Molecular mechanisms involved in increased expression of a cytochrome P450 responsible for pyrethroid resistance in the housefly, <i>Musca domestica</i>

Tomita, Takashi; Liu, N; Smith, F. B.; Sridhar, P.; Scott, Jeffrey G.

doi:10.1111/j.1365-2583.1995.tb00018.x

Cited by 73 publications

(46 citation statements)

References 23 publications

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“…The insect P450 oxidases responsible for resistance have belonged to family 6, which, like the esterases, occur in Diptera as a cluster of genes (32). Members of the cluster may be expressed as multiple (up to five) alleles (33). Enhanced levels of oxidases in resistant insects result from constitutive overexpression rather than amplification (34,35).…”

Section: B Amentioning

confidence: 99%

Insecticide Resistance and Vector Control

Brogdon

McAllister²

1999

Journal of Agromedicine

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Section: B Amentioning

confidence: 99%

Insecticide Resistance and Vector Control

Brogdon

McAllister²

1999

Journal of Agromedicine

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“…While metabolic resistance has been generally attributed to the cytochrome P450s, esterases, and glutathione S-transferases, each of these are large gene families in the cattle tick with 115, 81, and 39 individual members, respectively (BELLGARD et al, 2012). As knowledge of genetics and genomics of arthropods and the cattle tick advances, it will become possible to specify a gene-mediated metabolic resistance mechanism as has been done for glutathione S-transferase-based DDT resistance in Anopheles gambiae (RANSON et al, 2001) and cytochrome P450-based pyrethroid resistance in Musca domestica (TOMITA et al, 1995). Biochemical synergist studies are often used to determine if metabolic resistance mechanisms are present in resistant populations of R.…”

Section: Pyrethroid Resistancementioning

confidence: 99%

Acaricide resistance mechanisms in Rhipicephalus (Boophilus) microplus

Guerrero

Lovis

Martins

2012

Rev. Bras. Parasitol. Vet.

194

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Acaricide resistance has become widespread in countries where cattle ticks, Rhipicephalus (Boophilus) microplus, are a problem. Resistance arises through genetic changes in a cattle tick population that causes modifications to the target site, increased metabolism or sequestration of the acaricide, or reduced ability of the acaricide to penetrate through the outer protective layers of the tick's body. We review the molecular and biochemical mechanisms of acaricide resistance that have been shown to be functional in R. (B.) microplus. From a mechanistic point of view, resistance to pyrethroids has been characterized to a greater degree than any other acaricide class. Although a great deal of research has gone into discovery of the mechanisms that cause organophosphate resistance, very little is defined at the molecular level and organophosphate resistance seems to be maintained through a complex and multifactorial process. The resistance mechanisms for other acaricides are less well understood. The target sites of fipronil and the macrocyclic lactones are known and resistance mechanism studies are in the early stages. The target site of amitraz has not been definitively identified and this is hampering mechanistic studies on this acaricide.Keywords: Cattle tick, resistance mechanisms, target site mutation, metabolism. ResumoA resistência aos acaricidas tornou-se amplamente difundida nos países onde os carrapatos bovinos, Rhipicephalus (Boophilus) microplus, são um problema. A resistência surge por meio de alterações genéticas em uma população de carrapatos que causam modificações no local de ação, aumento do metabolismo ou sequestro do acaricida, ou ainda redução na capacidade do acaricida em penetrar através das camadas protetoras do corpo do carrapato. Neste artigo, foram revisados os mecanismos moleculares e bioquímicos da resistência aos acaricidas que ocorrem em R. (B.) microplus. A partir de um ponto de vista dos mecanismos envolvidos, a resistência aos piretróides tem sido caracterizada em maior grau do que em qualquer outra classe de acaricida. Embora uma grande quantidade de pesquisas têm sido direcionada para a descoberta de mecanismos que causam resistência aos organofosforados, muito pouco é conhecido ao nível molecular, e essa resistência parece ser mantida por intermédio de um processo multifatorial e complexo. Os mecanismos de resistência para os demais acaricidas são bem menos compreendidos. Os alvos de ação do fipronil e das lactonas macrocíclicas são conhecidos, e os estudos dos mecanismos de ação envolvidos estão ainda em estágios iniciais. O alvo de ação do amitraz ainda não foi definitivamente identificado, e isso é limitante aos estudos dos mecanismos envolvidos na resistência a esse acaricida.Palavras-chave: Carrapato bovino, mecanismos de resistência, mutação no local de ação, metabolismo.

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“…In the house fly (Musca domestica), CYP6A1 metabolizes the insecticides aldrin and heptachlor (10), and CYP6D1 has been linked to deltamethrin metabolism (11). Heterologous expression of Drosophila melanogaster CYP6A2 in Saccharomyces cerevisiae bioactivates some genotoxins (e.g., aflatoxin B 1 ) (12).…”

mentioning

confidence: 99%

Molecular cloning of a family of xenobiotic-inducible drosophilid cytochrome P450s: Evidence for involvement in host-plant allelochemical resistance

Danielson

MacIntyre

Fogleman

1997

Proc. Natl. Acad. Sci. U.S.A.

114

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Cytochrome P450s constitute a superfamily of genes encoding mostly microsomal hemoproteins that play a dominant role in the metabolism of a wide variety of both endogenous and foreign compounds. In insects, xenobiotic metabolism (i.e., metabolism of insecticides and toxic natural plant compounds) is known to involve members of the CYP6 family of cytochrome P450s. Use of a 3 RACE (rapid amplification of cDNA ends) strategy with a degenerate primer based on the conserved cytochrome P450 heme-binding decapeptide loop resulted in the amplification of four cDNA sequences representing another family of cytochrome P450 genes (CYP28) from two species of isoquinoline alkaloidresistant Drosophila and the cosmopolitan species Drosophila hydei. The CYP28 family forms a monophyletic clade with strong regional homologies to the vertebrate CYP3 family and the insect CYP6 family (both of which are involved in xenobiotic metabolism) and to the insect CYP9 family (of unknown function). Induction of mRNA levels for three of the CYP28 cytochrome P450s by toxic host-plant allelochemicals (up to 11.5-fold) and phenobarbital (up to 49-fold) corroborates previous in vitro metabolism studies and suggests a potentially important role for the CYP28 family in determining patterns of insect-host-plant relationships through xenobiotic detoxification.

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Molecular mechanisms involved in increased expression of a cytochrome P450 responsible for pyrethroid resistance in the housefly, Musca domestica

Cited by 73 publications

References 23 publications

Insecticide Resistance and Vector Control

Insecticide Resistance and Vector Control

Acaricide resistance mechanisms in Rhipicephalus (Boophilus) microplus

Molecular cloning of a family of xenobiotic-inducible drosophilid cytochrome P450s: Evidence for involvement in host-plant allelochemical resistance

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