Molecular mechanism of Rubisco activase: Dynamic assembly and Rubisco remodeling

Waheeda, Kazi; Kitchel, Heidi; Wang, Quan; Chiu, Po-Lin

doi:10.3389/fmolb.2023.1125922

Cited by 14 publications

(5 citation statements)

References 131 publications

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“…In C3 plants, glucose is primarily biosynthesized through the Calvin cycle. A crucial step in this process is the formation of glycerate-3-phosphate (3PGA) through the action of 1,5-bisphosphate ribulose carboxylase using CO 2 [28]. Rubiscos, which are photosynthetic CO 2 -fixing enzymes, are activated by Rubisco activase (Rcas).…”

Section: Discussionmentioning

confidence: 99%

“…NO plays a role in both abscisic acid (ABA)-and Ca 2+ -mediated stomatal closure and is located downstream of ROS, an important participant in the stomatal closure process [24,25]. In A. thaliana, ROS are mainly produced by NADPH oxidase and peroxidase at the plasma membrane [26,27], while NO is mainly produced by nitrate reductase (NR) and NOA1 and induces stomatal closure [28].…”

Section: Introductionmentioning

confidence: 99%

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Physiological and Proteomic Responses of the Tetraploid Robinia pseudoacacia L. to High CO2 Levels

Li,

Zhang,

Lei

et al. 2024

IJMS

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The increase in atmospheric CO2 concentration is a significant factor in triggering global warming. CO2 is essential for plant photosynthesis, but excessive CO2 can negatively impact photosynthesis and its associated physiological and biochemical processes. The tetraploid Robinia pseudoacacia L., a superior and improved variety, exhibits high tolerance to abiotic stress. In this study, we investigated the physiological and proteomic response mechanisms of the tetraploid R. pseudoacacia under high CO2 treatment. The results of our physiological and biochemical analyses revealed that a 5% high concentration of CO2 hindered the growth and development of the tetraploid R. pseudoacacia and caused severe damage to the leaves. Additionally, it significantly reduced photosynthetic parameters such as Pn, Gs, Tr, and Ci, as well as respiration. The levels of chlorophyll (Chl a and b) and the fluorescent parameters of chlorophyll (Fm, Fv/Fm, qP, and ETR) also significantly decreased. Conversely, the levels of ROS (H2O2 and O2·−) were significantly increased, while the activities of antioxidant enzymes (SOD, CAT, GR, and APX) were significantly decreased. Furthermore, high CO2 induced stomatal closure by promoting the accumulation of ROS and NO in guard cells. Through a proteomic analysis, we identified a total of 1652 DAPs after high CO2 treatment. GO functional annotation revealed that these DAPs were mainly associated with redox activity, catalytic activity, and ion binding. KEGG analysis showed an enrichment of DAPs in metabolic pathways, secondary metabolite biosynthesis, amino acid biosynthesis, and photosynthetic pathways. Overall, our study provides valuable insights into the adaptation mechanisms of the tetraploid R. pseudoacacia to high CO2.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physiological and Proteomic Responses of the Tetraploid Robinia pseudoacacia L. to High CO2 Levels

Li,

Zhang,

Lei

et al. 2024

IJMS

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“…The stability in carboxylation efficiency observed in the GM plants under the three collection stages in both irrigation treatments could potentially be linked to enhanced protection against the degradation of Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). Another probable reason was that ribulose activase, the enzyme that regulates Rubisco activation [39], was the most expressed enzyme identified via RNA-seq, with the GM plants under water deficit having 9% more transcripts of this enzyme than the NGM plants under the same irrigation conditions and 18% more transcripts than the NGM plants in the control treatment. Furthermore, the elevated protection against the degradation of this enzyme after re-irrigation in the GM plants may have contributed to a better cellular redox balance, thereby creating a more favorable cellular environment for its preservation and activity.…”

Section: Discussionmentioning

confidence: 99%

Calcium-Dependent Protein Kinase 5 (OsCPK5) Overexpression in Upland Rice (Oryza sativa L.) under Water Deficit

da Cruz,

Rocha,

Lanna

et al. 2023

Plants

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Water deficit significantly affects global crop growth and productivity, particularly in water-limited environments, such as upland rice cultivation, reducing grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring homologous genes that are linked to enhanced drought tolerance through the use of genomic data from model organisms can aid in the functional validation of target species. We evaluated the upland rice OsCPK5 gene, an A. thaliana AtCPK6 homolog, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed using a semi-automated phenotyping platform under two irrigation conditions: regular watering, and water deficit applied 79 days after seeding, lasting 14 days, followed by irrigation at 80% field capacity. The physiological data and leaf samples were collected at reproductive stages R3, R6, and R8. The genetically modified (GM) plants consistently exhibited higher OsCPK5 gene expression levels across stages, peaking during grain filling, and displayed reduced stomatal conductance and photosynthetic rate and increased water-use efficiency compared to non-GM (NGM) plants under drought. The GM plants also exhibited a higher filled grain percentage under both irrigation conditions. Their drought susceptibility index was 0.9 times lower than that of NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. The NGM plants under water deficit exhibited more leaf senescence, while the OsCPK5-overexpressing plants retained their green leaves. Overall, OsCPK5 overexpression induced diverse drought tolerance mechanisms, indicating the potential for future development of more drought-tolerant rice cultivars.

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“…The stability in carboxylation efficiency observed in GM plants at the three collect stages, in both irrigation treatments, could potentially be linked to enhanced protection against the degradation of Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). Another probable reason was that ribulose activase, the enzyme that regulates Rubisco activation (Waheeda et al, 2023), was the most expressed enzyme identified by RNAseq, with GM plants under water deficit having 9% more transcripts of this enzyme than plants NGM in the same irrigation condition, and 18% more than the NGM plants in the control treatment. Furthermore, the elevated protection against the degradation of this enzyme after re-irrigation in GM plants might have contributed to a better cellular redox balance, thereby creating a a more favorable cellular environment for its preservation and activity.…”

Section: Discussionmentioning

confidence: 99%

Calcium-Dependent Protein Kinase 5 (<em>OSCPK5</em>) Overexpression in Upland Rice (<em>Oryza sativa</em> L.) under Water Deficit

Da Cruz,

Rocha,

Lanna

et al. 2023

Preprint

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Water deficit greatly affects global crop growth and productivity, particularly in water-limited environments like upland rice cultivation, leading to reduced grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring orthologous genes, linked to enhanced drought tolerance, can aid functional validation in target species using genomic data from model organisms. Here, we studied the OsCPK5 gene in upland rice, an AtCPK6 ortholog from A. thaliana, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed under two conditions: water deficit applied 79 days after seeding, lasting 14 days, followed by 7 days of irrigation at 80% field capacity. Physiological data and samples were collected at R3, R6, and R8 stages. GM plants consistently exhibited higher OsCPK5 gene expression across stages, peaking during grain filling. During this phase, GM plants displayed reduced stomatal conductance, photosynthetic rate, and increased water use efficiency compared to non-GM plants under drought. GM plants also exhibited higher filled grain percentage in both irrigation conditions. Their drought susceptibility index was 0.9 times lower than NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. NGM plants under water deficit exhibited more leaf senescence, while OsCPK5-overexpressing plants retained green leaves. Overall, OsCPK5 overexpression induced diverse drought tolerance mechanisms, hinting at potential for future development of more drought-tolerant rice cultivars.

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Molecular mechanism of Rubisco activase: Dynamic assembly and Rubisco remodeling

Cited by 14 publications

References 131 publications

Physiological and Proteomic Responses of the Tetraploid Robinia pseudoacacia L. to High CO2 Levels

Physiological and Proteomic Responses of the Tetraploid Robinia pseudoacacia L. to High CO2 Levels

Calcium-Dependent Protein Kinase 5 (OsCPK5) Overexpression in Upland Rice (Oryza sativa L.) under Water Deficit

Calcium-Dependent Protein Kinase 5 (<em>OSCPK5</em>) Overexpression in Upland Rice (<em>Oryza sativa</em> L.) under Water Deficit

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