Molecular mechanisms of developmentally programmed crinophagy in <i>Drosophila</i>

Csizmadia, Tamás; Lőrincz, Péter; Hegedüs, Katalin; Széplaki, Szilvia; Lőw, Péter; Juhász, Gábor

doi:10.1083/jcb.201702145

Cited by 63 publications

(106 citation statements)

References 53 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…However, many of the genes, including those discussed below, were tested with multiple RNAi lines targeting the same gene, showed phenotypes similar to other subunits of the same protein complex, or were verified with null mutants. These include genes encoding the SNAREs Syx16 and Snap29, which localize to both Golgi and endosomal compartments (Csizmadia et al, 2018;Morelli et al, 2014;Xu et al, 2002); the GTPase Rab5, which regulates trafficking at EE; and the ADP-ribosylation factor-like protein Arl1 and the Eps15 homology domain-containing protein 1 (EHD1) orthologue Past1, which are involved in EE-to-TGN retrograde trafficking ( Fig. 1, B-F; Christis and Munro, 2012;D'Souza et al, 2014;McKenzie et al, 2012;Sharma et al, 2009).…”

Section: Sg Maturation Requires Endocytic Contributionmentioning

confidence: 99%

An early endosome–derived retrograde trafficking pathway promotes secretory granule maturation

Cheng

Yang

Kim

et al. 2020

Journal of Cell Biology

View full text Add to dashboard Cite

Regulated secretion is a fundamental cellular process in which biologically active molecules stored in long-lasting secretory granules (SGs) are secreted in response to external stimuli. Many studies have described mechanisms responsible for biogenesis and secretion of SGs, but how SGs mature remains poorly understood. In a genetic screen, we discovered a large number of endolysosomal trafficking genes required for proper SG maturation, indicating that maturation of SGs might occur in a manner similar to lysosome-related organelles (LROs). CD63, a tetraspanin known to decorate LROs, also decorates SG membranes and facilitates SG maturation. Moreover, CD63-mediated SG maturation requires type II phosphatidylinositol 4 kinase (PI4KII)-dependent early endosomal sorting and accumulation of phosphatidylinositol 4-phosphate (PI4P) on SG membranes. In addition, the PI4P effector Past1 is needed for formation of stable PI4KII-containing endosomal tubules associated with this process. Our results reveal that maturation of post-Golgi–derived SGs requires trafficking via the endosomal system, similar to mechanisms employed by LROs.

show abstract

Section: Sg Maturation Requires Endocytic Contributionmentioning

confidence: 99%

An early endosome–derived retrograde trafficking pathway promotes secretory granule maturation

Cheng

Yang

Kim

et al. 2020

Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…Our model also provides a mechanistic and physiologic basis for the frequent fusion events between lysosomes and insulin granules in β‐cells previously described as crinophagy . The independence of crinophagy from autophagy was recently confirmed in the fly model . Of note, above findings do not exclude the possibility of delivery of insulin granules to lysosomes via canonical autophagy pathway—for example, upon occasional capture of granules (along with other cytosolic constituents) into autophagosomes.…”

Section: Lysosomes In Pancreatic β‐Cells: From Physiology To Diabetesmentioning

confidence: 80%

Lysosomes in nutrient signalling: A focus on pancreatic β‐cells

Mészáros

Pasquier

Vivot

et al. 2018

Diabetes Obesity Metabolism

View full text Add to dashboard Cite

Regulated insulin secretion from pancreatic β-cells is a major process maintaining glucose homeostasis in mammals. Enhancing insulin release in response to chronic nutrient overload and obesity-related insulin resistance (pre-diabetes) requires several adaptive cellular mechanisms maintaining β-cell health under such stresses. Once these mechanisms are overwhelmed, β-cell failure occurs leading to full-blown Type 2 Diabetes (T2D). Nutrient-dependent macroautophagy represents one such adaptive mechanism in β-cells. While macroautophagy levels are high and protective in β-cells in pre-diabetes, they decrease at later stages contributing to β-cell failure. However, mechanisms compromising macroautophagy in β-cells remain poorly understood. In this review, we discuss how recently discovered signalling cascades that emanate from the limiting membrane of lysosomes contribute to changes in macroautophagy flux in physiology and disease. In particular, these mechanisms are put into context with β-cell function highlighting most recently described links between nutrient-dependent lysosomal signalling pathways and insulin secretion. Understanding these mechanisms in response to metabolic stress might pave the way for development of more tailored treatment strategies aimed at preserving β-cell health.

show abstract

“…Interestingly there is evidence that ARFGEF3, like Rab2, has a role in secretory granule biogenesis (Buffa et al, 2008;Csizmadia et al, 2018;Sumakovic et al, 2009).…”

Section: Validation Of Novel Interaction Partners For Rab2a Rab5a Amentioning

confidence: 99%

In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation

Gillingham

Bertram

Begum

et al. 2019

Preprint

View full text Add to dashboard Cite

The GTPases of the Ras superfamily regulate cell growth, membrane traffic and the cytoskeleton, and a wide range of diseases are caused by mutations in particular members. They function as switchable landmarks with the active GTP-bound form recruiting to the membrane a specific set of effector proteins. The GTPases are precisely controlled by regulators that promote acquisition of GTP (GEFs) or its hydrolysis to GDP (GAPs). We report here MitoID, a method for identifying effectors and regulators by performing in vivo proximity biotinylation with mitochondriallylocalized forms of the GTPases. Applying this to 11 human Rab GTPases identified many known effectors and GAPs, as well as putative novel effectors, with examples of the latter validated for Rab2, Rab5 and Rab9. MitoID can also efficiently identify effectors and GAPs of Rho and Ras family GTPases such as Cdc42, RhoA, Rheb, and N-Ras, and can identify GEFs by use of GDP-bound forms.

show abstract

Molecular mechanisms of developmentally programmed crinophagy in Drosophila

Cited by 63 publications

References 53 publications

An early endosome–derived retrograde trafficking pathway promotes secretory granule maturation

An early endosome–derived retrograde trafficking pathway promotes secretory granule maturation

Lysosomes in nutrient signalling: A focus on pancreatic β‐cells

In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation

Contact Info

Product

Resources

About