2014
DOI: 10.7589/2013-09-231
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MOLECULAR METHODS TO DETECTMYCOPLASMASPP. AND TESTUDINID HERPESVIRUS 2 IN DESERT TORTOISES (GOPHERUS AGASSIZII) AND IMPLICATIONS FOR DISEASE MANAGEMENT

Abstract: ABSTRACT:Mycoplasmas are an important cause of upper respiratory tract disease (URTD) in desert tortoises (Gopherus agassizii) and have been a main focus in attempts to mitigate diseasebased population declines. Infection risk can vary with an animal's population of origin, making screening tests popular tools for determining infection status in individuals and populations. To provide additional methods for investigating URTD we developed quantitative PCR (qPCR) assays specific for agents causing clinical sign… Show more

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Cited by 39 publications
(44 citation statements)
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“…Thus, it appears that recrudescence is as important a process as transmission in introducing disease into a seemingly naïve population. One important caveat to interpretations from this experiment is that sensitive genetic tests, namely quantitative PCR, were not yet available to detect M. agassizii in seronegative, healthy‐appearing individuals (Braun et al., ; Sandmeier et al., ). Explicit information about the presence of the pathogen would have allowed for additional analyses on how pathogen load may be involved in recrudescence.…”
Section: Discussionmentioning
confidence: 99%
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“…Thus, it appears that recrudescence is as important a process as transmission in introducing disease into a seemingly naïve population. One important caveat to interpretations from this experiment is that sensitive genetic tests, namely quantitative PCR, were not yet available to detect M. agassizii in seronegative, healthy‐appearing individuals (Braun et al., ; Sandmeier et al., ). Explicit information about the presence of the pathogen would have allowed for additional analyses on how pathogen load may be involved in recrudescence.…”
Section: Discussionmentioning
confidence: 99%
“…Animals were examined for visible signs of URTD monthly. URTD was defined as any indication of past or present discharge of mucous from the nares: partially or fully occluded nares, moisture or caked soil around the nares, and visible or external exudate (Braun et al., ; Sandmeier et al. 2017).…”
Section: Methodsmentioning
confidence: 99%
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“…Oral swabs (1 per assessment) and nasal lavage samples were shipped on dry ice to the San Diego Zoo Amphibian Disease Lab (Escondido, CA, USA) for quantitative Polymerase Chain Reaction (qPCR) to detect and estimate abundance of M. agassizii (Braun et al . ). Results for each qPCR test could be negative, positive or equivocal (inconclusive).…”
Section: Methodsmentioning
confidence: 97%
“…Seroprevalence is a poor indicator of prevalence of the microbe M. agassizii , but quantitative PCR (qPCR) can detect very low levels of the pathogen within tortoises (Braun et al. , Sandmeier et al. ).…”
Section: Introductionmentioning
confidence: 99%