Molecular Pathology of Endometrial Carcinoma on LBC Samples and Cell Blocks

Martins, Diana; Schmitt, Fernando; Frattini, Milo; Fulciniti, Franco

doi:10.1007/978-981-16-5011-6_16

Cited by 2 publications

(2 citation statements)

References 38 publications

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“…This is because formalin fixation, especially if protracted for more than 3 h, may be detrimental for molecular studies and, hence the study of cytologic rather than histologic samples could be advantageous in these patients, even over histology. 70,71 In cases with generous cell content and a positive cytologic diagnosis of carcinoma, an ideal sample workout could include LBC and cytomorphology, splitting the residual material in two aliquots, one for molecular pathology and the other one for cell block, histology and ICC.…”

Section: Molecular Classification Of Endometrial Carcinomamentioning

confidence: 99%

“…It is our personal opinion that, at least in cases with generous cell‐tissue representation in LBC samples from endometrial scrapings, molecular pathology should be used on the residual fluid. This is because formalin fixation, especially if protracted for more than 3 h, may be detrimental for molecular studies and, hence the study of cytologic rather than histologic samples could be advantageous in these patients, even over histology 70,71 …”

Section: Role Of Ancillary Techniques and Molecular Pathology In Mate...mentioning

confidence: 99%

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A review of the directly sampled endometrial cytology on LBC samples: Classification, microscopic criteria and beyond

Norimatsu,

Maeda,

Malara

et al. 2023

Cytopathology

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The Yokohama System for Reporting Endometrial Cytology (TYS) has been proposed by an expert meeting under the auspices of the International Academy of Cytology (IAC) in May 2016 at the IAC in Yokohama. Since its introduction, the TYS has been receiving worldwide acceptance, and this review aims to assess its global impact. The adoption of endometrial cytology as a diagnostic procedure has been hampered in the past by difficulties arising in interpreting the cellular findings due to a number of factors (such as excess blood, cellular overlapping and the complex physiology of endometrium). Recently, the use of liquid‐based cytology (LBC), with its ability to remove blood and mucus and to distribute cells uniformly in a thin layer on the slide, has provided an opportunity to re‐evaluate the role of endometrial cytology. LBC is a useful tool in the cytologic diagnosis and follow‐up of endometrial abnormalities, which remains complementary to the emerging molecular diagnostic cytopathology. The study of LBC from endometrial cytology could be challenging since it is affected by numerous look‐alikes and diagnostic pitfalls. This review discusses these various entities and takes into consideration the ancillary techniques that may be useful in the diagnostic procedure. In conclusion, our review of the published data suggests that the TYS is a valid classification scheme that has been widely accepted by cytopathologists globally, is highly reproducible and makes a valuable contribution to clinical therapeutic management. At present, molecular cytopathology is a rapidly evolving field of modern cytopathology, which underlines the effective interplay between genomics and cytology. This review aims to provide a comprehensive review of the drawbacks of endometrial cytopathology, particularly in terms of endometrial cancer diagnosis and molecular testing.

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Section: Molecular Classification Of Endometrial Carcinomamentioning

confidence: 99%

Section: Role Of Ancillary Techniques and Molecular Pathology In Mate...mentioning

confidence: 99%

A review of the directly sampled endometrial cytology on LBC samples: Classification, microscopic criteria and beyond

Norimatsu,

Maeda,

Malara

et al. 2023

Cytopathology

Self Cite

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Detection of P16/Ki-67 Markers in the Detection of Cervical Intraepithelial Neoplasia

Novokhatska,

Kalmukova,

Skrypnyk

et al. 2023

BTSNUKSB

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B a c k g r o u n d . In recent years, Ukraine has experienced an unfavorable epidemiological situation concerning sexually transmitted diseases. One rapidly spreading infection is genital human papillomavirus (HPV). Convincing research has identified human papillomavirus (HPV) as a significant factor in the development of malignant transformations in the cervix. The study of the molecular pathogenesis associated with HPV-related neoplasms has led to the discovery of biomarkers aiding in the diagnosis of High-grade Squamous Intraepithelial Lesion (HSIL) of the cervix. Including the biomarkers p16/Ki-67 in the investigation enhances sensitivity in detecting HSIL in women. Additionally, it is pertinent to further analyze the effectiveness of using an additional semi-quantitative scale to assess the results of immunocytochemical detection of p16/Ki-67. M e t h o d s . The research was conducted on biological material from cervical smears using liquid-based cytology. Techniques included Papanicolaou staining, HPV detection, and immunocytochemical staining against p16/Ki-67. The sample comprised 90 women divided into three age groups, each categorized based on criteria such as HPV presence/absence, Bethesda classification of dysplasia, and negative/positive p16/Ki-67 results. Intensity of staining was further scored on a scale in the corresponding location of positive reactions in the nucleus or cytoplasm. R e s u l t s . The study revealed that immunocytochemical markers p16 and Ki-67 identified women with intraepithelial lesions, especially in cases with positive HPV results and no dysplastic changes in the Pap test. The intensity of immunocytochemical staining for p16 and Ki-67 varied directly with the detected degree of dysplasia. C o n c l u s i o n s . A direct correlation was observed between the severity of dysplasia established by Bethesda classification and the intensity of immunocytochemical staining for p16 and Ki-67. These findings provide an opportunity for further exploration of staining intensity in the presence of immunocytochemical markers p16 and Ki-67 for their potential use in the comprehensive interpretation of Pap test results and the precise identification of dysplasia in ambiguous diagnostic samples.

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Molecular Pathology of Endometrial Carcinoma on LBC Samples and Cell Blocks

Cited by 2 publications

References 38 publications

A review of the directly sampled endometrial cytology on LBC samples: Classification, microscopic criteria and beyond

A review of the directly sampled endometrial cytology on LBC samples: Classification, microscopic criteria and beyond

Detection of P16/Ki-67 Markers in the Detection of Cervical Intraepithelial Neoplasia

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