Molecular mechanisms by which hormones and cytokines regulate cell junction dynamics in the testis

Lui, Wing Yiu; Lee, Will M.

doi:10.1677/jme-08-0174

Cited by 21 publications

(23 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was further emphasized by the results of our western blot analysis, which clearly indicated the presence of 12 kDa INSL3 in both interstitial and seminiferous compartments. These findings suggest that INSL3 enters the seminiferous compartment across the blood-testis barrier (BTB), which is created by inter-Sertoli tight junctions and adherens junctions (Lui & Lee 2009 be attributable to the opening of the BTB and take place concomitantly when physiological disassembly of tight junctions occurs to allow the passage of preleptotene spermatocytes across the BTB. This is based on the findings that the BTB separates mitotic spermatogonia from meiotic germ cells and is periodically disassembled to allow the passage of preleptotene spermatocytes across the barrier (Lui & Lee 2009, Mruk & Cheng 2010, Cheng & Mruk 2012.…”

Section: Discussionmentioning

confidence: 99%

“…These findings suggest that INSL3 enters the seminiferous compartment across the blood-testis barrier (BTB), which is created by inter-Sertoli tight junctions and adherens junctions (Lui & Lee 2009 be attributable to the opening of the BTB and take place concomitantly when physiological disassembly of tight junctions occurs to allow the passage of preleptotene spermatocytes across the BTB. This is based on the findings that the BTB separates mitotic spermatogonia from meiotic germ cells and is periodically disassembled to allow the passage of preleptotene spermatocytes across the barrier (Lui & Lee 2009, Mruk & Cheng 2010, Cheng & Mruk 2012. Although no studies to date have identified the possible sites of action of INSL3 in the boar testis, RXFP2 mRNA and/or protein have been shown to be expressed in Leydig cells, germ cells, and/or Sertoli cells in different species, including humans (Anand- Ivell et al 2006a), rats (Kawamura et al 2004, Anand-Ivell et al 2006a), mice (Anand-Ivell et al 2006a, Feng et al 2007, roe deer (Hombach-Klonisch et al 2004), and goats .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Minagawa¹,

Sagata²,

Pitia³

et al. 2014

Journal of Endocrinology

View full text Add to dashboard Cite

Relaxin-like factor (RLF), now mainly known as insulin-like factor 3 (INSL3), is essential for testis descent during fetal development; however, its function in the adult testis is still being elucidated. As a major step toward understanding the as-yet-unknown function of INSL3 in boars, this study aimed to develop a time-resolved fluoroimmunoassay for boar INSL3, characterize the dynamics of INSL3 expression during development, and demonstrate the expression of the INSL3 hormone-receptor system in the testis. All samples were collected from Duroc boars. The sensitivity of the assay system established was 8.2 pg/well (164 pg/ml), and no cross-reactivity with other hormones, such as porcine relaxin, was observed. Circulating INSL3 was shown to increase progressively during development. INSL3 secreted from the Leydig cells was released not only into the blood circulation but also into the interstitial and seminiferous compartments in sufficient concentrations. A testicular fractionation study revealed that its receptor RXFP2 transcripts were expressed mainly in testicular germ cells. In addition, INSL3 bound to the germ cell membranes in a hormone-specific and saturable manner. These results reveal that INSL3 secreted into the interstitial compartment from the Leydig cells is transported into the seminiferous compartments, where its receptor RXFP2 is expressed mainly in the germ cells to which INSL3 binds, suggesting that INSL3 functions as a paracrine factor on seminiferous germ cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Minagawa¹,

Sagata²,

Pitia³

et al. 2014

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Cytokine-mediated down-regulation of junction proteins results in an increase in BTB permeability [12]. Several studies have shown that transforming growth factor-b 3 (TGFB) and tumor necrosis factor a (TNF) are involved in regulating the transcription of claudin11, occludin, and zonula occluden 1 (ZO1), which are major components of tight junctions [13,14].…”

Section: Introductionmentioning

confidence: 99%

Mono-(2-Ethylhexyl) Phthalate-Induced Disruption of Junctional Complexes in the Seminiferous Epithelium of the Rodent Testis Is Mediated by MMP21

Yao

Lin

Richburg

2010

Biology of Reproduction

View full text Add to dashboard Cite

Tight junctions between Sertoli cells of the testicular seminiferous epithelium establishes the blood-testis barrier (BTB) and creates a specialized adluminal microenvironment above the BTB that is required for the development of the germ cells that reside there. Actin filament-based anchoring junctions between Sertoli cells and germ cells are important for maintaining close physical contact between these cells as well as regulating the release of mature spermatids into the lumen. Previously, we reported that Sertoli cell injury in rodents after mono-(2-ethylhexyl) phthalate (MEHP) exposure results in the activation of matrix metalloproteinase 2 (MMP2) and increases the sensitivity of germ cells to undergo apoptosis. A disruption in the physical association between Sertoli cells and germ cells and premature loss of germ cells from the seminiferous epithelium has been widely described after phthalate treatment. In this study, we investigate the functional participation of MMP2 in the mechanism underlying MEHP-induced disruption of junction complexes and the resultant loss of germ cells. Exposure of C57BL/6J mice to MEHP (1 g/kg, oral gavage) decreased the expression of occludin in the tight junctions between Sertoli cells and caused gaps between adjacent Sertoli cells as observed by transmission electron microscopy. A reduced expression of laminin-gamma3 and beta1-integrin in apical ectoplasmic specializations between Sertoli cells and germ cells in a time-dependent manner was also observed. Treatment with specific MMP2 inhibitors (TIMP2 and SB-3CT) both in vitro and in vivo significantly suppressed MEHP-induced germ cell sloughing and changes in the expression of these junctional proteins, indicating that MMP-2 plays a primary role in this process. Furthermore, the detachment of germ cells from Sertoli cells appears to be independent of the apoptotic signaling process since MEHP-induced germ cell detachment from Sertoli cells could not be prevented by the addition of a pan-caspase inhibitor (z-VAD-FMK).

show abstract

“…tiation until maturation through the spermatogenesis (Lui and Lee, 2009). To observe the effect of WSF on Leydig cells, testosterone in serum was measured by ELISA.…”

Section: Resultsmentioning

confidence: 99%

Heavy oil fraction induces the dysplastic sperm in male mouse

et al. 2011

View full text Add to dashboard Cite

-Heavy oil is one of the most serious pollutants in marine ecosystem. The poisonous influences of the chemical substances contained in heavy oil on many kinds of marine organisms are widely studied. However, the influence of the chemical compounds in heavy oil on our health has not been cleared yet. In order to reveal the poisonous influences of these chemical compounds on mammalian reproductive system, water-soluble fraction (WSF) extracted from heavy oil was administrated to mice for 2 weeks. WSF-administrated mice were crossed with either WSF-or distilled water-administrated group for mating experiment. When WSF-administrated male mice were used as a father, it reduced not only mating ratio, but also neonatal male ratio. The numbers of sperms of WSF-administrated male mice were decreased. In addition, abnormality of sperms such as bent or twisted tail was increased approximately 6-fold by WSF intake. The level of testosterone in serum from WSF-administrated mice was lower than that from control mice. Testosterone is the most important for the spermatogenesis in vertebrate. It is supposed from these findings, the decrease in the number of sperms may relate with the reduction of sex hormone level in serum. It is suggested from these results that the chemical substances in WSF affected the sperm function in reproductive system of male mice.

show abstract

Molecular mechanisms by which hormones and cytokines regulate cell junction dynamics in the testis

Cited by 21 publications

References 70 publications

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Mono-(2-Ethylhexyl) Phthalate-Induced Disruption of Junctional Complexes in the Seminiferous Epithelium of the Rodent Testis Is Mediated by MMP21

Heavy oil fraction induces the dysplastic sperm in male mouse

Contact Info

Product

Resources

About