Molecular mechanism of pathogenesis of dengue virus: Entry and fusion with target cell

Abstract

Dengue fever is one of the major health problems in India. Interaction with specific receptor(s) at the cell surface is one of the first events in the pathogenesis of Dengue virus. However, relatively little is known about these receptors. Cellular receptors in human monocytes and mouse neural cells are main target for the viral infection. The envelope protein of the virus (E-protein) plays important role in attachment of virus to target cells and their interaction with cellular receptors. The modulation of receptor gene(s) and/or protein(s) can be used as a method for interfering with virus entry and can thus become a new method for disease prevention. The receptors can be purified by affinity chromatography using E-protein as ligand. It has been reported that addition of highly sulfated heparan sulfate prevents E-protein binding to target cells suggesting that heparan sulfate is utilized by dengue envelope protein to bind to target cells.

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

“…The genome of Dengue virus was 11,000 bases and codes three structural proteins of capsid protein C, membrane protein M and envelop protein E. The envelop protein E is on the viral surface and important for the initial attachment of the viral particle to the host cell with several molecules that interact with the viral E protein (Chang 1997;Seema and Jain 2005). The E protein contains three domains I-III and one trans-membrane domain.…”

An Analysis of the Genomes of Dengue Virus Using Decision Tree and Apriori Algorithm

“…The virus targets cellular receptors in human monocytes. The host cell provides surface receptors, shows endocytic activity, and triggers signals for penetration [1]. The E glycoprotein is responsible for virion attachment to the receptor and fusion of the virus envelope with the target cell membrane.…”

“…The four subtypes have come into existence by evolving independently by adapting to peridomestic mosquito vectors and human reservoir hosts [1]. The subtypes of dengue virus have 60-80% homology amongst themselves.…”

Identification of Putative Vero Cell Protein(s) that Bind Specifically to Recombinant Envelope Protein of Dengue Virus Type 2

“…This is achieved by the fusion of the viral membrane with a cellular membrane [3,4]. The initial binding of dengue virus to target cells is mediated by binding of the envelope protein to a specific and unidentified cell surface receptor(s) [5].…”