Molecular mechanisms of EGF signaling-dependent regulation of pipe, a gene crucial for dorsoventral axis formation in Drosophila

Technau, Martin; Knispel, Meike; Roth, Siegfried

doi:10.1007/s00427-011-0384-2

Cited by 10 publications

(7 citation statements)

References 134 publications

(161 reference statements)

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“…Apart from aforementioned grk, pipe was also not expressed by P. aegeria . Pipe plays an essential role in establishing DV polarity in D. melanogaster oocytes, with its expression being confined to ventral follicle cells as a result of localised EGF signalling [91]. Recently, however, it has been proposed that pipe is not necessary in a number of insect species studied [4] and even in D. melanogaster there appears to be a second mechanism in establishing DV [92] that may involve delayed induction by graded maternal Dpp signalling in the perivitelline space [93].…”

Section: Discussionmentioning

confidence: 99%

“…Transcription factors that have been discussed as mediators of EGF signalling include pointed ( pnt ), aop and capicua ( cic ) [91]. Only the latter two were expressed by P. aegeria and present as maternal transcripts, but whether they play a role in establishing DV polarity remains to be investigated (Tables 6 and 7, and Additional file 2; qPCR results).…”

Section: Discussionmentioning

confidence: 99%

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Unscrambling butterfly oogenesis

Carter

Baker²,

Pink

et al. 2013

BMC Genomics

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BackgroundButterflies are popular model organisms to study physiological mechanisms underlying variability in oogenesis and egg provisioning in response to environmental conditions. Nothing is known, however, about; the developmental mechanisms governing butterfly oogenesis, how polarity in the oocyte is established, or which particular maternal effect genes regulate early embryogenesis. To gain insights into these developmental mechanisms and to identify the conserved and divergent aspects of butterfly oogenesis, we analysed a de novo ovarian transcriptome of the Speckled Wood butterfly Pararge aegeria (L.), and compared the results with known model organisms such as Drosophila melanogaster and Bombyx mori.ResultsA total of 17306 contigs were annotated, with 30% possibly novel or highly divergent sequences observed. Pararge aegeria females expressed 74.5% of the genes that are known to be essential for D. melanogaster oogenesis. We discuss the genes involved in all aspects of oogenesis, including vitellogenesis and choriogenesis, plus those implicated in hormonal control of oogenesis and transgenerational hormonal effects in great detail. Compared to other insects, a number of significant differences were observed in; the genes involved in stem cell maintenance and differentiation in the germarium, establishment of oocyte polarity, and in several aspects of maternal regulation of zygotic development.ConclusionsThis study provides valuable resources to investigate a number of divergent aspects of butterfly oogenesis requiring further research. In order to fully unscramble butterfly oogenesis, we also now also have the resources to investigate expression patterns of oogenesis genes under a range of environmental conditions, and to establish their function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Unscrambling butterfly oogenesis

Carter

Baker²,

Pink

et al. 2013

BMC Genomics

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“…How does the Torpedo signaling pathway repress pipe transcription? Analyses of the transcriptional regulatory elements controlling pipe expression have identified a stretch of approximately 50 nucleotides about 1.1 kb upstream of the transcriptional start site that is highly conserved in pipe genes from a number of Drosophila species . DNA fragments containing this element direct the transcription of a reporter gene in a pattern that is indistinguishable from that of pipe, and mutations introduced into the conserved 50 bp element lead to de‐repression of reporter gene expression in dorsal follicle cells.…”

Section: Dorsal–ventral Polarity In the Egg Chambermentioning

confidence: 99%

Maternal control of the Drosophila dorsal–ventral body axis

Stein

Stevens

2014

WIREs Developmental Biology

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The pathway that generates the dorsal–ventral (DV) axis of the Drosophila embryo has been the subject of intense investigation over the previous three decades. The initial asymmetric signal originates during oogenesis by the movement of the oocyte nucleus to an anterior corner of the oocyte, which establishes DV polarity within the follicle through signaling between Gurken, the Drosophila Transforming Growth Factor (TGF)-α homologue secreted from the oocyte, and the Drosophila Epidermal Growth Factor Receptor (EGFR) that is expressed by the follicular epithelium cells that envelop the oocyte. Follicle cells that are not exposed to Gurken follow a ventral fate and express Pipe, a sulfotransferase that enzymatically modifies components of the inner vitelline membrane layer of the eggshell, thereby transferring DV spatial information from the follicle to the egg. These ventrally sulfated eggshell proteins comprise a localized cue that directs the ventrally restricted formation of the active Spätzle ligand within the perivitelline space between the eggshell and the embryonic membrane. Spätzle activates Toll, a transmembrane receptor in the embryonic membrane. Transmission of the Toll signal into the embryo leads to the formation of a ventral-to-dorsal gradient of the transcription factor Dorsal within the nuclei of the syncytial blastoderm stage embryo. Dorsal controls the spatially specific expression of a large constellation of zygotic target genes, the Dorsal gene regulatory network, along the embryonic DV circumference. This article reviews classic studies and integrates them with the details of more recent work that has advanced our understanding of the complex pathway that establishes Drosophila embryo DV polarity.

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“…Two related papers on pipe regulation have appeared while this article was in revision (Technau et al, 2011;Fuchs et al, 2012). (Astigarraga et al, 2007;Cinnamon et al, 2008).…”

Section: Note Added In Proofmentioning

confidence: 99%

Mirror repressespipeexpression in follicle cells to initiate dorsoventral axis formation inDrosophila

et al. 2012

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SUMMARYDorsoventral (DV) axis formation in Drosophila begins with selective activation of EGFR, a receptor tyrosine kinase (RTK), in dorsal-anterior (DA) ovarian follicle cells. A critical event regulated by EGFR signaling is the repression of the sulfotransferaseencoding gene pipe in dorsal follicle cells, but how this occurs remains unclear. Here we show that Mirror (Mirr), a homeodomain transcription factor induced by EGFR signaling in DA follicle cells, directly represses pipe expression by binding to a conserved element in the pipe regulatory region. In addition, we find that the HMG-box protein Capicua (Cic) supports pipe expression in ventral follicle cells by repressing Mirr in this region. Interestingly, this role of Cic resembles its function in regulating anteroposterior (AP) body patterning, where Cic supports gap gene expression in central regions of the embryo by repressing Tailless, a repressor induced by RTK signaling at the embryonic poles. Thus, related RTK-Cic repressor circuits regulate the early stages of Drosophila DV and AP body axis formation.

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Molecular mechanisms of EGF signaling-dependent regulation of pipe, a gene crucial for dorsoventral axis formation in Drosophila

Cited by 10 publications

References 134 publications

Unscrambling butterfly oogenesis

Unscrambling butterfly oogenesis

Maternal control of the Drosophila dorsal–ventral body axis

Mirror repressespipeexpression in follicle cells to initiate dorsoventral axis formation inDrosophila

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