Molecular mechanism triggered by co-metabolic biodegradation of azo dyestuff by Klebsiella sp. KL-1: Based on enzymatic and proteomic responsiveness

Zhang, Qingyun; Chen, Lijia; Xie, Xuehui; Qin, Yiting; Xu, Dayong; Zhang, Xiaoying; Liu, Xiaoyue; Liu, Na

doi:10.1016/j.jwpe.2024.105339

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2024

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Cited by 2 publications

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A review on recent insights of azoreductases mediated dye degradation: A sustainable approach for bioremediation of industrial wastewater

Veluchamy,

Jeyabalan,

Singh

et al. 2024

Journal of Water Process Engineering

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A review on recent insights of azoreductases mediated dye degradation: A sustainable approach for bioremediation of industrial wastewater

Veluchamy,

Jeyabalan,

Singh

et al. 2024

Journal of Water Process Engineering

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Grasping the constraints of pure bacterial strains for the complete catabolism of micropollutants: a proteomic and kinetic study

Lopez Gordillo,

Trueba-Santiso,

Smith

et al. 2024

Preprint

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Research into the microbial degradation of organic micropollutants (OMP) often involves monitoring depletion of the parent compound and analyzing the biotransformation pathways that can lead to the production of metabolites, some being toxic, and/or to their mineralization. For the antibiotic sulfamethoxazole (SMX), previous studies testing a range of SMX concentration (mg down to ng L-1), have shown incomplete biotransformation of the parent SMX. This occurred both during wastewater treatment with mixed microbial communities and in studies with pure bacterial strains acclimated to SMX. This study explores the mechanism of SMX biotransformation and relationships with the proteome profile as possible reasons for the incomplete degradation of the parent SMX. Microbacterium sp BR1 served as an acclimated bacterial degrader of SMX in the range of micrograms L-1 to ng L-1. Depletion of the SMX was incomplete whereas the metabolite 3-amino-5-methylisoxazole (3A5MI) accumulated. The activity of the enzymes for the initial transformation of the parent SMX (SadA) was higher than that of further biotransformation steps (SadB). These results showcase that even a highly sensitive and metabolically active strain at very low SMX concentrations may require complementary enzymatic machineries to degrade metabolites that have an inhibitory impact in the biodegradation and persistence of this antibiotic.

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Molecular mechanism triggered by co-metabolic biodegradation of azo dyestuff by Klebsiella sp. KL-1: Based on enzymatic and proteomic responsiveness

Cited by 2 publications

References 51 publications

A review on recent insights of azoreductases mediated dye degradation: A sustainable approach for bioremediation of industrial wastewater

A review on recent insights of azoreductases mediated dye degradation: A sustainable approach for bioremediation of industrial wastewater

Grasping the constraints of pure bacterial strains for the complete catabolism of micropollutants: a proteomic and kinetic study

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