Molecular, pharmacological, and signaling properties of octopamine receptors from honeybee (<i>Apis mellifera</i>) brain

Balfanz, Sabine; Jordan, Nadine; Langenstück, Teresa; Breuer, Johanna; Bergmeier, Vera; Baumann, Arnd

doi:10.1111/jnc.12619

Cited by 63 publications

(106 citation statements)

References 64 publications

(156 reference statements)

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“…The OAMB receptor is coupled to Ca 2+ accumulation (Zeng et al, 1996;Han et al, 1998;Grohmann et al, 2003;Balfanz et al, 2005;Evans and Maqueira, 2005). Orthologs of OAMB have subsequently been identified in Apis (Blenau and Baumann, 2001;Grohmann et al, 2003;Balfanz et al, 2014), Anopheles (Kastner et al, 2014), Schistocerca (Verlinden et al, 2010b), and Locusta (Hiripi et al, 1994;Ma et al, 2015), among other invertebrate species. Whether the OAMB receptor is a true OCT receptor or a mixed OCT/TYR receptor has been a matter of some recent conjecture (El-Kholy et al, 2015).…”

Section: Octopamine Receptorsmentioning

confidence: 99%

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Trace Amines and Their Receptors

Gainetdinov¹,

Hoener²,

Berry³

2018

Pharmacol Rev

297

287

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Section: Octopamine Receptorsmentioning

confidence: 99%

“…Like their vertebrate counterparts, all three b-receptors are coupled to the cAMP signal transduction cascade through activation of adenylyl cyclase (Chang et al, 2000;Maqueira et al, 2005;Balfanz et al, 2014). A fourth b-like receptor has also been suggested to be present in honeybees (Hauser et al, 2006;Balfanz et al, 2014).…”

Section: Octopamine Receptorsmentioning

confidence: 99%

Trace Amines and Their Receptors

Gainetdinov¹,

Hoener²,

Berry³

2018

Pharmacol Rev

297

287

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“…We suggest that the behavioral effects of tyramine strongly depend on the activation of specific tyramine receptors, which may act in a similar way to octopamine receptors but which may also have opposite effects, depending on the signaling cascades involved and on the specific tissues involved in the control of a certain behavior. The behavioral changes of tyramine that are similar to those of octopamine might be achieved through the second honeybee tyramine receptor, AmTYR2, which acts similarly to the octopamine receptors AmOCTβR1 to AMOCTβR4 (Balfanz et al, 2014;Reim et al, 2017) by upregulating cAMP. Octopamine similarly improves appetitive learning performance in honeybees, as does tyramine (Behrends and Scheiner, 2012).…”

Section: Function Of Tyramine Receptors In Gustatory Responsiveness Amentioning

confidence: 99%

“…Yohimbine is an effective antagonist of honeybee tyramine receptors (Reim et al, 2017) but does not block honeybee octopamine receptors (Balfanz et al, 2014). To test whether the tyramine-induced effect on gustatory responsiveness in nurse bees could be attributed to activation of tyramine receptors, we also tested effects of yohimbine on gustatory responsiveness.…”

Section: Tyramine Can Increase Gustatory Responsiveness and Improve Lmentioning

confidence: 99%

“…One of these tyramine receptors (AmTYR1) decreases intracellular cAMP concentrations ([cAMP] i ) when activated (Beggs et al, 2011;Blenau et al, 2000;Mustard et al, 2005), while the second tyramine receptor (AmTYR2) increases [cAMP] i after activation (Reim et al, 2017). Interestingly, this receptor acts similarly to four of the five honeybee octopamine receptors (Balfanz et al, 2014), which all increase [cAMP] i after activation. The differential effects on [cAMP] i of the two tyramine receptors and most of the honeybee octopamine receptors might explain why in some situations, octopamine acts similarly to tyramine, such as in assays involving gustatory responsiveness, in which octopamine and tyramine both increased responsiveness (Scheiner et al, 2002) or aversive learning, in which both octopamine and tyramine can reduce learning performance, although the tyramine effect was very weak (Agarwal et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Learning, gustatory responsiveness and tyramine differences across nurse and forager honeybees

Scheiner

Reim

Søvik

et al. 2017

Journal of Experimental Biology

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Honeybees are well known for their complex division of labor. Each bee sequentially performs a series of social tasks during its life. The changes in social task performance are linked to gross differences in behavior and physiology. We tested whether honeybees performing different social tasks (nursing versus foraging) would differ in their gustatory responsiveness and associative learning behavior in addition to their daily tasks in the colony. Further, we investigated the role of the biogenic amine tyramine and its receptors in the behavior of nurse bees and foragers. Tyramine is an important insect neurotransmitter, which has long been neglected in behavioral studies as it was believed to only act as the metabolic precursor of the better-known amine octopamine. With the increasing number of characterized tyramine receptors in diverse insects, we need to understand the functions of tyramine on its own account. Our findings suggest an important role for tyramine and its two receptors in regulating honeybee gustatory responsiveness, social organization and learning behavior. Foragers, which were more responsive to gustatory stimuli than nurse bees and performed better in appetitive learning, also differed from nurse bees in their tyramine brain titers and in the mRNA expression of a tyramine receptor in the brain. Pharmacological activation of tyramine receptors increased gustatory responsiveness of nurse bees and foragers and improved appetitive learning in nurse bees. These data suggest that a large part of the behavioral differences between honeybees may be directly linked to tyramine signaling in the brain.

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Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella

Huang

Deng

et al. 2018

Arch Insect Biochem Physiol

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The β-adrenergic-like octopamine receptor (OA2B2) belongs to the class of G-protein coupled receptors. It regulates important physiological functions in insects, thus is potentially a good target for insecticides. In this study, the putative open reading frame sequence of the Pxoa2b2 gene in Plutella xylostella was cloned. Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. PxOA2B2 was transiently expressed in HEK-293 cells. It was found that PxOA2B2 could be activated by both octopamine and tyramine, resulting in increased intracellular cyclic AMP (cAMP) levels, whereas dopamine and serotonin were not effective in eliciting cAMP production. Further studies with series of PxOA2B2 agonists and antagonists showed that all four tested agonists (e.g., naphazoline, clonidine, 2-phenylethylamine, and amitraz) could activate the PxOA2B2 receptor, and two of tested antagonists (e.g., phentolamine and mianserin) had significant antagonistic effects. However, antagonist of yohimbine had no effects. Quantitative real-time polymerase chain reaction analysis showed that Pxoa2b2 gene was expressed in all developmental stages of P. xylostella and that the highest expression occurred in male adults. Further analysis with fourth-instar P. xylostella larvae showed that the Pxoa2b2 gene was mainly expressed in Malpighian tubule, epidermal, and head tissues. This study provides both a pharmacological characterization and the gene expression patterns of the OA2B2 in P. xylostella, facilitating further research for insecticides using PxOA2B2 as a target.

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Molecular, pharmacological, and signaling properties of octopamine receptors from honeybee (Apis mellifera) brain

Cited by 63 publications

References 64 publications

Trace Amines and Their Receptors

Trace Amines and Their Receptors

Learning, gustatory responsiveness and tyramine differences across nurse and forager honeybees

Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella

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