Molecular Mechanisms of Campylobacter Biofilm Formation and Quorum Sensing

Püning, Christoph; Su, Yulan; Lu, Xiaonan; Gölz, Greta

doi:10.1007/978-3-030-65481-8_11

Cited by 19 publications

(13 citation statements)

References 109 publications

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“…(55) have shown that iron acquisition genes are down-regulated during acid stress, so it can be hypothesised that exposure of C. jejuni to LEO also led to stress responses in these bacteria. It is important to note that the iron-uptake system is involved in the colonisation, biofilm formation and pathogenicity of this pathogen (56,57), so down-regulation of this pathway might lead to reduction of these processes. Gene cstA, which encodes a carbon starvation protein, was also down-regulated, and this gene is known to be involved in C. jejuni motility and autoagglutination (58).…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Ramić

Bučar

Kunej

et al. 2021

Appl Environ Microbiol

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New approaches for the control of Campylobacter jejuni biofilms in the food industry are being studied intensively. Natural products are promising alternative antimicrobial substances to control biofilm production, with particular emphasis on plant extracts. Dried flowers of Lavandula angustifolia were used to produce LEO, LEF, and LEW. The chemical compositions determined for these Lavandula preparations included seven major compounds that were selected for further testing. These were tested against C. jejuni , for biofilm degradation and removal. Next-generation sequencing was used to study the molecular mechanisms underlying LEO actions against C. jejuni adhesion and motility. Analysis of LEO revealed 1,8-cineol, linalool and linalyl acetate as the main components. For LEF and LEW, the main components were phenolic acid glycosides, with flavonoids rarely present. The minimal inhibitory concentrations of the Lavandula preparations and pure compounds against C. jejuni ranged from 0.2 mg/mL to 1 mg/mL. LEO showed the strongest biofilm degradation. The reduction of C. jejuni adhesion was by ≥1 log 10 CFU/mL, which satisfies European Food Safety Authority recommendations. Lavandula preparations reduced C. jejuni motility by almost 50%, which consequently can impact upon biofilm formation. These data are in line with the transcriptome analysis of C. jejuni , where LEO down-regulated genes important for biofilm formation. LEW also showed good antibacterial and anti-biofilm effects, particularly against adhesion and motility mechanisms. This defines an innovative approach using alternative strategies and novel targets to combat bacterial biofilm formation, and hence the potential to develop new effective agents with biofilm-degrading activities. Importance The Lavandula preparations used in this study are found to be effective against C. jejuni , a common foodborne pathogen. They show anti-biofilm properties at sub-inhibitory concentrations in terms of promoting biofilm degradation and inhibiting cell adhesion and motility, which are involved in the initial steps of biofilm formation. These results are confirmed by transcriptome analysis, which highlights the effect of Lavandula essential oil on C. jejuni biofilm properties. We show that the waste material from the hydrodistillation of Lavandula has particular anti-biofilm effects, suggesting that it may find reuse for industrial purposes. This study highlights the need for efforts directed towards such innovative approaches and alternative strategies against biofilm formation and maintenance by developing new naturally derived agents with anti-biofilm activities.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Ramić

Bučar

Kunej

et al. 2021

Appl Environ Microbiol

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“…Nevertheless, it is still unclear whether AI-2 in C. jejuni represents a true QS signaling molecule or whether it is a metabolic by-product of a crucial central metabolic methyl cycle pathway [ 45 ]. To date, AI-2 receptors have still not been identified [ 46 ], and mechanisms by which bioactive compounds affect C. jejuni signaling should be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Rhodiola rosea Reduces Intercellular Signaling in Campylobacter jejuni

2022

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Campylobacter jejuni is a major foodborne pathogen and the leading cause of bacterial gastroenteritis, i.e., campylobacteriosis. Besides searching for novel antimicrobials, identification of new targets for their action is becoming increasingly important. Rhodiola rosea has long been used in traditional medicine. Ethanolic extracts from the roots and rhizomes of the plant contain a wide range of bioactive compounds with various pharmacological activities. In this study, cultivated plant materials have been used, i.e., “Mattmark” and “Rosavine”. Through optimized protocols, we obtained fractions of the initial ethanolic extracts rich in most important bioactive compounds from R. rosea, including salidroside, rosavins, proanthocyanidins (PACs), and flavonoids. The antimicrobial activity in relation to the chemical composition of the extracts and their fractions was studied with an emphasis on C. jejuni AI-2-mediated intercellular signaling. At concentration 15.625 mg/L, bioluminescence reduction rates varied from 27% to 72%, and the membrane remained intact. Fractions rich in PACs had the strongest antimicrobial effect against C. jejuni, with the lowest minimal inhibitory concentrations (MICs) (M F3 40%: 62.5 mg/L; R F3 40%: 250 mg/L) and the highest intercellular signaling reduction rates (M F3 40%: 72%; R F3 40%: 65%). On the other hand, fractions without PACs were less effective (MICs: M F5 PVP: 250 mg/L; R F5 PVP: 1000 mg/L and bioluminescence reduction rates: M F5 PVP: 27%; R F5 PVP: 43%). Additionally, fractions rich in flavonoids had strong antimicrobial activity (MICs: M F4 70%: 125 mg/L; R F4 70%: 250 mg/L and bioluminescence reduction rates: M F4 70%: 68%; R F4 70%: 50%). We conclude that PACs and flavonoids are crucial compound groups responsible for the antimicrobial activity of R. rosea roots and rhizomes in C. jejuni.

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“…Properties such as intercellular signaling with AI-2 signaling molecule, motility, and chemotaxis allow them to form biofilms or colonize existing biofilms on abiotic surfaces such as polystyrene, glass, and stainless steel. Within biofilms, Campylobacters are protected from antimicrobial agents that penetrate the biofilm matrix slowly and poorly [ 7 ]. Various approaches are used to control Campylobacters in the food production chain, but none is fully effective [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Campylobacter jejuni Biofilm Control with Lavandin Essential Oils and By-Products

et al. 2022

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The food industry is constantly struggling with one of the most prevalent biofilm-forming and food-borne pathogenic bacteria, Campylobacter jejuni. Different approaches are used to control biofilms in the food production chain, but none is fully effective. In this study, we aim to produce and determine the chemical profile of essential oils (EOs), ethanolic extracts of flowers prior to distillation (EFs), and ethanolic extracts of post-distillation waste material (EWMs) from Lavandula × intermedia ‘Bila’, ‘Budrovka’ St Nicholas and ‘Budrovka’, which were further used to reduce C. jejuni intercellular signaling, adhesion, and biofilm formation, as well as to test their antioxidant activity. Glycosides of hydroxycinnamic acids were the major constituents of both types of lavandin ethanolic extract, while linalool, linalyl acetate, 1,8-cineol, and camphor were the major compounds found in lavandin EOs. Tested EOs showed the best antibacterial activity with a minimal inhibitory concentration of 0.25 mg/mL. Lavandin EFs proved more effective in reducing C. jejuni intercellular signaling and adhesion compared to lavandin EOs and EWMs, while lavandin EOs showed a slightly better effect against biofilm formation. Interestingly, the best antioxidant activity was determined for lavandin EWMs. A positive and moderate correlation was found between the reduction of C. jejuni intercellular signaling and adhesion, as well as between adhesion and biofilm formation. These findings mean novel bacterial targets are of interest for biofilm control with alternative natural agents throughout the whole food production chain.

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Molecular Mechanisms of Campylobacter Biofilm Formation and Quorum Sensing

Cited by 19 publications

References 109 publications

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Rhodiola rosea Reduces Intercellular Signaling in Campylobacter jejuni

Campylobacter jejuni Biofilm Control with Lavandin Essential Oils and By-Products

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