Molecular Nature of Two Nonconjugative Plasmids Carrying Drug Resistance Genes

Guerry, Patricia; Embden, Jan Van; Falkow, Stanley

doi:10.1128/jb.117.2.619-630.1974

Cited by 226 publications

(67 citation statements)

References 39 publications

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“…To determine whether the destabilizing effect of DpiA overexpression is restricted to pSC101, we tested plasmids carrying other replicons. We found that the mini-F plasmid pMF21 (Kline and Trawick, 1983) and a R6K derivative (pRR15, Roberts et al, 1990) were also strongly destabilized by pHI1447; however, the stability of a P1 plasmid derivative (pALA110, Abeles et al, 1985), the broad host range plasmid RSF1010 (Guerry et al, 1974) and the ColD derivative, pGZ119 (Lessl et al, 1992), was not affected ( Table 1).…”

Section: Dpia Affects Plasmids Other Than Psc101mentioning

confidence: 91%

Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two‐component system regulator

Ingmer

Miller

Cohen

1998

Molecular Microbiology

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SummaryWe identified a gene (dpiA , destabilizer of plasmid inheritance) which, when overexpressed in Escherichia coli, destabilizes the inheritance of pSC101 and other iteron-containing plasmids as disparate as mini-F and RK6 but not the inheritance of P1, RSF1010 and ColD. These effects of DpiA, which functions like an effector protein for a previously undescribed two-component signal transduction system, were reduced by mutations known to promote pSC101 replication and partitioning. dpiB, a gene encoding the putative histidine kinase of this two-component system, is located immediately 5Ј to dpiA and adjacent to a DpiA-induced target promoter that transcribes genes having homology to citrate lyase operon genes, citC, citD and citE, of Klebsiella pneumoniae. Disruption of dpiB reversed or reduced the effect of DpiA overproduction on pSC101 inheritance. A second DpiA target, the promoter for a gene (appY ) implicated in E. coli's response to anaerobiosis, is repressed by DpiA. A mutation in dpiA at a site commonly conserved and phosphor ylated in two-component system effector proteins abolished the effects of DpiA overproduction on pSC101 inheritance and negative regulation of appY expression. Our findings suggest a possible mechanism by which environmental and/or cellular stimuli may influence plasmid inheritance.

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Section: Dpia Affects Plasmids Other Than Psc101mentioning

confidence: 91%

Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two‐component system regulator

Ingmer

Miller

Cohen

1998

Molecular Microbiology

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“…IncQ plasmids contain replicon and mobilization modules in the backbone (24). The RSF1010, R1162, and R300B plasmids isolated independently from Escherichia coli, Pseudomonas aeruginosa, and Salmonella enterica serovar Typhimurium, respectively, are the best-characterized members of this group (4, 25, 26). In each of these plasmids, the minimal replicon consists of repA, repB and repC genes, which encode helicase, primase, and iteron-binding proteins, respectively, and an oriV region containing the origin of replication (27).…”

Section: Introductionmentioning

confidence: 99%

Evolution of satellite plasmids can stabilize the maintenance of newly acquired accessory genes in bacteria

Zhang

Deatherage

Zheng

et al. 2019

Preprint

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12Plasmids play a principal role in the spread of antibiotic resistance and other traits by horizontal 13 gene transfer in bacteria. However, newly acquired plasmids generally impose a fitness burden 14 on a cell, and they are lost from a population rapidly if there is not selection to maintain a unique 15 function encoded on the plasmid. Mutations that ameliorate this fitness cost can sometimes 16 eventually stabilize a plasmid in a new host, but they typically do so by inactivating some of its 17 novel accessory genes. In this study, we identified an additional evolutionary pathway that can 18 prolong the maintenance of newly acquired genes encoded on a plasmid. We discovered that 19 propagation of an RSF1010-based IncQ plasmid in Escherichia coli often generated 'satellite 20 plasmids' with spontaneous deletions of accessory genes and genes required for plasmid 21 replication. These smaller plasmid variants are nonautonomous genetic parasites. Their presence 22 Significance Statement 30Plasmids are multicopy DNA elements found in bacteria that replicate independently of a cell's 31 chromosome. The spread of plasmids carrying antibiotic-resistance genes to new bacterial 32 pathogens is a challenge for treating life-threatening infections. Often plasmids or their accessory 33 genes encoding unique functions are lost soon after transfer into a new cell because they impose 34 a fitness burden. We report that a family of transmissible plasmids can rapidly evolve 'satellite 35 plasmids' that replicate as genetic parasites of the original plasmid. Satellite plasmid formation 36 reduces the burden from the newly acquired genes, which may enable them to survive intact for 37 longer after transfer into a new cell and thereby contribute to the spread of antibiotic resistance 38 and other traits within bacterial populations. 39Recently acquired plasmids generally impose a fitness burden on a new host cell, which may 53 be due to the cost of expressing antibiotic resistance genes (6) or due to plasmid-encoded genes 54 interfering with host processes (7-9). Therefore, purifying selection can rapidly lead to the loss 55 of genes encoded on a plasmid from a new host unless there is positive selection for traits they 56 confer (10). Continuous conjugative transfer to spread a plasmid to new cells within a population 57 can counteract the purifying selection process to some extent, but there is also a fitness cost of 58 increasing the conjugation rate on donor cells (11). Compensatory mutations on the chromosome 59 and/or plasmid can sometimes ameliorate the cost of a plasmid and favor its persistence (12-17). 60However, it takes a long time, typically several hundred cell generations, with constant selection 61 5 for novel plasmid function to evolve and fix these types of compensatory mutations in laboratory 62 experiments (12, 18, 19). These conditions may be unrealistic in most natural settings. 63Most experimental work examining BHR plasmid stability has used IncP plasmids as a 64 model (13, 14, 17). Fewer studies have focu...

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“…Most of the vectors for cloning into methylotrophic bacteria have been constructed on the basis of RSF 1010 and other plasmids of the Inc P4 incompatibility group [2, 3,9]. The small multicopy plasmid RSF 1010 [4] is stably maintained in a wide range of gram-negative bacteria [5, 61 and can efficiently be mobilized by various conjugative plasmids [7].…”

Section: Introductionmentioning

confidence: 99%

Construction of a promoter‐probe vector for the methanol‐utilizing bacterium acetobacter methanolicus MB 58

Schröder

Engel

Chistoserdov

et al. 1989

Acta Biotechnologica

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We report here the construction of a promoter-probe vector, pRS2, which can be utilized in either Acetobacter methanolicvs MB 58 or Escherichiu .wli due to the presence of broad-host-range replicon RSF 1010. The vector provides several unique restriction sites for promoter cloning as well as resistance markers for the selection of transformanta. The promoter-probe vector was constructed by inserting a n EceoRI-13alI-polylinker fragment-of pUC 19 into EeoRI/SalI digested pMK 16. The resulting plasmid, pRS1, was cloned into the unique EcoRI site of the ,broad-host-range plasmid RSF 1010. The vector was used to clone promoter-containing sequences derived from the A. methanolicus MB 58 chromosome as well as the E. wli lac-promoter.

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Molecular Nature of Two Nonconjugative Plasmids Carrying Drug Resistance Genes

Cited by 226 publications

References 39 publications

Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two‐component system regulator

Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two‐component system regulator

Evolution of satellite plasmids can stabilize the maintenance of newly acquired accessory genes in bacteria

Construction of a promoter‐probe vector for the methanol‐utilizing bacterium acetobacter methanolicus MB 58

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