Molecular mechanisms behind the cold and hot adaptation in extremozymes

Angelin, J.; Kavitha, M.

doi:10.1016/b978-0-323-90274-8.00013-7

Cited by 8 publications

(2 citation statements)

References 139 publications

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“…Colonies A2 and A4 displayed the best hydrolytic potential on solid starch-agar medium, giving zones of starch digestion of 21 + 0.1 mm and 25 + 0.1 mm, respectively (Table 1). It is presupposed that the A2 isolate is a mesophile while A4 is a thermophile because the optimum temperature of growth and amylase activity for the A2 isolate was at 37 0 C; on the other hand, the clear zone of hydrolysis measured by diameter (mm) increased at 45 0 C incubation temperature for the A4 isolate and according to Angelin and Kavitha (2022), hydrolytic bacteria thriving at temperatures between 40 0 C to 85 0 C are thermophilic and often good producers of thermostable amylases.…”

Section: Results and Discussion Bacterial Isolates From Soil And Bake...mentioning

confidence: 99%

AMYLOLYTIC POTENTIAL OF Arthrobacter sp. AND Bacillus aerius FROM WASTE DUMP-SITES OF TWO BAKERIES LOCATED WITHIN KADUNA METROPOLIS, NIGERIA

Obafunmi,

Bakori,

Lawal

2023

FJS

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Bakery waste dumpsites are believed to harbour a variety of bacteria due to the presence of organic materials like flour, sugar, and other food residues. The conditions in these environments support the growth of bacteria with the ability to degrade starch into simple sugars because they produce amylases, an enzyme pivotal to the food and beverage production. As Amylases progressively gain relevance in several other starch-based processes, their availability becomes insufficient, and the need for amylases with unique and superior efficiency increases. The focus of this study was to isolate and characterize bacteria with amylase activity from the soil of bakery waste dumpsites to provide valuable insight into cheap sources of amylolytic bacteria and their potential biotechnological applications. The starch-agar medium was used to isolate and screen amylase-producing bacteria by streak plate method. Two isolates, A2 and A4, showing the most hydrolytic activities of 21 + 0.1 mm and 25 + 0.1 mm, respectively, were identified by their cultural and cellular morphologies, biochemical characteristics, 16S rRNA gene sequence, and nearest phylogenetic neighbour as Arthrobacter sp. and Bacillus aerius. Based on their culture and amylolytic temperatures, Arthrobacter sp. (37 0C) and Bacillus aerius (45 0C) tend to produce amylases that can be explored in low to moderate and moderate to torrid industrial processes, respectively.

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Section: Results and Discussion Bacterial Isolates From Soil And Bake...mentioning

confidence: 99%

AMYLOLYTIC POTENTIAL OF Arthrobacter sp. AND Bacillus aerius FROM WASTE DUMP-SITES OF TWO BAKERIES LOCATED WITHIN KADUNA METROPOLIS, NIGERIA

Obafunmi,

Bakori,

Lawal

2023

FJS

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“…We could not answer how purification relates to the cold activity of lipase and esterase. Previous studies have shown that cold-adaptation processes of cold-active enzymes, such as lipases and esterases, do not indicate any directional trend; a wide range of solutions evolved, during enzyme evolution, some of which had counterproductive consequences such as activity-stability trade-offs [ 147 ] characterised by increase cold activity with consequent poor stability. The resolved crystal structures were reviewed in Table 3 .…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues

Matinja

Leow

Oslan

et al. 2022

IJMS

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Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase.

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Industrial Marvels of Extreme Microbial Adaptations

Priscilla,

Saleena

2024

Industrial Microbiology and Biotechnology

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Molecular mechanisms behind the cold and hot adaptation in extremozymes

Cited by 8 publications

References 139 publications

AMYLOLYTIC POTENTIAL OF Arthrobacter sp. AND Bacillus aerius FROM WASTE DUMP-SITES OF TWO BAKERIES LOCATED WITHIN KADUNA METROPOLIS, NIGERIA

AMYLOLYTIC POTENTIAL OF Arthrobacter sp. AND Bacillus aerius FROM WASTE DUMP-SITES OF TWO BAKERIES LOCATED WITHIN KADUNA METROPOLIS, NIGERIA

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues

Industrial Marvels of Extreme Microbial Adaptations

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