Molecular monitoring of protected fungi: mycelium persistence in soil after timber harvest

Gordon, Matthew; Norman, Kelli Van

doi:10.1016/j.funeco.2014.01.006

Cited by 9 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In practice, this means that searching and monitoring rare ECM fungal species, for example, red‐listed species of conservational interest, by DNA barcoding of soil cores are impractical. An exception may be when soil sampling is used to monitor‐specific locations with known occurrences of rare ECM fungi (Gordon & van Norman, ). Otherwise, extensive sporocarp search during years with good fruiting is a better approach, as large areas can be completely and efficiently searched.…”

Section: Discussionmentioning

confidence: 99%

“…impractical. An exception may be when soil sampling is used to monitor-specific locations with known occurrences of rare ECM fungi (Gordon & van Norman, 2014). Otherwise, extensive sporocarp search during years with good fruiting is a better approach, as large areas can be completely and efficiently searched.…”

Section: Constrains With Dna-based Fungal Soil Studiesmentioning

confidence: 99%

See 1 more Smart Citation

The significance of retention trees for survival of ectomycorrhizal fungi in clear‐cut Scots pine forests

Sterkenburg

Clemmensen

Lindahl

et al. 2019

Journal of Applied Ecology

View full text Add to dashboard Cite

1. Forestry with short stand generations and simplified forest structures has markedly affected forest biodiversity. One group of organisms adversely affected by clear-cutting is ectomycorrhizal (ECM) fungi, as they are associated with the roots of living trees. Retention forestry is a way of reducing logging impacts and enhancing biodiversity conservation. Increasing the proportion of trees retained at harvest may improve ECM fungal diversity.2. We investigated the potential for lifeboating of ECM fungi through the harvesting phase in an experimental field study in a 190-year-old Scots pine forest in northern Sweden. The experiment comprised four levels of tree retention-unlogged forest, plots with 60% or 30% of evenly distributed trees retained and clear-cuts without retained trees. We sampled soils and determined identities, frequencies and relative abundances of ECM fungal species during 3 years following logging through the use of high-throughput sequencing of amplified ITS2 markers.3. We identified 149 ECM fungal species, with the five most abundant species accounting for 50% of the total ECM fungal amplicons. Three years after harvesting, the proportion of ECM sequences in the total amplicon pool had decreased proportionally to the extent of tree removal. In clear-cuts, ECM fungal relative abundance had decreased by 95%, while ECM fungal species richness had declined by 75%, compared to unlogged plots. 4. Tree retention enabled the maintenance of the most frequent ECM species, while more lowly abundant species were progressively lost at random with increasing level of tree removal. Five of the most frequent ECM fungal species remained present after clear-cutting, probably associated with pine seedlings. Synthesis and applications.Tree retention can moderate short-term and potentially also long-term logging impacts on ECM fungi. Local ECM fungal diversity is preserved in proportion to the amount of retained trees. Abundant species may be largely maintained, even by low levels of tree retention and on naturally established seedlings. However, conservation of more infrequent species requires higher levels of tree retention, and our results suggest that around 75% of the ECM species are lost with the forest certification standard of 5% retention trees left at logging. tension limit of the boreal biome, as we wanted to study a diverse ECM fungal community unaffected by modern forestry. Scots pine forest is the most abundant type of forest ecosystem in northern Fennoscandia and commonly monodominant with respect to tree species (Stokland et al., 2003). K E Y W O R D S biodiversity, conservation, ectomycorrhizal fungal community, forest management, highthroughput sequencing, logging, soil, tree retention

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Constrains With Dna-based Fungal Soil Studiesmentioning

confidence: 99%

The significance of retention trees for survival of ectomycorrhizal fungi in clear‐cut Scots pine forests

Sterkenburg

Clemmensen

Lindahl

et al. 2019

Journal of Applied Ecology

View full text Add to dashboard Cite

show abstract

“…Using molecular identification techniques it has been shown that stipitate hydnoid fungi can be present and active belowground and not fruit for several years (van der Linde et al, 2009(van der Linde et al, , 2012Gordon & Van Norman, 2014). This suggests that, for stipitate hydnoids, the sporadic fruiting patterns may be explained by variation in fruit body production between years, rather than rapid turnover of populations.…”

Section: Main Textmentioning

confidence: 99%

Rainfall and temperature effects on fruit body production by stipitate hydnoid fungi in Inverey Wood, Scotland

Jarvis

Taylor

2017

Fungal Ecology

View full text Add to dashboard Cite

The NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. © 2017 Elsevier Ltd and British Mycological SocietyThis manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This version available http://nora.nerc.ac.uk/516893/ NERC has developed NORA to enable users to access research outputs wholly or partially funded by NERC. Copyright and other rights for material on this site are retained by the rights owners. Users should read the terms and conditions of use of this material at http://nora.nerc.ac.uk/policies.html#access NOTICE: this is the author's version of a work that was accepted for publication in Fungal Ecology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Ecology (2017), 27 (A). 137-140. 10.1016/j.funeco.2017.03 Main textThe occurrence and abundance of fungal fruit bodies are variable under natural conditions. Where surveys have been conducted in the same area over multiple years, many species are not observed in all years (Sato et al., 2012) and there is often large variation in the number of fruit bodies produced when fruiting does occur (Straatsma et al., 2001; Pilz et al., 2006). It is important to understand the drivers of fruit body production because the presence or absence of fruit bodies is the primary measure used to determine the conservation status of fungi. Stipitate hydnoid fungi are ectomycorrhizal fungi which are considered to be rare in the United Kingdom based on infrequent observation of fruit bodies and evidence of declining occurrence across Europe (Newton et al., 2002). It has been noted that these fungi produce fruit bodies sporadically, with sometimes several years between fruit body records (van der Linde et al., 2012).Using molecular identification techniques it has been shown that stipitate hydnoid fungi can be present and active belowground and not fruit for several years (van der Linde et al., 2009Linde et al., , 2012 Gordon & Van Norman, 2014). This suggests that, for stipitate hydnoids, the sporadic fruiting patterns may be explained by variation in fruit body production between years, rather than rapid turnover of populations.One potential driver of interannual variation in fruit body production is weather. Both the timing and production of fruit bodies have been linked to yearly variation in temperature and rainfall (Krebs et al., 2008; Boddy et al., 2014). There is evidence from previous studies that lagged weather influences on fungal fruiting can be important, for example temperature in previous months or years (Krebs et al., 2008; Ágreda et al., 2016). To narrow down the large ...

show abstract

“…Primer design for this purpose is mainly based on ITS, tef1 and rpb2 sequences and up to 14 species could be distinguished by applying this approach [57]. A similar multiplex PCR system has also been developed for the genus Trichoderma, which is capable of differentiating four species of the genus in a single PCR reaction [58].…”

Section: Strain Discrimination Using Multiplex Pcrmentioning

confidence: 99%

SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains

et al. 2015

View full text Add to dashboard Cite

Using biological fertilizers and pesticides based on beneficial soil microbes in order to reduce mineral fertilizers and chemical pesticides in conventional agriculture is still a matter of debate. In this regard, a European research project seeks to elucidate the role of root-endophytic fungi and to develop molecular tools to trace and quantify these fungi in the rhizosphere and root tissue. To do this, the draft genome sequence of the biocontrol fungus Trichoderma virens (T. virens) was screened for simple sequence repeats (SSRs) and primers were developed for 12 distinct loci. Primers were evaluated using a global collection of ten isolates where an average of 7.42 alleles per locus was detected. Nei's standard genetic distance ranged from 0.18 to 0.27 among the isolates, and the grand mean of haploid diversity in AMOVA analysis was 0.693 ± 0.019. Roots of tomato plants were inoculated with different strains and harvested six weeks later. Subsequent PCR amplification identified root-endophytic strains and co-colonization of roots by different strains. Markers were applied to qPCR to quantify T. virens strains in root tissue and to determine their identity using allele-specific melting curve analysis. Thus, the root-endophytic lifestyle of T. virens was OPEN ACCESSDiversity 2015, 7 361 confirmed, strains in roots were quantified and simultaneous colonization of roots by different strains was observed.

show abstract

Molecular monitoring of protected fungi: mycelium persistence in soil after timber harvest

Cited by 9 publications

References 37 publications

The significance of retention trees for survival of ectomycorrhizal fungi in clear‐cut Scots pine forests

The significance of retention trees for survival of ectomycorrhizal fungi in clear‐cut Scots pine forests

Rainfall and temperature effects on fruit body production by stipitate hydnoid fungi in Inverey Wood, Scotland

SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains

Contact Info

Product

Resources

About