Molecular Neuroimaging: The Basics

Roux, Lucia Le; Schellingerhout, Dawid

doi:10.1053/j.ro.2013.12.001

Cited by 6 publications

(4 citation statements)

References 44 publications

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“…This benefited their performances and extended the possible in vivo studies. Review articles compared these technologies 1, [11][12][13][14] and some noninvasive imaging methods 15,16 . Among the fluidic sampling probes, the microdialysis probe is a gold standard.…”

Section: Introductionmentioning

confidence: 99%

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On demand nanoliter sampling probe for collection of brain fluid

Teixidor

Novello

Ortiz

et al. 2022

Preprint

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Continuous fluidic sampling systems allow collection of brain biomarkers in vivo. Here, we propose a new sampling paradigm, Droplet on Demand (DoD), implemented in a microfabricated neural probe. It allows sampling droplets loaded with molecules from the brain extracellular fluid punctually, without the long transient equilibration periods typical of continuous methods. It uses an accurate fluidic sequence and correct operation is verified by the embedded electrodes. As a proof of concept, we demonstrated the application of this novel approach in vitro and in vivo, to collect glucose in the brain of mice, with a temporal resolution of 1-2 minutes and without transient regime. Absolute quantification of the glucose level in the samples was performed by direct infusion nanoelectrospray ionization Fourier transform mass spectrometry (nanoESI-FTMS). By adjusting the diffusion time and the perfusion volume of DoD, the fraction of molecules recovered in the samples can be tuned to mirror the tissue concentration at accurate points in time. This makes quantification of biomarkers in the brain possible within acute experiments of only 20 to 120 minutes. DoD provides a complementary tool to continuous microdialysis and push-pull sampling probes. The advances allowed by DoD will benefit quantitative molecular studies in the brain, namely for molecules involved in volume transmission or for protein aggregates that form in neurodegenerative diseases over long periods.

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Section: Introductionmentioning

confidence: 99%

“…This benefited their performances and extended the possible in vivo studies. Review articles compared these technologies 1,11–14 and some non-invasive imaging methods 15,16 .…”

Section: Introductionmentioning

confidence: 99%

On demand nanoliter sampling probe for collection of brain fluid

Teixidor

Novello

Ortiz

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Review articles compared these technologies 1,6,13−15 and some noninvasive imaging methods. 16,17 Among the fluidic sampling probes, the microdialysis probe is a gold standard. It possesses two capillaries surrounded by a semipermeable membrane at the tip, which confines the flow of perfusion fluid, called perfusate, within the probe on one side and contacts the tissue on the other side.…”

mentioning

confidence: 99%

“…This benefited their performances and extended the possible in vivo studies. Review articles compared these technologies ,,− and some noninvasive imaging methods. , …”

mentioning

confidence: 99%

On-Demand Nanoliter Sampling Probe for the Collection of Brain Fluid

Teixidor

Novello

Ortiz³

et al. 2022

Anal. Chem.

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Continuous fluidic sampling systems allow collection of brain biomarkers in vivo. Here, we propose a new sequential and intermittent sampling paradigm using droplets, called Droplet on Demand (DoD). It is implemented in a microfabricated neural probe and alternates phases of analyte removal from the tissue and phases of equilibration of the concentration in the tissue. It allows sampling droplets loaded with molecules from the brain extracellular fluid punctually, without the long transient equilibration periods typical of continuous methods. It uses an accurately defined fluidic sequence with controlled timings, volumes, and flow rates, and correct operation is verified by the embedded electrodes and a flow sensor. As a proof of concept, we demonstrated the application of this novel approach in vitro and in vivo, to collect glucose in the brain of mice, with a temporal resolution of 1–2 min and without transient regime. Absolute quantification of the glucose level in the samples was performed by direct infusion nanoelectrospray ionization Fourier transform mass spectrometry (nanoESI-FTMS). By adjusting the diffusion time and the perfusion volume of DoD, the fraction of molecules recovered in the samples can be tuned to mirror the tissue concentration at accurate points in time. Moreover, this makes quantification of biomarkers in the brain possible within acute experiments of only 20–120 min. DoD provides a complementary tool to continuous microdialysis and push–pull sampling probes. Thus, the advances allowed by DoD will benefit quantitative molecular studies in the brain, i.e., for molecules involved in volume transmission or for protein aggregates that form in neurodegenerative diseases over long periods.

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Development of Neuroimaging-Based Biomarkers in Psychiatry

Aydın

Arslan

2019

Advances in Experimental Medicine and Biology

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Molecular Neuroimaging: The Basics

Cited by 6 publications

References 44 publications

On demand nanoliter sampling probe for collection of brain fluid

On demand nanoliter sampling probe for collection of brain fluid

On-Demand Nanoliter Sampling Probe for the Collection of Brain Fluid

Development of Neuroimaging-Based Biomarkers in Psychiatry

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