Molecular mechanisms involved in Sertoli cell adaptation to glucose deprivation

Riera, María Fernanda; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Meroni, Silvina Beatriz; Cigorraga, Selva Beatriz

doi:10.1152/ajpendo.00235.2009

Cited by 84 publications

(52 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context, the transport of glucose through the plasma membrane is the rate-limiting step in glucose metabolism and, consequently, in lactate production (Riera et al 2001(Riera et al , 2009. Glucose enters the cell by carrier proteins called glucose transporters (known as GLUTs or SLC2A).…”

Section: Cox and Pgs In Sertoli Cellsmentioning

confidence: 99%

Cyclooxygenase and prostaglandins in somatic cell populations of the testis

Frungieri¹,

Calandra²,

Mayerhofer³

et al. 2015

Reproduction

View full text Add to dashboard Cite

Prostaglandins (PGs) are synthesized through the action of the rate-limiting enzyme cyclooxygenase (COX) and further specific enzymes. The development of Cox-deficient mice in the 1990s gave insights into the reproductive roles of PGs. Female Cox-knockout mice were subfertile or infertile. Interestingly, fertility was not affected in male mice deficient in Cox, suggesting that PGs may not be critical for the functioning of the testis. However, this conclusion has recently been challenged by observations of important roles for PGs in both physiological and pathological processes in the testis. The two key somatic cell types in the testis, Leydig and Sertoli cells, express the inducible isoenzyme COX2 and produce PGs. Testicular COX2 expression in these somatic cells is regulated by hormonal input (FSH, prolactin (PRL), and testosterone) as well as by IL1b. PGs modulate steroidogenesis in Leydig cells and glucose uptake in Sertoli cells. Hence, the COX2/PG system in Leydig and Sertoli cells acts as a local modulator of testicular activity, and consequently may regulate spermatogenic efficiency. In addition to its expression in Leydig and Sertoli cells, COX2 has been detected in the seminiferous tubule wall, and in testicular macrophages and mast cells of infertile patients. These observations highlight the possible relevance of PGs in testicular inflammation associated with idiopathic infertility. Collectively, these data indicate that the COX2/PG system plays crucial roles not only in testicular physiology (i.e., development, steroidogenesis, and spermatogenesis), but more importantly in the pathogenesis or maintenance of infertility status in the male gonad. Further studies of these actions could lead to new therapeutic approaches to idiopathic male infertility.Free German abstract: A German translation of this abstract is freely available at

show abstract

Section: Cox and Pgs In Sertoli Cellsmentioning

confidence: 99%

Cyclooxygenase and prostaglandins in somatic cell populations of the testis

Frungieri¹,

Calandra²,

Mayerhofer³

et al. 2015

Reproduction

View full text Add to dashboard Cite

show abstract

“…is observed even in the complete absence of glucose in a culture medium (33). The less intense but significant expression of MCT1 in spermatocytes and spermatids suggests the sufficient uptake of lactate and other monocarboxylates by MCT1; this is supported by the colocalization of CD147, which is involved in the trafficking of MCT molecules to the cell membrane.…”

Section: Resultsmentioning

confidence: 96%

“…Fresh frozen sections, 14-μm-thick, were fixed with 4% formaldehyde in 0.1 M phosphate buffer for 15 min and then acetylated with 0.25% acetic anhydride in 0.1 M triethanolamine-HCl (pH 8.0) for 10 min. Hybridization was performed at 42°C for 10 h with a hybridization buffer containing 33 P-labeled oligonucleotide probes (10,000 cpm/μL). The sections were rinsed at room temperature for 30 min in 2 × SSC (1 × SSC: 150 mM sodium chloride, 15 mM sodium citrate) containing 0.1% N-lauroylsarcosine sodium, then rinsed twice at 55°C for 40 min in 0.1 × SSC containing 0.1% N-lauroylsarcosine sodium, dehydrated through a graded series of ethanol, and air-dried.…”

mentioning

confidence: 99%

<b>Immunohistochemical localization of GLUT3, MCT1, and MCT2 in the testes of mice and rats: the use of different energy sources in </b><b>spermatogenesis </b>

et al. 2015

View full text Add to dashboard Cite

Lactate represents a preferential energy substrate of germ cells rather than glucose. Testicular Sertoli cells are believed to produce lactate and pyruvate and to supply these to germ cells, particularly spermatocytes and spermatids. Monocarboxylate transporter (MCT), responsible for the transport of lactate and other monocarboxylates via the cell membrane, is abundant in the testes and sperm (MCT1, MCT2, and MCT4). For the uptake of glucose, germ cells within the seminiferous tubules and sperm have been known to intensely express GLUT3. The present study investigated expression profiles of MCTs and GLUTs and revealed their cellular and subcellular localization in the mouse and rat testis. An in situ hybridization analysis showed significant expressions of MCT1, MCT2, and GLUT3 mRNA in the testis. Immunohistochemically, spermatogonia, spermatocytes, and spermatids expressed MCT1 on their cell surfaces in a stage-dependent manner: in some seminiferous tubules, an intense expression of MCT1 was unique to the spermatogonia. MCT2 was restricted to the tails of elongated spermatids and sperm. An intense immunoreactivity for GLUT3 was shared by spermatocytes, spermatids, and sperm. Sertoli cells were devoid of any immunoreactivities for MCT1, MCT2, and GLUT3. The predominant energy source of germ cells may be lactate and other monocarboxylates-especially for spermatogonia, but glucose and other hexoses may be responsible for an energy supply to spermatocytes and spermatids.For their survival and activity, cells use different energy sources to produce ATP under various conditions. Although it is not easy to characterize the energy sources for each cell, the identification of substrate-specific transporters can yield important information as to what energy is utilized. The testis with its continuous spermatogenesis is attractive for considering the energy supply system since more mature germ cells-spermatocytes and spermatidsare separated from the blood plasma by the bloodtestis barrier, whereas spermatogonia are outside this barrier. Furthermore, Sertoli cells play supportive and regulatory roles in terms of the energy metabolism of germ cells. It is generally believed that postmeiotic germ cells use lactate produced by Sertoli cells (15,25) and that spermatogonia utilize glucose as the major energy substrates (28). This idea is consistent with the location of the diffusion barrier constructed between the spermatogonia and spermatocytes, though glucose may cross the barrier (37) and be taken up by the spermatogenic cells (29). The uptake of glucose is regulated by the facilitated-diffusion glucose transporter family, GLUTs, until the transport equilibrates blood glucose and intracellular glucose. On the basis of sequence simi-

show abstract

“…Testicular energy metabolism shows some form of specificity in which lactate is the main substrate for energy (ATP) production in germ cells (Oliveira et al, 2012). In vitro studies with cultured Sertoli cells have shown that removing either insulin (Oliveira et al, 2012) or glucose (Riera et al, 2009) from the culture medium results in adaptation of the Sertoli cells to glucose transport as seen in modulated gene expression of glucose transporters (GLUT1 and GLUT3). However, Oliveira et al, (2012) have reported that the insulindeprived Sertoli cells in their in vitro experiment (which closely mimics T1DM) have reduced glucose utilization even when the gene expression of the glucose transporters is increased.…”

Section: Testicular Effects Energy Metabolism In the Testismentioning

confidence: 99%

Diabetes mellitus-induced male reproductive impairment: The role of natural products: A review

2017

J App Pharm Sci

View full text Add to dashboard Cite

Diabetes mellitus (DM) is a metabolic disorder characterized by persistent hyperglycaemia, with multi-organ complications. DM is known to trigger oxidative stress in virtually all tissues of the body including male reproductive organs. Several experimental and clinical studies have reported varying degrees of male reproductive impairment in DM. Disruption in the hypothalamic-pituitary-gonadal axis, abnormal testicular energy metabolism, testicular and epididymal oxidative stress and oxidative stress-induced germ cell apoptosis have been reported in DM. The use of natural products in the treatment of various diseases is gaining more attention in experimental and clinical studies for the management and/or treatment of various diseases. In this review, the effects of DM on male reproductive system at pre-testicular, testicular and post-testicular levels are discussed. The role of various natural products including whole plant extracts and some pure compounds isolated from plants, with their suggested mechanism of actions is also reviewed.

show abstract

Molecular mechanisms involved in Sertoli cell adaptation to glucose deprivation

Cited by 84 publications

References 52 publications

Cyclooxygenase and prostaglandins in somatic cell populations of the testis

Cyclooxygenase and prostaglandins in somatic cell populations of the testis

<b>Immunohistochemical localization of GLUT3, MCT1, and MCT2 in the testes of mice and rats: the use of different energy sources in </b><b>spermatogenesis </b>

Diabetes mellitus-induced male reproductive impairment: The role of natural products: A review

Contact Info

Product

Resources

About