Molecular Pathology of Chronic Lymphocytic Leukemia

Hoehn, Daniela; Medeiros, L. Jeffrey; Konoplev, Sergej

doi:10.1007/978-1-60761-262-9_8

Cited by 2 publications

(7 citation statements)

References 193 publications

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Section: Introductionmentioning

confidence: 99%

“…Chronic lymphocytic leukemia (CLL) is the most commonly observed hematological malignancy in Western countries ( 1 – 3 ). It represents 0.8% of all the neoplasms and almost 30% of all the leukemia cases documented worldwide ( 1 – 3 ). CLL affects particularly the elderly, since the median age at diagnosis is 72 years, although increased morbidity among younger patients has been recently reported ( 1 – 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…It represents 0.8% of all the neoplasms and almost 30% of all the leukemia cases documented worldwide ( 1 – 3 ). CLL affects particularly the elderly, since the median age at diagnosis is 72 years, although increased morbidity among younger patients has been recently reported ( 1 – 3 ). The incidence of CLL is 2–6 cases/100,000 patients/year, and it increases with age ( 1 – 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…CLL affects particularly the elderly, since the median age at diagnosis is 72 years, although increased morbidity among younger patients has been recently reported ( 1 – 3 ). The incidence of CLL is 2–6 cases/100,000 patients/year, and it increases with age ( 1 – 3 ). The risk of malignancy development is double in men compared with women ( 1 – 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…The incidence of CLL is 2–6 cases/100,000 patients/year, and it increases with age ( 1 – 3 ). The risk of malignancy development is double in men compared with women ( 1 – 3 ). CLL is a lymphoproliferative disorder that may be characterized by the accumulation of small, homogeneous, mature cluster of differentiation (CD)5 + CD19 + B-lymphocytes in the peripheral blood, bone marrow and secondary lymphoid organs ( 3 , 4 ).…”

Section: Introductionmentioning

confidence: 99%

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Laccase purified from Cerrena unicolor exerts antitumor activity against leukemic cells

et al. 2016

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Chronic lymphocytic leukemia (CLL) is the most commonly observed adult hematological malignancy in Western countries. Despite the fact that recent improvements in CLL treatment have led to an increased percentage of complete remissions, CLL remains an incurable disease. Cerrena unicolor is a novel fungal source of highly active extracellular laccase (ex-LAC) that is currently used in industry. However, to the best of our knowledge, no reports regarding its anti-leukemic activity have been published thus far. In the present study, it was hypothesized that C. unicolor ex-LAC may possess cytotoxic activity against leukemic cell lines and CLL primary cells. C. unicolor ex-LAC was separated using anion exchange chromatography on diethylaminoethyl cellulose-Sepharose and Sephadex G-50 columns. The cytotoxic effects of ex-LAC upon 24- and 48-h treatment on HL-60, Jurkat, RPMI 8226 and K562 cell lines, as well as CLL primary cells of nine patients with CLL, were evaluated using 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. Annexin V/propidium iodide staining of Jurkat cells treated with ex-LAC was used to investigate apoptosis via flow cytometry. Ex-LAC induced changes in Jurkat and RPMI 8226 cells, as visualized by fluorescence and scanning electron microscopy (SEM). The XTT assay revealed high cytotoxic rates following treatment with various concentrations of ex-LAC on all the cell lines and CLL primary cells analyzed, with a half maximal inhibitory concentration ranging from 0.4 to 1.1 µg/ml. Fluorescence microscopy and SEM observations additionally revealed apoptotic changes in Jurkat and RPMI 8226 cells treated with ex-LAC, compared with control cells. These results were in agreement with the apoptosis analysis of Jurkat cells on flow cytometry. In conclusion, C. unicolor ex-LAC was able to significantly induce cell apoptosis, and may represent a novel therapeutic agent for the treatment of various hematological neoplasms.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Laccase purified from Cerrena unicolor exerts antitumor activity against leukemic cells

et al. 2016

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Surface-Enhanced Raman Scattering Dye-Labeled Au Nanoparticles for Triplexed Detection of Leukemia and Lymphoma Cells and SERS Flow Cytometry

et al. 2013

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The labeling of cell surface receptors by fluorescent markers is an established method for the identification of cell phenotype in both research and clinical settings. Fluorescence dye labeling has inherent constraints, most notably the upper limit of labels per cell that may be probed using a single excitation source, in addition to a physical limit to the number of broad emission spectra that can be distinctly collected within the visible wavelength region. SERS labeling has the potential to mitigate these shortfalls. Herein, antibody-targeted, PEG-coated surface-enhanced Raman scattering (SERS) Au nanoparticles are used simultaneously to label three cell surface markers of interest on malignant B cells from the LY10 lymphoma cell line. The SERS probes were characterized by multiple methods to confirm their monodispersity and functionalization with both PEG and monoclonal antibodies. The specificity of the particles' cell labeling was demonstrated on both primary chronic lymphocytic leukemia and LY10 cells using SERS from cell suspensions and confocal Raman mapping, respectively. Fluorescence flow cytometry was employed to confirm the binding of SERS probes to LY10 over large cell populations, and the particles' SERS was collected directly from labeled cells using a commercial flow cytometer. To the best of our knowledge, this is the first demonstration of SERS flow cytometry from cells tagged with targeted SERS probes.

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Molecular Pathology of Chronic Lymphocytic Leukemia

Abstract: Keywords Chronic lymphocytic leukemia (CLL) · Small lymphocytic lymphoma (SLL) · Genetic predisposition · Conventional cytogenetics · Fluorescence in situ hybridization (

Cited by 2 publications

References 193 publications

Laccase purified from Cerrena unicolor exerts antitumor activity against leukemic cells

Laccase purified from Cerrena unicolor exerts antitumor activity against leukemic cells

Surface-Enhanced Raman Scattering Dye-Labeled Au Nanoparticles for Triplexed Detection of Leukemia and Lymphoma Cells and SERS Flow Cytometry

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