Molecular mobility in the cytoplasm: An approach to describe and predict lifespan of dry germplasm

Buitink, Julia; Leprince, Olivier; Hemminga, M.A.; Hoekstra, Folkert A.

doi:10.1073/pnas.040554797

Cited by 84 publications

(61 citation statements)

References 46 publications

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“…As reported by Hoekstra et al (2001), when seed WC is about 10% (0.11 g g 21 ), the cytoplasm in dehydrated cells is subject to vitrification and forms an amorphous glass that resembles the solid state, but retains the physical properties of a liquid. In this state, the diffusion of molecules and the rate of chemical reactions are greatly retarded, which prolongs the potential lifespan of stored seeds (Buitink et al 2000;Walters et al 2004.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

Chmielarz

2009

Acta Physiol Plant

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To evaluate the feasibility of long-term cryopreservation of Polish provenances of silver birch (Betula pendula), the sensitivity of conditionally dormant seeds to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196°C) was evaluated. The critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or for 2 years in LN was also determined. Germination tests revealed no critical WC for seeds [to 0.02 g H 2 O g -1 dry mass (g g -1 )]. Seeds tolerated freezing in LN within specific safe range of WC 0.02-0.23 g g -1 (nuts). Seeds desiccated to the safe WC and stored in LN for 2 years had similar or higher germination as seeds stored at -3°C for 2 years, depending on provenance. Therefore, long-term cryopreservation of B. pendula seeds in gene banks is feasible.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

Chmielarz

2009

Acta Physiol Plant

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“…the position as which the molecular constancy of glasses is almost nil, as a pointer of molecular mobility. Walters (2004) Even though the time scales evaluated by Buitink et al (2000) and Walters (2004) are different from that used in our experiment (years in their case, days in ours), their results can be used to elucidate some of our results. The molecular mobility happening in the cytoplasm for the duration of cryostorage might, for example, provoke oxidative stress throughout the development of free radicals, which may injure the cell membrane organization and create microfractures on it.…”

Section: Resultsmentioning

confidence: 61%

Short-Term Liquid Nitrogen Storage of Maize, Common Bean and Soybean Seeds Modiﬁes Their Biochemical Composition

Arguedas¹,

Pérez²,

Abdelnour³

et al. 2016

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We studied the effects of liquid nitrogen storage of maize, common bean and soybean seeds on their germination, electrolyte leakage, levels of chlorophylls, phenolics, aldehydes, proteins and peroxidase activity. After storage for 28 days, seeds were retrieved from liquid nitrogen, some were set to germinate and others were analyzed biochemically. No phenotypic modifications were observed visually 5 days after beginning of germination, although percentage of seed germination was reduced by LN in maize and soybean. Moreover, numerous significant effects of seed cryopreservation were recorded at the biochemical status. In maize seeds, the most important and statistically significant modifications were observed in the increased levels of chlorophyll b and total chlorophyll pigments and in the decreased contents of free phenolics after 28 days of exposure to LN, compared to the control treatment. In common bean, relevant changes were observed in the increased electrolyte leakage and in the reduced levels of chlorophyll pigments (b, total) and free phenolics. In soybean, modifications were observed in the increased levels of chlorophyll pigments (a, b, total), malondialdehyde and electrolyte leakage, and in the decreased peroxidase activity. We have shown for the first time that immersion of maize, common bean and soybean seeds in liquid nitrogen modified the levels of different biochemicals.

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“…These relationships were initially noted by observations that the magnitude of DH sorp was low (close to 0) in desiccationintolerant tissues (Vertucci and Leopold, 1987b;Vertucci et al, 1994;Eira et al, 1999) and that DH sorp(w) could be used as a first approximation of viscosity in seeds (Vertucci and Roos, 1990). Studies subsequent to these showed that changes in viscosity were linked to glass transitions and corresponded to changes in ageing kinetics (Leopold et al, 1994;Buitink et al, 1998Buitink et al, , 2000Walters, 2004). Building on the idea that the magnitude and change of DH sorp reflects structural restrictions and relaxations within a glass, it can be hypothesized that high values of c in the BET model or K in the D'Arcy-Watt model reflect a condition of high molecular stability.…”

Section: Discussionmentioning

confidence: 92%

Water properties in fern spores: sorption characteristics relating to water affinity, glassy states, and storage stability

Ballesteros

Walters²

2007

Journal of Experimental Botany

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Ex situ conservation of ferns may be accomplished by maintaining the viability of stored spores for many years. Storage conditions that maximize spore longevity can be inferred from an understanding of the behaviour of water within fern spores. Water sorption properties were measured in spores of five homosporeous species of ferns and compared with properties of pollen, seeds, and fern leaf tissue. Isotherms were constructed at 5, 25, and 45°C and analysed using different physicochemical models in order to quantify chemical affinity and heat (enthalpy) of sorption of water in fern spores. Fern spores hydrate slowly but dry rapidly at ambient relative humidity. Low Brunauer-Emmet-Teller monolayer values, few water-binding sites according to the D'Arcy-Watt model, and limited solute-solvent compatibility according to the Flory-Huggins model suggest that fern spores have low affinity for water. Despite the low water affinity, fern spores demonstrate relatively high values of sorption enthalpy (DH sorp ). Parameters associated with binding sites and DH sorp decrease with increasing temperature, suggesting temperature-and hydration-dependent changes in volume of spore macromolecules. Collectively, these data may relate to the degree to which cellular structures within fern spores are stabilized during drying and cooling. Water sorption properties within fern spores suggest that storage at subfreezing temperatures will give longevities comparable with those achieved with seeds. However, the window of optimum water contents for fern spores is very narrow and much lower than that measured in seeds, making precise manipulation of water content imperative for achieving maximum longevity.Key words: Ex situ conservation, germplasm, relative humidity, temperature, water sorption isotherms. IntroductionPteridophytes (ferns) are associated with ecosystems that are particularly sensitive to degradation, and some taxa are in peril and require strict protection. Ex situ conservation provides an important backup strategy, as pteridophytes are very sensitive to environmental perturbations. Germplasm banks provide ex situ conservation of many plants by preserving seeds for decades or centuries (Gómez-Campo, 2001;Smith et al., 2003;Guerrant et al., 2004). However, methodologies for long-term preservation of pteridophytes in germplasm banks are not well established. Maintaining pteridophyte spore viability, genetic integrity, and capacity for normal growth are key research objectives to enable their effective ex situ conservation (Page et al., 1992).Long-term viability of pteridophyte spores depends on spore type or taxonomic group (Lloyd and Klekowski, 1970). The storage behaviour of the two types of spores recognized-green and non-green-is very different. Green spores are chlorophyllous and usually lose viability within weeks (e.g. Equisetum sp.) or months (e.g. Osmunda regalis), although survival for almost a year has been reported for a few genera stored at room temperature (e.g. Onoclea and Matteuccia) (Lloyd and Klekowsk...

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Molecular mobility in the cytoplasm: An approach to describe and predict lifespan of dry germplasm

Cited by 84 publications

References 46 publications

Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

Short-Term Liquid Nitrogen Storage of Maize, Common Bean and Soybean Seeds Modiﬁes Their Biochemical Composition

Water properties in fern spores: sorption characteristics relating to water affinity, glassy states, and storage stability

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