Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer

Nan, Zheng; Liu, Shan; Zeng, Huiting; Zhao, Huajun; Jin, Lixu

doi:10.3390/jcm11237220

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…The role of curcumin as a cancer chemopreventive agent has been rigorously studied in various cancer models. [20][21][22][23][24] Recent studies have highlighted curcumin's antiproliferative and antimotility effects on breast cancer cells, yet the intricate molecular mechanisms underlying these actions require further elucidation. [25][26][27] Therefore, exploring curcumin's influence on cell motility, proliferation, cell cycle, and apoptosis, particularly in TNBC, and uncovering its molecular underpinnings are of paramount clinical relevance.…”

Section: Discussionmentioning

confidence: 99%

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Chen,

Lu,

et al. 2024

Medicine

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This study aimed to use bioinformatics approaches for predicting the anticancer mechanisms of curcumin on triple-negative breast cancer (TNBC) and to verify these predictions through in vitro experiments. Initially, the Cell Counting Kit-8 (CCK8) assay was employed to rigorously investigate the influence of curcumin on the proliferative capacity of TNBC cells. Subsequently, flow cytometry was employed to meticulously assess the impact of curcumin on cellular apoptosis and the cell cycle regulation. Transwell assays were employed to meticulously evaluate the effect of curcumin on the motility of TNBC cells. RNA sequencing was conducted, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of differentially expressed genes, aiming to elucidate the potential anticancer mechanisms underlying curcumin’s effects. To thoroughly elucidate the interactions among multiple proteins, we constructed a protein–protein interaction (PPI) network. Finally, the expression levels of several key proteins, including fibronectin, mTOR, β-Catenin, p-Akt, Akt, N-Cadherin, p-S6, and S6, were assessed using the western blot. The CCK8 assay results showed that curcumin significantly inhibited the proliferation of Hs578T and MDA-MB-231 cells. Flow cytometry results showed that curcumin induced apoptosis in these cells and arrested the cell cycle at the G2/M phase. Additionally, Transwell assay results showed that curcumin effectively reduced the motility of Hs578T and MDA-MB-231 cells. Enrichment analysis of RNA sequencing data showed that the mechanism of action of curcumin was significantly associated with signaling pathways such as pathways in cancer, focal adhesion, and PI3K-Akt signaling pathways. Subsequently, we constructed a protein–protein interaction network to elucidate the interactions among multiple proteins. Finally, Western blotting analysis showed that curcumin significantly decreased the expression levels of key proteins including Fibronectin, mTOR, β-Catenin, p-Akt, Akt, N-Cadherin, p-S6, and S6. Curcumin exhibits its therapeutic potential in TNBC by modulating multiple signaling pathways. It may inhibit the epithelial-mesenchymal transition process by downregulating the expression of proteins involved in the mTOR and PI3K-Akt signaling pathways, thereby suppressing the motility of TNBC cells. These findings provide experimental evidence for considering curcumin as a potential therapeutic strategy in the treatment of TNBC.

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Section: Discussionmentioning

confidence: 99%

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Chen,

Lu,

et al. 2024

Medicine

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“…It exerts its anticancer effects by modulating multiple targets, such as negative regulation of tumor metastasis, angiogenesis, apoptosis, cell cycle progression, in ammation, and multidrug resistance pathways. It has been intensively studied as a cancer chemopreventive agent in various cancer models [13][14][15][16][17]. A recent study found that curcumin has potential anti-proliferative and motility effects against breast cancer, and its speci c molecular mechanism remains to be further explored [18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Curcumin regulates cell proliferation and invasion of human triple-negative breast cancer Hs578T cells via the mTOR signaling pathway

Chen

Zhang

et al. 2023

Preprint

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Curcumin, a polyphenolic compound isolated from the herb turmeric rhizome, is of interest for its potent biological activity, and in vitro studies have shown that it has broad anticancer activity. We investigated the impact of curcumin on the proliferation, migration, and invasion of TNBC Hs578T cells and further explored the underlying mechanisms. It was confirmed that the proliferation and motility of curcumin-treated Hs578T cells were inhibited, and bioinformatic RNA sequencing techniques further complemented the results we obtained. Finally, the sequencing results were validated using western blot experiments. Thus, we confirmed that curcumin could inhibit the proliferation and cell motility of TNBC Hs578T cells through the mTOR signaling pathway and the migration and invasion of Hs578T cells through EMT, which provide a theoretical and experimental basis for the clinical treatment of TNBC with curcumin by complementing each other through multiple approaches.

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Curcumin: a natural organic component that plays a multi-faceted role in ovarian cancer

et al. 2023

J Ovarian Res

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Curcumin, a natural organic component obtained from Curcuma longa’s rhizomes, shows abundant anti-tumor, antioxidant and anti-inflammatory pharmacological activities, among others. Notably the anti-tumor activity has aroused widespread attention from scholars worldwide. Numerous studies have reported that curcumin can delay ovarian cancer (OC), increase its sensitivity to chemotherapy, and reduce chemotherapy drugs’ side effects. It has been shown considerable anticancer potential by promoting cell apoptosis, suppressing cell cycle progression, inducing autophagy, inhibiting tumor metastasis, and regulating enzyme activity. With an in-depth study of curcumin’s anti-OC mechanism, its clinical application will have broader prospects. This review summarizes the latest studies on curcumin’s anti-OC activities, and discusses the specific mechanism, hoping to provide references for further research and applications.

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Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer

Cited by 3 publications

References 19 publications

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Curcumin suppresses metastasis of triple-negative breast cancer cells by modulating EMT signaling pathways: An integrated study of bioinformatics analysis

Curcumin regulates cell proliferation and invasion of human triple-negative breast cancer Hs578T cells via the mTOR signaling pathway

Curcumin: a natural organic component that plays a multi-faceted role in ovarian cancer

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