Molecular mechanisms of resistance to spirodiclofen and spiromesifen in Tetranychus urticae

İnak, Emre; Demirci, Berke; Vandenhole, Marilou; Söylemezoğlu, Gökhan; Leeuwen, Thomas Van; Toprak, Umut

doi:10.1016/j.cropro.2023.106343

Cited by 8 publications

(3 citation statements)

References 53 publications

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“…Della Vechia et al (2021) reported that Brevipalpus californicus (Banks) exposed to the insecticide imidacloprid alone or in combination with spirodiclofen showed an increase in the activity of P450s, carboxylcholinesterases (CCE), and glutathione-S-transferases (GST). There are many publications on the resistance mechanisms of abamectin or spirodiclofen selected populations in different pests (Dermauw et al 2012;Çağatay et al 2018;Badieinia et al 2020;İnak et al 2023). However, the resistance mechanism of pests selected by applying mixed pesticide formulations has yet to be explored.…”

Section: Discussionmentioning

confidence: 99%

“…We previously determined that 1199-fold resistance developed against the S+A mixture formulation in the susceptible population of T. urticae that was selected with this mixture formulation (Alsay & Ay 2022). Although here are many studies on the resistance mechanism of spiromesifen and abamectin in T. urticae separately (Stumpf & Nauen 2002;Demaeght et al 2013;Solmaz et al 2020;İnak et al 2022;İnak et al 2023), there are limited studies on how pesticide mixture formulations cause changes in the physiology of resistant pests. It is known that in some cases, the activity of detoxification enzymes increases in abamectin-resistant T. urticae populations (Kwon et al 2010a;Riga et al 2014;Çağatay et al 2018a) and in other cases target site mutations on the glutamate-gated chloride channels (G314D, G326E, I321T) are involved in the resistance (Kwon et al 2010b;Dermauw et al 2012;Ilias et al 2017;Xue et al 2020Xue et al , 2021.…”

Section: Introductionmentioning

confidence: 99%

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The roles of the biochemical and molecular changes in resistance of Tetranychus urticae populations selected with spiromesifen+abamectin mixture </p >

Ay,

Çevik,

Alsay

2024

saa

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The physiological changes in Tetranychus urticae selected with spiromesifen+abamectin (S+A) mixture formulation were demonstrated by biochemical and molecular methods. The susceptible (GSS) population of T. urticae, was selected with S+A and made resistant with increasing doses of the mixture formulation. The resistant population was divided into two, and selection pressure was terminated in one population which is named IR (Interrupt resistant). In the other population, the selection with S+A was continued and the population named IR2. Activities of some detoxification enzymes (esterase, glutathione-S-transferase (GST) and cytochrome P450) were investigated in the GSS and IR populations. The activity of esterase, GST and P450 enzymes were increased by 1.77, 2.57 and 2.58-fold, respectively, in the IR population compared to the GSS population. Also, investigation of synergistic effects with esterase inhibitor (TTP), GST inhibitor (DEM) and P450 inhibitor (PBO) in this population revealed that all three synergists showed significant synergistic effects in IR population. In molecular studies, when the population was screened for the presence of two previously identified glutamate channel mutations (G314D and G326E) by qPCR with TaqMan probes no mutations could be detected in glutamate channels. and the CTD domain of acetyl coenzyme-A carboxylase (ACCase). Furthermore, possible genetic mutations in the biotin carboxylase domain (BCD) and carboxyl transferase domain (CTD) of the ACCase target sites were determined by sequencing. Although a single amino acid mutation G37D in the BCD of the ACCase gene of the IR and IR2 populations was detected, its association with spiromesifen resistance was not confirmed. The results indicated that increased detoxification and possible target site mutation may be responsible for the S+A resistance in the IR and IR2 populations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The roles of the biochemical and molecular changes in resistance of Tetranychus urticae populations selected with spiromesifen+abamectin mixture </p >

Ay,

Çevik,

Alsay

2024

saa

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“…Indeed, attempts to introduce the mutation in Drosophila using CRISPR/Cas9 genome editing failed as it turned out to be homozygous lethal [ 32 ]. Metabolic resistance mechanisms driven by detoxification enzymes such as cytochrome-P450 monooxygenases (P450s), glutathione S-transferases, carboxylesterases, and UDP-glycosyltransferases have not yet been described in whiteflies [ 37 ], but these have been shown to play a major role in spider mites, e.g., Tetranychus urticae , targeted by ketoenol insecticides [ 38 , 39 ]. Despite increasing selection pressure no cases of insecticide resistance have yet been described in A. proletella , except for pyrethroids in the UK [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Mapping and Characterization of Target-Site Resistance to Cyclic Ketoenol Insecticides in Cabbage Whiteflies, Aleyrodes proletella (Hemiptera: Aleyrodidae)

Müller,

Maiwald,

Lange

et al. 2024

Insects

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Cabbage whitefly, Aleyrodes proletella L., is an invasive hemipteran pest of cruciferous plants, particularly field brassica crops. Its importance has been increased over the last decade, particularly in European countries. The control of cabbage whiteflies largely relies on the application of synthetic insecticides, including tetronic and tetramic acid derivatives such as spiromesifen and spirotetramat (cyclic ketoenol insecticides), acting as insect growth regulators targeting acetyl-CoA carboxylase (ACC). In 2019, reduced efficacy against cabbage whiteflies of ketoenol insecticides at recommended label rates has been reported. Subsequently we collected field samples of A. proletella in different European countries and confirmed the presence of ketoenol resistance in laboratory bioassays. Reciprocal crossing experiments revealed an autosomal dominant trait, i.e., heterozygotes express a fully resistant phenotype. Transcriptome sequencing and assembly of ACC variants from resistant strains revealed the presence of an ACC target-site mutation, A2083V, as previously described and functionally validated in Bemisia tabaci (A2084V in A. proletella). Next, we employed a molecular genotyping assay to investigate the geographic spread of resistance and analyzed 49 populations collected in eight European countries. Resistance allele frequency was highest in the Netherlands, followed by Germany. Finally, we provide a proposal for the implementation of appropriate resistance management strategies.

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Lipid Metabolism as a Target Site in Pest Control

Toprak,

İnak,

Nauen

2024

Advances in Experimental Medicine and Biology

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Molecular mechanisms of resistance to spirodiclofen and spiromesifen in Tetranychus urticae

Cited by 8 publications

References 53 publications

The roles of the biochemical and molecular changes in resistance of Tetranychus urticae populations selected with spiromesifen+abamectin mixture </p >

The roles of the biochemical and molecular changes in resistance of Tetranychus urticae populations selected with spiromesifen+abamectin mixture </p >

Mapping and Characterization of Target-Site Resistance to Cyclic Ketoenol Insecticides in Cabbage Whiteflies, Aleyrodes proletella (Hemiptera: Aleyrodidae)

Lipid Metabolism as a Target Site in Pest Control

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