotic resistance is one of the major health threat for humans, animals, and the environment, according to the World Health Organization (WHO) and the Global Antibiotic‐Resistance Surveillance System (GLASS). In the last several years, wastewater/sewage has been identified as potential hotspots for the dissemination of antibiotic resistance and transfer of resistance genes. However, systematic approaches for mapping the antibiotic resistance situation in sewage are limited and underdeveloped. The present review has highlighted all possible perspectives by which the dynamics of ARBs/ARGs in the environment may be tracked, quantified and assessed spatio‐temporally through surveillance of wastewater. Moreover, application of advanced methods like wastewater metagenomics for determining the community distribution of resistance at large has appeared to be promising. In addition, monitoring wastewater for antibiotic pollution at various levels, may serve as an early warning system and enable policymakers to take timely measures and build infrastructure to mitigate health crises. Thus, by understanding the alarming presence of antibiotic resistance in wastewater, effective action plans may be developed to address this global health challenge and its associated environmental risks.

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Bacterial and genetic resources for typical emerging pharmaceuticals and personal care products degradation

Yun,

Wang,

et al. 2024

Water Security: Big Data-Driven Risk Identification, Assessment and Control of Emerging Contaminants

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Molecular Mechanism of Chloramphenicol and Thiamphenicol Resistance Mediated by a Novel Oxidase, CmO, in Sphingomonadaceae

Cited by 9 publications

References 64 publications

ChlOR, a GMC family oxidoreductase that evolved independently from the actinomycete, confers resistance to amphenicol antibiotics

ChlOR, a GMC family oxidoreductase that evolved independently from the actinomycete, confers resistance to amphenicol antibiotics

Antibiotic Resistance Dissemination and Mapping in the Environment Through Surveillance of Wastewater

Bacterial and genetic resources for typical emerging pharmaceuticals and personal care products degradation

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