Molecular Mechanisms in Yeast Carbon Metabolism: Lipid Metabolism and Lipidomics

Ploier, Birgit; Daum, Günther; Petrovič, Uroš

doi:10.1007/978-3-662-45782-5_8

Cited by 7 publications

(3 citation statements)

References 260 publications

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“…The burying beetle could benefit from these pathways because insects cannot synthesize the sterol precursors needed to form lipid biostructures, steroid hormones and developmental regulators24344. Yeasts often secrete sterols45 and if Yarrowia secrete essential sterol precursors in the rectum or on the carcass, these molecules could be utilized by the host, as previously suggested for fungal symbionts in other beetles and in leafcutter ants464748. The role of these potential symbionts in sterol production in the rectum is supported by the finding that both sterol modifying enzymes (for example, sterol reductase) and sterol transport proteins (lipophorins) are expressed at high levels specifically in the host rectum (Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

The digestive and defensive basis of carcass utilization by the burying beetle and its microbiota

et al. 2017

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Insects that use ephemeral resources must rapidly digest nutrients and simultaneously protect them from competitors. Here we use burying beetles (Nicrophorus vespilloides), which feed their offspring on vertebrate carrion, to investigate the digestive and defensive basis of carrion utilization. We characterize gene expression and microbiota composition in the gut, anal secretions, and on carcasses used by the beetles. We find a strict functional compartmentalization of the gut involving differential expression of immune effectors (antimicrobial peptides and lysozymes), as well as digestive and detoxifying enzymes. A distinct microbial community composed of Firmicutes, Proteobacteria and a clade of ascomycetous yeasts (genus Yarrowia) is present in larval and adult guts, and is transmitted to the carcass via anal secretions, where the yeasts express extracellular digestive enzymes and produce antimicrobial compounds. Our results provide evidence of potential metabolic cooperation between the host and its microbiota for digestion, detoxification and defence that extends from the beetle's gut to its nutritional resource.

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Section: Discussionmentioning

confidence: 99%

The digestive and defensive basis of carcass utilization by the burying beetle and its microbiota

et al. 2017

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“… Intracellular biosynthesis pathways for ergosterol, sphingolipids, and phospholipids in S. cerevisiae based on references 84 to 86 . In the case of blocked sphingolipid or phospholipid biosynthesis, long-chain (unsaturated) fatty acids [LC(U)FA] are imported to the cell (dashed arrows).…”

Section: Resultsmentioning

confidence: 99%

Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids

et al. 2023

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“…This step does not require any oxygen. Cells are also able to downregulate the sterol biosynthesis or acetylate the sterols in order to secrete excess amounts of them into the media (Ploier, Daum, & Petrovič, ).…”

Section: Introductionmentioning

confidence: 99%

Sterol synthesis and cell size distribution under oscillatory growth conditions in Saccharomyces cerevisiae scale‐down cultivations

Marbà-Ardébol

Bockisch

Neubauer

et al. 2017

Yeast

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Physiological responses of yeast to oscillatory environments as they appear in the liquid phase in large-scale bioreactors have been the subject of past studies. So far, however, the impact on the sterol content and intracellular regulation remains to be investigated. Since oxygen is a cofactor in several reaction steps within sterol metabolism, changes in oxygen availability, as occurs in production-scale aerated bioreactors, might have an influence on the regulation and incorporation of free sterols into the cell lipid layer. Therefore, sterol and fatty acid synthesis in two- and three-compartment scale-down Saccharomyces cerevisiae cultivation were studied and compared with typical values obtained in homogeneous lab-scale cultivations. While cells were exposed to oscillating substrate and oxygen availability in the scale-down cultivations, growth was reduced and accumulation of carboxylic acids was increased. Sterol synthesis was elevated to ergosterol at the same time. The higher fluxes led to increased concentrations of esterified sterols. The cells thus seem to utilize the increased availability of precursors to fill their sterol reservoirs; however, this seems to be limited in the three-compartment reactor cultivation due to a prolonged exposure to oxygen limitation. Besides, a larger heterogeneity within the single-cell size distribution was observed under oscillatory growth conditions with three-dimensional holographic microscopy. Hence the impact of gradients is also observable at the morphological level. The consideration of such a single-cell-based analysis provides useful information about the homogeneity of responses among the population.

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Molecular Mechanisms in Yeast Carbon Metabolism: Lipid Metabolism and Lipidomics

Cited by 7 publications

References 260 publications

The digestive and defensive basis of carcass utilization by the burying beetle and its microbiota

The digestive and defensive basis of carcass utilization by the burying beetle and its microbiota

Cellular Responses and Targets in Food Spoilage Yeasts Exposed to Antifungal Prenylated Isoflavonoids

Sterol synthesis and cell size distribution under oscillatory growth conditions in Saccharomyces cerevisiae scale‐down cultivations

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