Molecular Mechanisms of Pituitary Cell Plasticity

Childs, Gwen V.; MacNicol, Angus M.; MacNicol, Melanie C.

doi:10.3389/fendo.2020.00656

Cited by 21 publications

(13 citation statements)

References 53 publications

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“…The presence of cells expressing more than one hormone in the anterior pituitary has been shown in many studies, both in teleosts and in mammals [for review see (27,(74)(75)(76)]. Using scRNA-seq technology, multi-hormonal cells have been described in the mouse pituitary (77), but never before in a teleost.…”

Section: Scrna-seq and Multi-color Fish Reveal The Presence Of Multi-hormonal Cells In The Adult Medaka Pituitarymentioning

confidence: 99%

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

et al. 2021

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In vertebrates, the anterior pituitary plays a crucial role in regulating several essential physiological processes via the secretion of at least seven peptide hormones by different endocrine cell types. Comparative and comprehensive knowledge of the spatial distribution of those endocrine cell types is required to better understand their physiological functions. Using medaka as a model and several combinations of multi-color fluorescence in situ hybridization, we present the first 3D atlas revealing the gland-wide distribution of seven endocrine cell populations: lactotropes, thyrotropes, Lh and Fsh gonadotropes, somatotropes, and pomca-expressing cells (corticotropes and melanotropes) in the anterior pituitary of a teleost fish. By combining in situ hybridization and immunofluorescence techniques, we deciphered the location of corticotropes and melanotropes within the pomca-expressing cell population. The 3D localization approach reveals sexual dimorphism of tshba-, pomca-, and lhb-expressing cells in the adult medaka pituitary. Finally, we show the existence of bi-hormonal cells co-expressing lhb-fshb, fshb-tshba and lhb-sl using single-cell transcriptomics analysis and in situ hybridization. This study offers a solid basis for future comparative studies of the teleost pituitary and its functional plasticity.

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Section: Scrna-seq and Multi-color Fish Reveal The Presence Of Multi-hormonal Cells In The Adult Medaka Pituitarymentioning

confidence: 99%

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In all fish, LH and FSH are produced by different cells, whereas in most tetrapods they seem to be produced in the same cell ( Siddique et al, 2020 ). There are lines of evidence also showing that one pituitary cell type expressing multiple hormone mRNAs exists in normal rodent pituitaries ( Childs et al, 2000 ; Seuntjens et al, 2002 ; Villalobos et al, 2004 ; Childs et al, 2020 ). In chicken pituitaries, we found that lactotrophs predominantly express PRL , however, they can also weakly express LHB, GRP, GH, POMC , and TSHB .…”

Section: Resultsmentioning

confidence: 99%

Single-Cell RNA Sequencing Analysis of Chicken Anterior Pituitary: A Bird’s-Eye View on Vertebrate Pituitary

Zhang

et al. 2021

Front. Physiol.

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It is well-established that anterior pituitary contains multiple endocrine cell populations, and each of them can secrete one/two hormone(s) to regulate vital physiological processes of vertebrates. However, the gene expression profiles of each pituitary cell population remains poorly characterized in most vertebrate groups. Here we analyzed the transcriptome of each cell population in adult chicken anterior pituitaries using single-cell RNA sequencing technology. The results showed that: (1) four out of five known endocrine cell clusters have been identified and designated as the lactotrophs, thyrotrophs, corticotrophs, and gonadotrophs, respectively. Somatotrophs were not analyzed in the current study. Each cell cluster can express at least one known endocrine hormone, and novel marker genes (e.g., CD24 and HSPB1 in lactotrophs, NPBWR2 and NDRG1 in corticotrophs; DIO2 and SOUL in thyrotrophs, C5H11ORF96 and HPGDS in gonadotrophs) are identified. Interestingly, gonadotrophs were shown to abundantly express five peptide hormones: FSH, LH, GRP, CART and RLN3; (2) four non-endocrine/secretory cell types, including endothelial cells (expressing IGFBP7 and CFD) and folliculo-stellate cells (FS-cells, expressing S100A6 and S100A10), were identified in chicken anterior pituitaries. Among them, FS-cells can express many growth factors, peptides (e.g., WNT5A, HBEGF, Activins, VEGFC, NPY, and BMP4), and progenitor/stem cell-associated genes (e.g., Notch signaling components, CDH1), implying that the FS-cell cluster may act as a paracrine/autocrine signaling center and enrich pituitary progenitor/stem cells; (3) sexually dimorphic expression of many genes were identified in most cell clusters, including gonadotrophs and lactotrophs. Taken together, our data provides a bird’s-eye view on the diverse aspects of anterior pituitaries, including cell composition, heterogeneity, cell-to-cell communication, and gene expression profiles, which facilitates our comprehensive understanding of vertebrate pituitary biology.

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“…The presence of cells expressing more than one hormone in the anterior pituitary has been shown in many studies, both in teleosts and in mammals (for review see (28,(69)(70)(71)). Using scRNA-seq technology, multi-hormonal cells have for instance been described in the mouse pituitary (72).…”

Section: Scrna-seq and Multi-color Fish Reveal The Presence Of Multi-hormonal Cells In The Adult Medaka Pituitarymentioning

confidence: 97%

3D atlas of the pituitary gland of the model fish medaka

Royan

Siddique

Csúcs

et al. 2021

Preprint

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In vertebrates, the anterior pituitary plays a crucial role in regulating several essential physiological processes via the secretion of at least seven peptide hormones by different endocrine cell types. Comparative and comprehensive knowledge of the spatial distribution of those endocrine cell types is required to better understand their role during the animal life. Using medaka as the model and several combinations of multi-color fluorescence in situ hybridization, we present the first 3D atlas revealing the gland-wide distribution of seven endocrine cell populations: lactotropes, thyrotropes, Lh and Fsh gonadotropes, somatotropes, and pomca-expressing cells (corticotropes and melanotropes) in the anterior pituitary of a teleost fish. By combining in situ hybridization and immunofluorescence techniques, we deciphered the location of corticotropes and melanotropes within the pomca-expressing cell population. The 3D localization approach reveals sexual dimorphism of tshba- and pomca-expressing cells in the adult medaka pituitary. Finally, we show the existence of bi-hormonal cells co-expressing lhb-fshb, fshb-tshba and lhb-sl using single-cell transcriptomics analysis and in situ hybridization. This study offers a solid basis for future comparative studies of the teleost pituitary and its developmental plasticity.

show abstract

Molecular Mechanisms of Pituitary Cell Plasticity

Cited by 21 publications

References 53 publications

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes)

Single-Cell RNA Sequencing Analysis of Chicken Anterior Pituitary: A Bird’s-Eye View on Vertebrate Pituitary

3D atlas of the pituitary gland of the model fish medaka

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