Molecular mechanism of Copper Albumin Complex on NDEA induced brain vascular damage via promoting VEGF expression.

Abstract

Nitrosodiethylamine (NDEA) is a potent oxidant induces neurodegeneration via (reactive oxygen species) ROS. Copper is an important metal essential for scavenging free radicals, development of central nervous system (CNS) and redox angiogenesis signaling. Vascular endothelial growth factor (VEGF) is well known as efficacious and long-term signal that stimulates angiogenesis, where its expression is copper dependent. We examined the copper protective effect against brain vascular damage initiated by NDEA. NDEA induces brain vascular wall damage, necrosis with interstitial hemorrhage and diminishes VEGF expression. Histopathological examination showing a great improvement of brain tissue in copper treated mice with significant increase in VEGF expression. Higher levels of intracellular copper can stimulate angiogenesis and exhibited a significant protection against NDEA induced brain vascular damage, confirming its ability to enhance antioxidant activity and angiogenesis initiation. Our report presents first evidence that inducible VEGF expression in brain is sensitive to copper; moreover, copper-based therapeutics represents a novel approach to reduce brain vascular damage induced by NDEA generated ROS.

Investigation of the Ameliorating Effect of Copper Albumin Complex on Lysyl oxidase in monosodium iodoacetate -Induced Knee Osteoarthritis in Rats

“…Positive control: included on 20 osteoarthritic rats which left without treatment and were divided equally into mild and severe not treated subgroups according to the detected clinical signs which are pain, swelling and, lameness. Treated group: included on 20 osteoarthritic rats which were divided equally into mild and severe treated groups according to severity of clinical signs and treated orally with copper albumin complex (obtained from Prof. Dr. Ahmed Yassein Nassar, professor of biochemistry, faculty of Medicine, Assiut University, Assiut, Egypt as patent cooperation treaty (PCT) in the international Bureau of World Intellectual Property Organization (WIPO), Geneva, Switzerland / World Organization (WO) 2008 / 028497) for a month at a dose of 1 ml/kg B.W daily after suspension of 817μg/kg copper albumin complex in water (Taha et al, 2022).…”

In Vivo Investigation of the Ameliorating Effect of Copper Albumin Complex on chondroitin sulfate in Monosodium iodoacetate -Induced Knee Osteoarthritis

“…C-Twenty treated rats' group, where the rats were divided into mild and severe treated groups (10 rats each) based on the severity of their symptoms. After dissolving 817μg/kg of copper albumin complex in water, the rats were given copper albumin complex orally once daily for a month at a dose of 1 ml/kg B.W (Taha et al, 2022).…”