Molecular Method for the Detection of Resistance Aspergillus Fumigatus to Some Antifungal Agents

Abstract

Twenty samples were obtained from Aspergillus fumigatus, out of a total of 45 phlegm samples 41 samples were positive for A. fumigatus patients aged (33 to 82 years of both sexes), from August 2022 to December 2022 from the Specialist Center Samples were collected from several Iraqi governorates from the north, center and south. The results indicated that the isolation of AFU1, AFU12 and AFU31 was the highest resistance to the antihistamines. Anti-fungal resistance, most of which have allergic reactions to these antibiotics in a clear reference to the ability of these molds to adapt to the ocean up this adaptation to the occurrence of genetic mutations Genetic diversity and relationships were studied using the RAPD technique. It produced four primers (111) major pandas, including 9 unique pandas, common pandas (14) and polymorphic forests. Inflated ranges ranged from 100 to 2,000 basis points. The value of the genetic polymorphism of each primer ranged between 33-100%. In terms of unique band patterns, the most characteristic band pattern for the number isolated with the primer OP-M06 and OP-R06 was given 37 pandas of which 3 were unique in each. Genetic distances ranged from 0.22805 to 0.66905 between A. fumigatus isolates. Cluster analyzes were conducted to construct a dendrogram tree showing the interconnection of isolates. Most of the isolated A. fumigatus isolates from the patient developed come from the same region (conservative) in close relationship (sub-mass) indicating a relationship between RAPD patterns and origin of isolates.