Molecular mechanism of plant elicitor daphnetin-carboxymethyl chitosan nanoparticles against Ralstonia solanacearum by activating plant system resistance

Wang, Yao; Yang, Liang; Zhou, Xiaofang; Wang, Ye; Liang, Yijia; Luo, Binshao; Dai, Yeling; Wei, Zhouling; Li, Shili; He, Rong; Ding, Wei

doi:10.1016/j.ijbiomac.2023.124580

Cited by 13 publications

(7 citation statements)

References 54 publications

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“…49 After treated with chitosan nanoparticles, the expressions of defense enzymes and PR proteins were significantly induced upon Ralstonia solanacearum infection in tomato. 50 The application of chitosan effectively controlled the C. fimbriata development in sweet potato, and chitosan treatment increased the superoxide dismutase and phenylalanine ammonia lyase activity but had no effect on malondialdehyde production. 38 Different from the previous studies, not only genes involved in the salicylic acid pathway but also the jasmonic acid pathway were upregulated upon chitosan treatment during C. heterostrophus infection.…”

Section: ■ Discussionmentioning

confidence: 97%

Molecular Mechanism Underlying Pathogenicity Inhibition by Chitosan in Cochliobolus heterostrophus

Yu,

Su,

Jia

et al. 2024

J. Agric. Food Chem.

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Chitosan, as a natural nontoxic biomaterial, has been demonstrated to inhibit fungal growth and enhance plant defense against pathogen infection. However, the antifungal pattern and mechanism of how chitosan application evokes plant defense are poorly elucidated. Herein, we provide evidence that chitosan exposure is fungicidal to C. heterostrophus. Chitosan application impairs conidia germination and appressorium formation of C. heterostrophus and has a pronounced effect on reactive oxygen species production, thereby preventing infection in maize. In addition, the toxicity of chitosan to C. heterostrophus requires Mkk1 and Mps1, two key components in the cell wall integrity pathway. The Δmkk1 and Δmps1 mutants were more tolerant to chitosan than the wild-type. To dissect chitosan-mediated plant defense response to C. heterostrophus, we conducted a metabolomic analysis, and several antifungal compounds were upregulated in maize upon chitosan treatment. Taken together, our findings provide a comprehensive understanding of the mechanism of chitosan-alleviated infection of C. heterostrophus, which would promote the application of chitosan in plant protection in agriculture. KEYWORDS: chitosan, Cochliobolus heterostrophus, cell wall integrity (CWI) pathway, reactive oxygen species (ROS), metabolomic ■ MATERIALS AND METHODS Materials. The wild-type strain of C. heterostrophus C4 (Tox1 + , MAT1−2, ATCC 48331) was stored in the laboratory and used for gene deletion. Complete medium with xylose (CMX) was used for C. heterostrophus cultivation. Fusarium graminearum, Fusarium verticillioide, and Setosphaeria turcica were grown on PDA. All strains were

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Section: ■ Discussionmentioning

confidence: 97%

Molecular Mechanism Underlying Pathogenicity Inhibition by Chitosan in Cochliobolus heterostrophus

Yu,

Su,

Jia

et al. 2024

J. Agric. Food Chem.

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“…It was found that 7-methoxycoumarin significantly inhibits the expression of hrpG and popA [ 22 ]. Hydroxycoumarins reduce the pathogenicity of bacterial wilt by inhibiting T3SS and the formation of biofilms [ 53 ]. In this study, active substances led to a significant decrease in the expression levels of hrpB , hrpX , and hrpF genes in the hrp gene cluster, as well as the expression of other virulence-related genes such as ripAW , ripAE , Rs5-4819 , Rs5-4374 , ace , egl3 , and pehB ( Figure 4 and Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

“…In this research, we employed the Rp strain MRS-5 (GDMCC1.3250), initially isolated in our lab from diseased mulberry plants located in Luogang, Guangdong Province. The cultivation process involved growing MRS-5 on a casamino acid–peptone–glucose (CPG) agar plate [ 53 ], with incubation at 30 °C for 72 h [ 48 ]. Subsequently, a single colony was selected and inoculated into 150 mL of CPG liquid medium.…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial Activity and Mechanism of Three Root Exudates from Mulberry Seedlings against Ralstonia pseudosolanacearum

Li,

Wang,

Liu

et al. 2024

Plants

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Bacterial wilt is a significant soil-borne disease that poses a threat to mulberry production yield and quality of agricultural production worldwide. However, the disease resistance mechanisms dependent on root exudates are not well understood. In this present study, we investigated the antibacterial mechanisms of the main active substances (erucamide, oleamide, and camphor bromide) present in mulberry root exudates (MRE) against Ralstonia pseudosolanacearum (Rp), the causal agent of bacterial wilt. Our findings revealed that these three active substances inhibited the growth activity of Rp by affecting the cell morphology and extracellular polysaccharide content, as well as triggering a burst of reactive oxygen species. The active substances induced oxidative stress, leading to a decrease in Rp growth. Additionally, the expression levels of key genes in the hrp gene cluster (hrpB, hrpX, and hrpF) and other virulence-related genes (such as ripAW, ripAE, Rs5-4819, Rs5-4374, ace, egl3, and pehB) were significantly reduced upon treatment with the active substances. Further pathogenicity experiments demonstrated that root exudates (at a concentration of 1.5 mg·mL−1) delayed or slowed down the occurrence of bacterial wilt in mulberry. These findings provide valuable insight into the antimicrobial mechanisms of MRE against Rp and lay a theoretical foundation for the development and application of biocontrol agents to control mulberry bacterial wilt.

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“…Addressing the challenges posed by Cd toxicity, which severely suppresses tobacco development and ion balance, foliar spraying with Fe 3 O 4 and ZnO CNS has been found to alleviate the adverse effects of Cd toxicity on tobacco growth [ 16 ]. Water-soluble daphnetin nanoparticles, specifically those grafted with carboxymethyl chitosan, have demonstrated effective suppression of tobacco bacterial wilt in pot experiments [ 38 ]. In addition, Cu composite rod-like nanoparticles have exhibited the ability to protect tobacco by inducing disease resistance [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated analyses of ionomics, phytohormone profiles, transcriptomics, and metabolomics reveal a pivotal role of carbon-nano sol in promoting the growth of tobacco plants

Wang,

Hua,

Liang

et al. 2024

BMC Plant Biol

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Background Carbon nano sol (CNS) can markedly affect the plant growth and development. However, few systematic analyses have been conducted on the underlying regulatory mechanisms in plants, including tobacco (Nicotiana tabacum L.). Results Integrated analyses of phenome, ionome, transcriptome, and metabolome were performed in this study to elucidate the physiological and molecular mechanisms underlying the CNS-promoting growth of tobacco plants. We found that 0.3% CNS, facilitating the shoot and root growth of tobacco plants, significantly increased shoot potassium concentrations. Antioxidant, metabolite, and phytohormone profiles showed that 0.3% CNS obviously reduced reactive oxygen species production and increased antioxidant enzyme activity and auxin accumulation. Comparative transcriptomics revealed that the GO and KEGG terms involving responses to oxidative stress, DNA binding, and photosynthesis were highly enriched in response to exogenous CNS application. Differential expression profiling showed that NtNPF7.3/NtNRT1.5, potentially involved in potassium/auxin transport, was significantly upregulated under the 0.3% CNS treatment. High-resolution metabolic fingerprints showed that 141 and 163 metabolites, some of which were proposed as growth regulators, were differentially accumulated in the roots and shoots under the 0.3% CNS treatment, respectively. Conclusions Taken together, this study revealed the physiological and molecular mechanism underlying CNS-mediated growth promotion in tobacco plants, and these findings provide potential support for improving plant growth through the use of CNS.

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Molecular mechanism of plant elicitor daphnetin-carboxymethyl chitosan nanoparticles against Ralstonia solanacearum by activating plant system resistance

Cited by 13 publications

References 54 publications

Molecular Mechanism Underlying Pathogenicity Inhibition by Chitosan in Cochliobolus heterostrophus

Molecular Mechanism Underlying Pathogenicity Inhibition by Chitosan in Cochliobolus heterostrophus

Antibacterial Activity and Mechanism of Three Root Exudates from Mulberry Seedlings against Ralstonia pseudosolanacearum

Integrated analyses of ionomics, phytohormone profiles, transcriptomics, and metabolomics reveal a pivotal role of carbon-nano sol in promoting the growth of tobacco plants

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