Molecular organization in protein-lipid film on the water surface studied by x-ray standing wave measurements under total external reflection

Zheludeva, S. I.; Новикова, Н. Н.; Stepina, N. D.; Yurieva, E. A.; Konovalov, Oleg

doi:10.1016/j.sab.2008.10.039

Cited by 13 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even models of biological surfaces have been labeled, in one way or another, with heavy elements that are not naturally abundant in biological matter (7). Light chemical elements have so far only investigated in a grazing-incidence configuration with low spatial resolution (15,16).In the present work, we demonstrate that light, biologically relevant chemical elements such as P and S can be localized also in Bragg reflection configuration and thus with atom-scale resolution. This approach enables the label-free, high-resolution, element-specific structural investigation of biomolecular layers.…”

supporting

confidence: 56%

“…The angle-dependent fluorescence intensity thus contains information on the interfacial element distribution (7). This principle has been exploited for the study of interfacial phenomena involving solid, liquid, and gas phases (8)(9)(10)(11)(12)(13)(14)(15)(16). High resolution perpendicular to the interface is implied by SWXF experiments in Bragg reflection configuration, in which case planar, nanometric multilayers are used to create strongly modulated standing waves above the terminal surface close to the Bragg condition (9,10).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Atom-scale depth localization of biologically important chemical elements in molecular layers

Schneck

Scoppola

Drnec

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

In nature, biomolecules are often organized as functional thin layers in interfacial architectures, the most prominent examples being biological membranes. Biomolecular layers play also important roles in context with biotechnological surfaces, for instance, when they are the result of adsorption processes. For the understanding of many biological or biotechnologically relevant phenomena, detailed structural insight into the involved biomolecular layers is required. Here, we use standing-wave X-ray fluorescence (SWXF) to localize chemical elements in solid-supported lipid and protein layers with near-Ångstrom precision. The technique complements traditional specular reflectometry experiments that merely yield the layers' global density profiles. While earlier work mostly focused on relatively heavy elements, typically metal ions, we show that it is also possible to determine the position of the comparatively light elements S and P, which are found in the most abundant classes of biomolecules and are therefore particularly important. With that, we overcome the need of artificial heavy atom labels, the main obstacle to a broader application of high-resolution SWXF in the fields of biology and soft matter. This work may thus constitute the basis for the label-free, element-specific structural investigation of complex biomolecular layers and biological surfaces.surfaces | interfaces | lipid membranes | protein adsorption | X-ray scattering N anometric biomolecular layers in interfacial geometries are key components of biological matter. Biological membranes, for example, are 2D molecular structures composed mainly of lipids and proteins in an aqueous environment (1, 2). Interfacial biomolecular layers are also important from a biotechnological perspective, where surfaces are often designed to selectively promote or prevent the adsorption of bio(macro)molecules from solution (3). To investigate the diverse behavior of biomolecules at interfaces, well-defined planar model systems of biological and biotechnologically relevant interfaces have been established (4, 5). Processes involving biomolecules at interfaces are typically accompanied by a spatial reorganization of molecules, changes in molecular conformations, or the adsorption of molecules in a chemically heterogeneous environment (6). Structural insight on the nanometer scale is therefore of paramount importance.X-ray and neutron scattering are uniquely suited for the structural investigation of biomolecular systems. Specular reflectometry reveals matter density profiles perpendicular to a planar interface. However, it is generally difficult to elucidate molecular conformations and elemental distributions from such "global" density profiles. In contrast, standing-wave X-ray fluorescence (SWXF) allows determining element-specific density profiles across an interface. The SWXF technique is based on the elementcharacteristic fluorescence induced by a standing X-ray wave. The latter is formed by interference of the incident and reflected waves and its shape depends o...

show abstract

supporting

confidence: 56%

mentioning

confidence: 99%

See 1 more Smart Citation

Atom-scale depth localization of biologically important chemical elements in molecular layers

Schneck

Scoppola

Drnec

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Adapted from [30]. a handful of studies report on the localization of such light elements by XSW fluorescence [62,66,67]. For example, Zheludeva et al determined the distribution of phosphorus in a relatively thick (N100 nm) protein/ phospholipid film at an air/water interface [67].…”

Section: Light Elements At Soft Interfacesmentioning

confidence: 99%

“…a handful of studies report on the localization of such light elements by XSW fluorescence [62,66,67]. For example, Zheludeva et al determined the distribution of phosphorus in a relatively thick (N100 nm) protein/ phospholipid film at an air/water interface [67]. All these studies dealt with either gas/liquid or gas/solid interfaces, where limitations due to the adsorption of low-energy incident and fluorescence radiation as well as undesired background fluorescence are largely circumvented.…”

Section: Light Elements At Soft Interfacesmentioning

confidence: 99%

Structural characterization of soft interfaces by standing-wave fluorescence with X-rays and neutrons

Schneck

Demé

2015

Current Opinion in Colloid & Interface Science

View full text Add to dashboard Cite

We present a review of standing-wave fluorescence techniques with x-rays and neutrons for the elementspecific structural investigation of interfaces. The basic principles are introduced and typical measurement configurations with their advantages and limitations are compared. An overview of studies dealing with various types of interfaces is given. In particular, work on soft and biological matter in planar, interfacial geometries is discussed.

show abstract

Mixed alkaline phosphatase/sphingomyelin monolayer at the air-buffer interface: phase behavior and morphology

2014

View full text Add to dashboard Cite

Molecular organization in protein-lipid film on the water surface studied by x-ray standing wave measurements under total external reflection

Cited by 13 publications

References 12 publications

Atom-scale depth localization of biologically important chemical elements in molecular layers

Atom-scale depth localization of biologically important chemical elements in molecular layers

Structural characterization of soft interfaces by standing-wave fluorescence with X-rays and neutrons

Mixed alkaline phosphatase/sphingomyelin monolayer at the air-buffer interface: phase behavior and morphology

Contact Info

Product

Resources

About