2014
DOI: 10.1002/chem.201403897
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Molecular Origin of the Hydrolytic Activity and Fixed Regioselectivity of a ZrIV‐Substituted Polyoxotungstate as Artificial Protease

Abstract: A multitechnique approach has been applied in order to identify the thermodynamic and kinetic parameters related to the regioselective hydrolysis of human serum albumin (HSA) or Cys392ÀGlu393 peptide bonds. This is in agreement with the interaction studies as the Arg114ÀLeu115 peptide bond is located in the positive cleft of HSA and the three remaining peptide bonds are each located near an upstream acidic residue, which can be expected to coordinate to the metal ion. A detailed kinetic study has evidenced t… Show more

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Cited by 4 publications
(8 citation statements)
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“…Zr(IV)‐substituted polyoxometalates (Zr‐POMs, I P in Figure ) are metal‐oxygen clusters that do not exhibit substrate specificity and hydrolyze multiple peptide bonds of many critical molecules such as human serum albumin (HSA), HEWL, myoglobin, insulin chain B and cytochrome c . Among these substrates, HSA has been considered as an excellent model to study the physicochemical basis of drug–protein interactions due to its biological functions (Figure ) .…”
Section: Peptide and Phosphoester Hydrolysis By Synthetic Analoguesmentioning
confidence: 99%
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“…Zr(IV)‐substituted polyoxometalates (Zr‐POMs, I P in Figure ) are metal‐oxygen clusters that do not exhibit substrate specificity and hydrolyze multiple peptide bonds of many critical molecules such as human serum albumin (HSA), HEWL, myoglobin, insulin chain B and cytochrome c . Among these substrates, HSA has been considered as an excellent model to study the physicochemical basis of drug–protein interactions due to its biological functions (Figure ) .…”
Section: Peptide and Phosphoester Hydrolysis By Synthetic Analoguesmentioning
confidence: 99%
“…Recently, I P complexes have been proposed as novel artificial metalloproteases and nucleases. [64][65][66][67][68][69][70][71] They are oxo-anion bridged clusters of metal ions such as Ti(IV), Zr(IV), and Ce(IV) that have been reported to hydrolyze hen egg white lysozyme (HEWL), albumins, myoglobin, DNA, and RNA model substrates. [64][65][66][67][72][73][74] Although I C complex is much smaller and simpler than I P it can also degrade albumin, lysozyme, Aβ peptide, β-lactam, and DNA.…”
Section: Peptide and Phosphoester Hydrolysismentioning
confidence: 99%
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“…3 Among them, the group of Parac-Vogt probed the hydrolytic activity of Zr(IV)-, Ce(IV) and Hf(IV)-substituted polyoxometalates (POMs) towards dipeptides and oligopeptides, 4,5 and more importantly, towards peptide bonds in proteins in a highly selective manner. [6][7][8][9][10][11][12] Zr-substituted POMs (see some structures in Figure 1A) were found to hydrolyze horse heart myoglobin (HHM) 9 and hemoglobin (Hb) 12 only at peptide bonds of Asp-X topology, where X is any other amino acid. In contrast, other proteins such as human serum albumin (HSA) 7 or hen egg-white lysozyme (HEWL) 6 were found to be selectively hydrolyzed at chemically different sites.…”
Section: Introductionmentioning
confidence: 99%