Molecular mechanism on cadmium-induced activity changes of catalase and superoxide dismutase

Wang, Jing; Zhang, Hao; Zhang, Tong; Zhang, Rui; Liu, Rutao; Chen, Yadong

doi:10.1016/j.ijbiomac.2015.02.037

Cited by 148 publications

(43 citation statements)

References 59 publications

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“…Atli et al (2006) reported that inhibition of CAT activity is related to direct binding of metal ions to-SH groups on the enzyme molecule. Wang et al (2015) demonstrated Cd-induced misfolding of CAT and SOD contributes to the reduced activity. Moreover, it is known that superoxide radicals can directly inhibit CAT 13 activity (Kamunde et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

et al. 2016

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“…The CAT and SOD activity changes appeared to relate to the direct binding of cadmium via electrostatic forces, leading to enzyme misfolding in vitro (Figure 7, KER14), and the subsequent induction of oxidative stress in vivo [47]. However, this was not investigated in other studies, which leads to low confidence for this mechanism.…”

Section: Discussion and Hypothesized Aop Frameworkmentioning

confidence: 99%

Programmed Effects in Neurobehavior and Antioxidative Physiology in Zebrafish Embryonically Exposed to Cadmium: Observations and Hypothesized Adverse Outcome Pathway Framework

Ruiter

Sippel

Bouwmeester

et al. 2016

IJMS

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Non-communicable diseases (NCDs) are a major cause of premature mortality. Recent studies show that predispositions for NCDs may arise from early-life exposure to low concentrations of environmental contaminants. This developmental origins of health and disease (DOHaD) paradigm suggests that programming of an embryo can be disrupted, changing the homeostatic set point of biological functions. Epigenetic alterations are a possible underlying mechanism. Here, we investigated the DOHaD paradigm by exposing zebrafish to subtoxic concentrations of the ubiquitous contaminant cadmium during embryogenesis, followed by growth under normal conditions. Prolonged behavioral responses to physical stress and altered antioxidative physiology were observed approximately ten weeks after termination of embryonal exposure, at concentrations that were 50–3200-fold below the direct embryotoxic concentration, and interpreted as altered developmental programming. Literature was explored for possible mechanistic pathways that link embryonic subtoxic cadmium to the observed apical phenotypes, more specifically, the probability of molecular mechanisms induced by cadmium exposure leading to altered DNA methylation and subsequently to the observed apical phenotypes. This was done using the adverse outcome pathway model framework, and assessing key event relationship plausibility by tailored Bradford-Hill analysis. Thus, cadmium interaction with thiols appeared to be the major contributor to late-life effects. Cadmium-thiol interactions may lead to depletion of the methyl donor S-adenosyl-methionine, resulting in methylome alterations, and may, additionally, result in oxidative stress, which may lead to DNA oxidation, and subsequently altered DNA methyltransferase activity. In this way, DNA methylation may be affected at a critical developmental stage, causing the observed apical phenotypes.

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“…Growing evidence indicates that the accumulation of Cd has a significant negative impact on organisms via the induction of oxidative stress mediated by the excessive generation of ROS (Wang et al 2015). CAT is one of the essential enzymes to regulate the level of ROS in organisms.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning, Characterization, and Expression of a Catalase Gene in the Japanese Scallop Mizuhopecten yessoensis Induced in the Presence of Cadmium

2016

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 Cadmium (Cd) is known to influence the oxidative status of marine organisms and can induce the formation of reactive oxygen species (ROS). Catalase (CAT) is one of the important enzymes involved in scavenging high levels of ROS. In present study, we cloned CAT cDNA and investigated the response of this enzyme at the transcriptional level in the Japanese scallop Mizuhopecten yessoensis exposed to Cd. The full-length CAT cDNA (MyCAT) of 1,870 nucleotides including a 57 bp 5'-UTR, a coding sequence of 1,500 bp and a 313 bp 3'-UTR were identified from the scallop. The deduced amino acid sequence of MyCAT corresponds to 499 amino acids with predicted molecular weight of 56.48 kDa and contains highly conserved motifs of the proximal heme-binding site RLFSYSTH, proximal active signature FNRERIPERVVHAKGGG and three catalytic amino acid residues His 72 , Asn 145 , and Tyr 355 . Its significant homology to CATs from multiple alignments revealed that MyCAT had a high identity with CATs from other mollusks. CAT mRNA expression analysis revealed that expression level was highest in the digestive gland ( p < 0.01) but weak in muscle. Following exposure to 200 and 400 µg/l of Cd, a high amount of Cd was found to have accumulated in the digestive gland and CAT mRNA expression had significantly increased in this organ among 7-day exposed scallops (p < 0.001). The result demonstrated that antioxidant enzymes such as CAT play important roles in counteracting Cd stress in M. yessoensis.

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Molecular mechanism on cadmium-induced activity changes of catalase and superoxide dismutase

Cited by 148 publications

References 59 publications

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

Chronic waterborne zinc and cadmium exposures induced different responses towards oxidative stress in the liver of zebrafish

Programmed Effects in Neurobehavior and Antioxidative Physiology in Zebrafish Embryonically Exposed to Cadmium: Observations and Hypothesized Adverse Outcome Pathway Framework

Molecular Cloning, Characterization, and Expression of a Catalase Gene in the Japanese Scallop Mizuhopecten yessoensis Induced in the Presence of Cadmium

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