2024
DOI: 10.1101/2024.05.10.593530
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Molecular mechanism of plasmid elimination by the DdmDE defense system

L. Loeff,
D.W. Adams,
C. Chanez
et al.

Abstract: Seventh pandemic Vibrio cholerae strains contain two hallmark pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryo-EM to reveal the mechanistic basis for plasmid defense by DdmDE. A cryo-EM structure of the DdmD helicase-nuclease reveals that it adopts an auto-inhibited dimeric architecture. The prokaryotic Argonaute protein DdmE uses a DNA guide to target plasmid DNA. A structure of the DdmDE complex, validated by in vivo mutational studies, shows that DNA binding by Dd… Show more

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