Molecular Motor-Powered Shuttles along Multi-walled Carbon Nanotube Tracks

Sikora, A.; Ramón‐Azcón, Javier; Kim, Kyongwan; Reaves, K.; Nakazawa, Hikaru; Umetsu, Mitsuo; Kumagai, Izumi; Adschiri, Tadafumi; Shiku, Hitoshi; Matsue, Tomokazu; Hwang, Wonmuk; Teizer, W.

doi:10.1021/nl4042388

Cited by 20 publications

(26 citation statements)

References 48 publications

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“…This new approach facilitates biomicro/nano devices requiring advanced capabilities, such as instantaneous transformation of the track configuration, three dimensional delivery, active aiming, and chip-to-chip communication. With wire diameters down to submicrometer range this method is complementary, in terms of the dimension and flexibility, to other methods using nanometer scale wire-type guiding templates for molecular shuttles (Sikora et al, 2014;Byun et al, 2009;ten Sietoff et al, 2013). The glass wire-based approach presented here is, in general, applicable to different molecular motility systems, e.g.…”

Section: Discussionmentioning

confidence: 99%

Microtubule shuttles on kinesin-coated glass micro-wire tracks

Kim

Liao

Sikora

et al. 2014

Biomed Microdevices

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Gliding of microtubule filaments on surfaces coated with the motor protein kinesin has potential applications for nano-scale devices. The ability to guide the gliding direction in three dimensions allows the fabrication of tracks of arbitrary geometry in space. Here, we achieve this by using kinesin-coated glass wires of micrometer diameter range. Unlike previous methods in which the guiding tracks are fixed on flat two-dimensional surfaces, the flexibility of glass wires in shape and size facilitates building in-vitro devices that have deformable tracks.

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Section: Discussionmentioning

confidence: 99%

Microtubule shuttles on kinesin-coated glass micro-wire tracks

Kim

Liao

Sikora

et al. 2014

Biomed Microdevices

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“…The average speeds measured on the myosin-coated CNT (8.7 ± 3.5 μm/s; n = 32) were comparable to those measured on the glass surface in control experiments (6.0 ± 1.2 μm/s; n = 20), indicating that myosin motors retain their normal motile activity when adsorbed onto a CNT. Earlier studies with CNTs or semiconductor nanowires had reported sliding speeds far below the physiological speed-less than 20 % of that of kinesin (Sikora et al 2014) and~42 % of that of myosin (Siethoff et al 2014). The normal level of motility shown by the CNT-adsorbed myosin molecule in this system (Inoue et al 2015) is the first demonstration that a single CNT can be used as a platform to evaluate this protein's normal function as a myosin motor.…”

Section: Observation Of Motor Function On a Single Cntmentioning

confidence: 82%

“…Tsuchiya et al (2010) constructed a container transport system, powered by myosin-VI that can move CNTs along an intracellular track of actin filaments. Multi-walled CNTs and semiconductor nanowires can be used as a onedimensional (1-D) track for the myosin-based sliding of actin filaments (Siethoff et al 2014) and the kinesin-based sliding of microtubules (Sikora et al 2014). Motor proteins are compatible with CNTs and produce activity that is easily observed, such as actin-binding kinetics and enzyme-based motile activity.…”

Section: Motor Proteinsmentioning

confidence: 99%

Local heating of molecular motors using single carbon nanotubes

Inoue

Ishijima

2016

Biophys Rev

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Temperature globally affects all chemical processes and biomolecules in living cells. Elevating the temperature of an entire cell accelerates so many biomolecular reactions simultaneously that it is difficult to distinguish the various mechanisms involved. The ability to localize temperature changes to the nanometer range within a cell could provide a powerful new tool for regulating biomolecular activity at the level of individual molecules. The search for a nanoheater for biological research has prompted experiments with carbon nanotubes (CNTs), which have the highest conductivity of any known material. The adsorption of skeletal muscle myosin molecules along the length of single multi-walled CNTs (~10 μm) has allowed researchers to observe the ATP-driven sliding of fluorescently labeled actin filaments. In one study, red-laser irradiation focused on one end of a myosin-coated CNT was used to heat myosin motors locally without directly heating the surrounding water; this laser irradiation instantly accelerated the actin-filament sliding speeds from~6 to~12 μm/s in a reversible manner, indicating a local, real-time heating of myosin motors by approximately Δ12 K. Calculation of heat transfer using the finite element method, based on the estimated temperature along a single CNT with a diameter of 170 nm, indicated a high thermal conductivity of~1540 Wmtion, consistent with values measured in vacuum in earlier studies. Temperature distribution indicated by half-decrease distances was~3660 nm along the length of the CNT and 250 nm perpendicular to the length. These results suggest that single-CNT-based heating at the nanometer-or micrometerrange could be used to regulate various biomolecules in many areas of biological, physical, and chemical research.

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“…The biotinylation treatment and the MWCNT functionalization followed the protocol described elsewhere (Sikora et al 2014). After surface modification, a 50 μm thick spacer was placed between the chip and a glass substrate to form a chamber for introduction of 0.4 mg/ml streptavidin modified MWCNTs.…”

Section: Hexagonal and Octagonal Device Fabricationmentioning

confidence: 99%

“…This technique is easy to apply and can be performed in water, making it compatible with protein functionalized MWCNTs. Recently, we have shown that it is possible to align and attach MWCNTs on a functionalized surface in order to build tracks allowing MT gliding (Sikora et al 2014). Here we show how the width of the MWCNT track can be modulated and how to limit the MWCNT dispersion.…”

Section: Introductionmentioning

confidence: 99%

Microtubule guiding in a multi-walled carbon nanotube circuit

Sikora

Ramón‐Azcón

Şen

et al. 2015

Biomed Microdevices

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In nanotechnological devices, mass transport can be initiated by pressure driven flow, diffusion or by employing molecular motors. As the scale decreases, molecular motors can be helpful as they are not limited by increased viscous resistance. Moreover, molecular motors can move against diffusion gradients and are naturally fitted for nanoscale transportation. Among motor proteins, kinesin has particular potential for lab-on-a-chip applications. It can be used for sorting, concentrating or as a mechanical sensor. When bound to a surface, kinesin motors propel microtubules in random directions, depending on their landing orientation. In order to circumvent this complication, the microtubule motion should be confined or guided. To this end, dielectrophoretically aligned multi-walled-carbon nanotubes (MWCNT) can be employed as nanotracks. In order to control more precisely the spatial repartition of the MWCNTs, a screening method has been implemented and tested. Polygonal patterns have been fabricated with the aim of studying the guiding and the microtubule displacement between MWCNT segments. Microtubules are observed to transfer between MWCNT segments, a prerequisite for the guiding of microtubules in MWCNT circuit-based biodevices. The effect of the MWCNT organization (crenellated or hexagonal) on the MT travel distance has been investigated as well.

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Molecular Motor-Powered Shuttles along Multi-walled Carbon Nanotube Tracks

Cited by 20 publications

References 48 publications

Microtubule shuttles on kinesin-coated glass micro-wire tracks

Microtubule shuttles on kinesin-coated glass micro-wire tracks

Local heating of molecular motors using single carbon nanotubes

Microtubule guiding in a multi-walled carbon nanotube circuit

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