Molecular Mechanism of Circ_0088300–BOLL Interaction Regulating Mitochondrial Metabolic Reprogramming and Involved in Gastric Cancer Growth and Metastasis

Chu, Songtao; Fei, Bingyuan; Yu, Miao

doi:10.1021/acs.jproteome.3c00476

Cited by 3 publications

(3 citation statements)

References 51 publications

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“…The ceRNA mechanism, where circRNAs act as sponges for miRNAs, modulating mRNA expression, has been extensively studied in cancer biology. [22][23][24] .…”

Section: Discussionmentioning

confidence: 99%

The study of competing endogenous RNAs regulatory network in hepatocellular carcinoma via bioinformatics

Huang,

Zhao,

Mao

et al. 2024

Preprint

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Background Hepatocellular carcinoma (HCC) is among the most prevalent and lethal cancers globally and individuals diagnosed at advanced stages. The discovery of new diagnostic and prognostic markers in HCC is urgent. Circular RNAs (circRNAs) have emerged as key players in the intricate landscape of gene regulation through the competing endogenous RNA (ceRNA) mechanism. However, the ceRNA mechanism of circRNAs in HCC still remains unclear. Methods This study conducted a comprehensive HCC analysis using GEO database expression profiles for circRNAs, miRNAs, and mRNAs. Differentially expressed genes (DEGs) were revealed and visually presented through R-generated volcano plots and heatmaps. The STRING website and Cytoscape facilitated the construction of a protein-protein interaction (PPI) network. Functional enrichment analyses validated signaling pathways, and a circRNA-miRNA-mRNA network was constructed through Cytoscape. Results The study identified 86 differentially expressed mRNAs (33 upregulated, 43 downregulated) across GSE168852, GSE169289, and GSE202069 datasets. Volcano plots and Venn diagrams illustrated gene expression changes. Gene ontology (GO) and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis revealed functional insights. A PPI network identified 8 key genes (HMMR, EXO1, TOP2A, CCNB1, NUF2, CCNB2, BUB1, BUB1B) validated by GEPIA and Kaplan-Meier Plotter. The Cytoscape built ceRNA network unveiled regulatory modules involving 4 mRNAs, 9 miRNAs, and 31 circRNAs. Conclusions In summary, this study established circRNA-miRNA-mRNA regulatory modules, including 4 mRNAs, 9 miRNAs, and 31 circRNAs. This offers an effective bioinformatics strategy for studying HCC molecular mechanisms and prognosis. This might provide a realm of the molecular with diagnosis and prognosis biomarkers in HCC.

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“…The ceRNA mechanism, where circRNAs act as sponges for miRNAs, modulating mRNA expression, has been extensively studied in cancer biology. [22][23][24] .…”

Section: Discussionmentioning

confidence: 99%

The study of competing endogenous RNAs regulatory network in hepatocellular carcinoma via bioinformatics

Huang,

Zhao,

Mao

et al. 2024

Preprint

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“…They act as miRNA sponges by competitively binding to miRNAs, leading to the derepression of miRNAs on the target mRNAs [ 17 ]. Additionally, they function as transcriptional and splicing regulators through binding to RNA-binding proteins [ 18 ]. Intriguingly, some circRNAs can serve as templates for polypeptide synthesis [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Exosome-transported circ_0061407 and circ_0008103 play a tumour-repressive role and show diagnostic value in non-small-cell lung cancer

Chen,

Ma,

Chen

et al. 2024

J Transl Med

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Background Circular RNAs (circRNAs), one of the major contents of exosomes, have been shown to participate in the occurrence and progression of cancers. The role and the diagnostic potential of exosome-transported circRNAs in non-small-cell lung cancer (NSCLC) remain largely unknown. Methods The NSCLC-associated exosomal circ_0061407 and circ_0008103 were screened by circRNA microarray. The role of circ_0061407 and circ_0008103 in NSCLC was examined in vitro and in vivo. The encapsulation of the two circRNAs into exosomes and the transport to recipient cells were observed by confocal microscopy. The effects of exosome-transported circ_0061407 and circ_0008103 on recipient cells were investigated using a co-culture device. Bioinformatics analyses were performed to predict the mechanisms by which circ_0061407 and circ_0008103 affected NSCLC. The quantitative polymerase chain reaction was used to quantify the exosome-containing circ_0061407 and circ_0008103 in the serum samples of healthy, pneumonia, benign lung tumours, and NSCLC. The diagnostic efficacy was evaluated using receiver operating characteristic curves. Results The levels of circ_0061407 and circ_0008103 within exosomes were down-regulated in the serum of patients with NSCLC. The up-regulation of circ_0061407 and circ_0008103 inhibited the proliferation, migration/invasion, cloning formation of NSCLC cells in vitro and inhibited lung tumour growth in vivo. Circ_0061407 and circ_0008103 were observed to be packaged in exosomes and transported to recipient cells, where they inhibited the proliferation, migration/invasion, and cloning formation abilities of the recipient cells. Moreover, circ_0061407 and circ_0008103 might be involved in the progression of NSCLC by interacting with microRNAs and proteins. Additionally, lower serum exosomal circ_0061407 and circ_0008103 levels were associated with advanced pathological staging and distant metastasis. Conclusions This study identified two novel exosome-transported circRNAs (circ_0061407 and circ_0008103) associated with NSCLC. These findings may provide additional insights into the development of NSCLC and potential diagnostic biomarkers for NSCLC.

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“…Noncoding RNAs (ncRNAs) play important roles in different types of cancers, including microRNAs, long noncoding RNAs, and circular RNAs. − MicroRNAs, which attach to the 3′ untranslated region (UTR) of mRNA and either inhibit its translation or promote its degradation, are a type of short (18–22 nt), single-stranded, noncoding RNA molecules that are evolutionarily conserved. , Importantly, a single miRNA can exert repressive effects on hundreds of genes that might be regulated in a variety of cellular activities, including adhesion, migration, apoptosis, cellular differentiation, proliferation, and control of the cell cycle. , Since their discovery, miRNAs have been found to have significant implications for tumorigenesis and tumor progression, including CC. , Mature miR-138–5p originates from two intergenic loci located on chromosomes 3 (3p21) and 16 (16q13), known as miR-138–1 and miR-138–2, respectively . MiR-138–5p acts as a tumor suppressor and is dramatically downregulated in various cancers, such as colorectal, prostate, gastric, liver, and cervical cancers .…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Reveal the Mechanism of MiR-138–5p Suppressing Cervical Cancer via Targeting ZNF385A

Peng,

Deng,

et al. 2024

J. Proteome Res.

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MicroRNAs are short, noncoding RNA molecules that exert pivotal roles in cancer development and progression by modulating various target genes. There is growing evidence that miR-138−5p is significantly involved in cervical cancer (CC). However, its precise molecular mechanism has yet to be fully understood. In the current investigation, a quantitative proteomics approach was utilized to detect possible miR-138−5p targets in HeLa cells systematically. In total, 364 proteins were downregulated, and 150 were upregulated after miR-138−5p overexpression. Bioinformatic analysis of these differentially expressed proteins (DEPs) revealed significant enrichment in several cancerrelated pathways. Zinc finger protein 385A (ZNF385A) was determined as a novel direct target of miR-138−5p and discovered to facilitate the proliferation, migration, and cell cycle progression of HeLa cells. SFN and Fas cell surface death receptor(FAS) were then identified as functional downstream effectors of ZNF385A and miR-138−5p. Moreover, a tumor xenograft experiment was conducted to validate the association of miR-138−5p-ZNF385A-SFN/FAS axis with the development of CC in vivo. Our findings have collectively established a catalog of proteins mediated by miR-138−5p and have provided an in-depth comprehension of the molecular mechanisms responsible for the inhibitory effect of miR-138−5p on CC. The miR-138−5p-ZNF385A-SFN/FAS axis could also be beneficial to the identification of new therapeutic targets.

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Molecular Mechanism of Circ_0088300–BOLL Interaction Regulating Mitochondrial Metabolic Reprogramming and Involved in Gastric Cancer Growth and Metastasis

Cited by 3 publications

References 51 publications

The study of competing endogenous RNAs regulatory network in hepatocellular carcinoma via bioinformatics

The study of competing endogenous RNAs regulatory network in hepatocellular carcinoma via bioinformatics

Exosome-transported circ_0061407 and circ_0008103 play a tumour-repressive role and show diagnostic value in non-small-cell lung cancer

Quantitative Proteomics Reveal the Mechanism of MiR-138–5p Suppressing Cervical Cancer via Targeting ZNF385A

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