Molecular mechanisms for strengthening E-cadherin adhesion using a monoclonal antibody

Xie, Bin; Priest, Andrew Vae; Maker, Allison; Gumbiner, Barry M.; Sivasankar, Sanjeevi

doi:10.1016/j.bpj.2021.11.2769

Cited by 1 publication

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“…Modifications of the actin cortex, which surrounds the periphery of the cell underneath the plasma membrane, facilitates cancer cell movement as it provides traction forces to the cell [ 6 ]. Proteins involved in cancer cell adhesion have been targeted by different therapeutic approaches, including the use of miRNAs [ 7 , 8 ] and blocking monoclonal antibodies [ 9 , 10 ], in an attempt to avoid cancer spreading.…”

Section: Introductionmentioning

confidence: 99%

Plasmonic Biosensing for Label-Free Detection of Two Hallmarks of Cancer Cells: Cell-Matrix Interaction and Cell Division

et al. 2022

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Two key features of cancer cells are sustained proliferation and invasion, which is preceded by a modification of the adhesion properties to the extracellular matrix. Currently, fluorescence-based techniques are mainly used to detect these processes, including flow cytometry and fluorescence resonance energy transfer (FRET) microscopy. We have previously described a simple, fast and label-free method based on a gold nanohole array biosensor to detect the spectral response of single cells, which is highly dependent on the actin cortex. Here we used this biosensor to study two cellular processes where configuration of the actin cortex plays an essential role: cell cycle and cell–matrix adhesion. Colorectal cancer cells were maintained in culture under different conditions to obtain cells stopped either in G0/G1 (resting cells/cells at the initial steps of cell growth) or G2 (cells undergoing division) phases of the cell cycle. Data from the nanohole array biosensor showed an ability to discriminate between both cell populations. Additionally, cancer cells were monitored with the biosensor during the first 60 min after cells were deposited onto a biosensor coated with fibronectin, an extracellular matrix protein. Spectral changes were detected in the first 20 min and increased over time as the cell–biosensor contact surface increased. Our data show that the nanohole array biosensor provides a label-free and real-time procedure to detect cells undergoing division or changes in cell–matrix interaction in both clinical and research settings.

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