Molecular Mechanisms of Renal Ischemic Conditioning Strategies

Kierulf-Lassen, Casper; Nieuwenhuijs-Moeke, Gertrude J.; Krogstrup, Nicoline V.; Oltean, Mihai; Jespersen, Bente; Dor, Frank J. M. F.

doi:10.1159/000437352

Cited by 25 publications

(20 citation statements)

References 165 publications

(330 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The bar diagram shows average fractional fibrosis in control (sham, n=16), untreated ischemia (saline, n=20), ischemia treated with normoxic EV (EXO, n=20), and ischemia treated with IPC EV (IPC EXO, n=20). Sham and saline were significantly different (P=2.94310 26 ), sham and EXO were also significantly different (P,0.01), but sham and IPC EXO were not (P=0.08). In addition, EXO (P,0.001) and IPC EXO (P=3.31310 26 ) were significantly lower than saline, and IPC EXO was significantly lower than EXO (P,0.02).…”

Section: Mrna Pathwaysmentioning

confidence: 93%

Renal Tubular Cell-Derived Extracellular Vesicles Accelerate the Recovery of Established Renal Ischemia Reperfusion Injury

Dominguez

Liu

Gao

et al. 2017

JASN

View full text Add to dashboard Cite

Ischemic renal injury is a complex syndrome; multiple cellular abnormalities cause accelerating cycles of inflammation, cellular damage, and sustained local ischemia. There is no single therapy that effectively resolves the renal damage after ischemia. However, infusions of normal adult rat renal cells have been a successful therapy in several rat renal failure models. The sustained broad renal benefit achieved by relatively few donor cells led to the hypothesis that extracellular vesicles (EV, largely exosomes) derived from these cells are the therapeutic effector We now show that EV from adult rat renal tubular cells significantly improved renal function when administered intravenously 24 and 48 hours after renal ischemia in rats. Additionally, EV treatment significantly improved renal tubular damage, 4-hydroxynanoneal adduct formation, neutrophil infiltration, fibrosis, and microvascular pruning. EV therapy also markedly reduced the large renal transcriptome drift observed after ischemia. These data show the potential utility of EV to limit severe renal ischemic injury after the occurrence.

show abstract

Section: Mrna Pathwaysmentioning

confidence: 93%

Renal Tubular Cell-Derived Extracellular Vesicles Accelerate the Recovery of Established Renal Ischemia Reperfusion Injury

Dominguez

Liu

Gao

et al. 2017

JASN

View full text Add to dashboard Cite

show abstract

“…In addition to strategies for reconditioning of donor organs using machine perfusion, other innovations to combat ischaemia–reperfusion injury are currently being evaluated in randomized clinical trials, such as ischaemic conditioning, pharmacological conditioning (using complement regulatory drugs or calcineurin inhibitors) and cell‐based therapies in recipients (ONE study).…”

Section: Future Developmentsmentioning

confidence: 99%

Kidney and liver transplantation in the elderly

Sutherland

IJzermans

Forsythe

et al. 2016

Journal of British Surgery

View full text Add to dashboard Cite

Background: Transplant surgery is facing a shortage of deceased donor organs. In response, the criteria for organ donation have been extended, and an increasing number of organs from older donors are being used. For recipients, the benefits of transplantation are great, and the growing ageing population has led to increasing numbers of elderly patients being accepted for transplantation. Methods:The literature was reviewed to investigate the impact of age of donors and recipients in abdominal organ transplantation, and to highlight aspects of the fine balance in donor and recipient selection and screening, as well as allocation policies fair to young and old alike.Results: Overall, kidney and liver transplantation from older deceased donors have good outcomes, but are not as good as those from younger donors. Careful donor selection based on risk indices, and potentially biomarkers, special allocation schemes to match elderly donors with elderly recipients, and vigorous recipient selection, allows good outcomes with increasing age of both donors and recipients. The results of live kidney donation have been excellent for donor and recipient, and there is a trend towards inclusion of older donors. Future strategies, including personalized immunosuppression for older recipients as well as machine preservation and reconditioning of donor organs, are promising ways to improve the outcome of transplantation between older donors and older recipients.Conclusion: Kidney and liver transplantation in the elderly is a clinical reality. Outcomes are good, but can be optimized by using strategies that modify donor risk factors and recipient co-morbidities, and personalized approaches to organ allocation and immunosuppression.

show abstract

“…It was known that short periods of intermittent ischemia and reperfusion can trigger ischemic conditioning that is an inherent piece of the renal defense mechanism against ischemia. So a great number of studies are necessary to understand the signalling transduction pathways of renal ischemic conditioning (24).…”

Section: Discussionmentioning

confidence: 99%

Investigation of argyrophilic nucleolar organizing region

Eröz¹,

Nisari²,

Ertekın³

et al. 2016

BLL

View full text Add to dashboard Cite

BACKGROUND: Ischemia/reperfusion (I/R) injury is a complex event frequently observed in vascular surgery and can cause functional and structural cell damage. Nucleolar-organizing regions (NORs) are sites of the ribosomal genes located on chromosomes and can be stained with silver when they are active. Thus these proteins are named as argyrophilic-NOR (AgNOR)-associated proteins. We aimed to investigate any possible effects of renal I/R injury on the NOR protein synthesis and association between the AgNOR proteins amount and histopathological injuring score. METHODS: Nine female wistar-albino rats with weight of 200-250g were included into the study. The animals were randomly divided in two groups, a Control Group and an I/R Group. In I/R group, rats were subjected to 45 minutes of renal pedicle occlusion followed by 24 hours of reperfusion. In the control group no drug injections or ischemia reperfusion were performed in animals. Then histopathological injury score, mean AgNOR number and total AgNOR area/nuclear area (TAA/NA) were detected for each rat. RESULTS: The differences between control and I/R groups were signifi cant for histopathological injury scores (p = 0.016). Also the differences between control group and I/R group were signifi cant for mean AgNOR number (p = 0.000) and TAA/NA ratio (p = 0.000). Additionally, there was a positive correlation between TAA/NA ratio and histopathological injury score (r = 0.728; p = 0.026) and between mean AgNOR number and histopathological injury score (r = 0.670; p = 0.048). CONCLUSION: The detection of AgNOR proteins amount may be used as an indicator to obtain information about the cellular behaviour (self-protective mechanism of tubular epithelial cells) against I/R injury and cellular damage levels (Tab. 2, Fig. 4, Ref. 24). Text in PDF www.elis.sk.

show abstract

Molecular Mechanisms of Renal Ischemic Conditioning Strategies

Cited by 25 publications

References 165 publications

Renal Tubular Cell-Derived Extracellular Vesicles Accelerate the Recovery of Established Renal Ischemia Reperfusion Injury

Renal Tubular Cell-Derived Extracellular Vesicles Accelerate the Recovery of Established Renal Ischemia Reperfusion Injury

Kidney and liver transplantation in the elderly

Investigation of argyrophilic nucleolar organizing region

Contact Info

Product

Resources

About