Molecular Mechanisms Underlying the Role of MicroRNAs in the Chemoresistance of Pancreatic Cancer

Garajová, Ingrid; Large, Tessa Y. Le; Frampton, Adam E.; Rolfo, Christian; Voortman, Johannes; Giovannetti, Elisa

doi:10.1155/2014/678401

Cited by 41 publications

(45 citation statements)

References 179 publications

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“…Chemoresistance is one of the main obstacles that limit the therapeutic effects of drugs, but the mechanisms that contribute to this have not been fully elucidated. Consistent with previous findings [19,20], we found that miR-34a influences the response of PDAC cells to 5-FU. Our study suggests that the loss of miR-34a expression may be associated with the chemoresistance of PDAC tumor cells.…”

Section: Discussionsupporting

confidence: 93%

The Clinical Significance of miR-34a in Pancreatic Ductal Carcinoma and Associated Molecular and Cellular Mechanisms

et al. 2016

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Background: Pancreatic ductaladenocarcinoma (PDAC) exhibits poor prognosis and resistance to chemotherapy. This study was to identify the biomarkers associated with the progression, poor prognosis and chemoresistance of PDAC. Methods: miR-34a and miR-150 levels in the plasma and tissues from PDAC patients were measured by real-time PCR. Xenograft PDAC tumor models were established in mice by inoculation of CD133+ stem cells isolated from PDAC tumors. Protein expression was measured by Western blot. Results: The plasma miR-34a and miR-150 levels were significantly lower in PDAC patients than in patients with benign pancreatic lesions and in healthy subjects. The miR-34a and miR-150 levels in the tumor tissues were significantly lower than in pancreatic tissues with benign lesions. The protein levels of CD133, Notch1, Notch2 and Notch4 receptors in PDAC tumor tissues were significantly higher than in pancreatic tissues with benign lesions. miR-34a injection significantly inhibited the tumor growth of PDAC tumors and sensitized the anticancer effects of 5-fluorouracil (5-FU). miR-34a significantly inhibited Notch1, Notch2 and Notch4 expression in xenograft tumor tissues in vivo and BxPC-3 cells in vitro. miR-34a and miR-150 significantly induced apoptosis and inhibited proliferation, invasion and migration in BxPC-3 cells. miR-34a, but not miR-150, significantly sensitized the anticancer effect of 5-FU in BxPC-3 cells in vitro. Conclusion: A loss of expression of miR-34a, but not of miR-150, is associated with disease progression and poor prognosis in PDAC patients, and may be involved in the chemoresistance of PDAC cells.

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Section: Discussionsupporting

confidence: 93%

The Clinical Significance of miR-34a in Pancreatic Ductal Carcinoma and Associated Molecular and Cellular Mechanisms

et al. 2016

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“…In general, miRNAs are composed of 19-25 nucleotides. Science magazine considered the discovery of miRNA to be one of the top ten scientific advancements in 2002and 2003(Garajova et al, 2014Luo et al, 2014). The relationship between miRNAs and tumors was first identified in chronic lymphocytic leukemia (CLL).…”

Section: Discussionmentioning

confidence: 99%

“…The pathogenesis of osteosarcoma, including development and metastasis, involves multiple genes (Garajova et al, 2014;Phuah and Nagoor, 2014). MicroRNAs (miRNAs), which widely exist in animals and plants, are non-coding, small, regulatory RNAs (Orang and Barzegari, 2014;Saadatian et al, 2014) that can negatively regulate gene expression.…”

Section: Introductionmentioning

confidence: 99%

Regulatory role of microRNA184 in osteosarcoma cells

Gr¹,

Wang²,

Xb³

et al. 2015

Genet. Mol. Res.

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“…Increasingly, previous studies have focused on the inhibitory effects of miRs on cancer and tumors; cyclooxygenase-2 activity was demonstrated to be suppressed by miR-143, preventing gastric cancer growth and inducing the apoptosis of gastric cancer cells (34). Furthermore, miR-145 levels were enhanced by histone deacetylase inhibitors in a Burkitt lymphoma cell line, and the levels of miR-143 were markedly downregulated in samples from patients with Burkitt lymphoma (35).…”

Section: Discussionmentioning

confidence: 99%

miR-143 suppresses the proliferation of NSCLC cells by inhibiting the epidermal growth factor receptor

Zhang

Sun

Ling

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. MicroRNAs (miRs) regulate the proliferation and metastasis of numerous cancer cell types. It was previously reported that miR-143 levels were downregulated in non-small cell lung cancer (NSCLC) tissues and cell lines, and that the migration and invasion of NSCLC cells was inhibited upon suppression of cell proliferation and colony formation by the upregulation of miR-143. Epidermal growth factor receptor (EGFR), which is a vital factor in the promotion of cancer cell proliferation and has been investigated as a potential focus in cancer therapy, has been reported to be a possible target of miR-143. The present study aimed to investigate the role of miR-143 in NSCLC using NSCLC cell lines and primary cells from NSCLC patients. NSCLC cells were co-transfected with EGFR and miR-143, and the mRNA and protein expression of EGFR were analyzed. Furthermore, the activity of the transfected cancer cells with regard to colony formation, migration, invasion and apoptosis were evaluated. The levels of miR-143 were decreased in the NSCLC cell lines and primary cells from patients with NSCLC compared with the controls. Following transfection with miR-143, the ability of NSCLC cells to proliferate, form colonies, migrate and invade was inhibited. Similarly, knockdown of EGFR led to the suppression of NSCLC cell proliferation. The mRNA and protein expression levels of EGFR were significantly reduced following miR-143 overexpression, and the level of miR-143 was inversely correlated with that of EGFR in NSCLC cells. The results of the present study demonstrated that miR-143 was able to suppress NSCLC cell proliferation and invasion by inhibiting the effects of EGFR, suggesting that EGFR may be considered a potential target for NSCLC therapy.

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Molecular Mechanisms Underlying the Role of MicroRNAs in the Chemoresistance of Pancreatic Cancer

Cited by 41 publications

References 179 publications

The Clinical Significance of miR-34a in Pancreatic Ductal Carcinoma and Associated Molecular and Cellular Mechanisms

The Clinical Significance of miR-34a in Pancreatic Ductal Carcinoma and Associated Molecular and Cellular Mechanisms

Regulatory role of microRNA184 in osteosarcoma cells

miR-143 suppresses the proliferation of NSCLC cells by inhibiting the epidermal growth factor receptor

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