Molecular phenotype of zebrafish ovarian follicle by serial analysis of gene expression and proteomic profiling, and comparison with the transcriptomes of other animals

Knoll‐Gellida, Anja; André, Michèle; Gattegno, Tamar; Forgue, Jean; Admon, Arie; Babin, Patrick J.

doi:10.1186/1471-2164-7-46

Cited by 84 publications

(61 citation statements)

References 93 publications

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“…Similarly, a large-scale gene transcription analysis by means of serial analysis of gene expression (SAGE) revealed high levels of ATP synthase transcripts in zebrafish ovarian fully-grown follicules. Complementary, the proteomic analysis carried out on the same tissue revealed high levels of the corresponding proteins, thus supporting the fact that ATP synthase mRNAs are effectively translated into proteins (Knoll-Gellida et al 2006). Concerning the Cd effect, whereas our previous results have shown a stimulating effect of Cd pre-exposure on the pituitary-gonad-liver axis of European silver eels, our present results show, at the opposite, an impairment of the transcription level of genes involved in the mitochondrial respiratory chain.…”

Section: Discussionmentioning

confidence: 84%

“…Whereas a significant and progressive accumulation of mts mRNA was observed from pre-vitellogenic to vitellogenic oocytes and eggs, no significant accumulation of MTs proteins could be observed. Analogously, whereas SAGE analysis carried out on zebrafish ovaries revealed high levels of mt2 transcripts, the concomitant proteomic analysis failed to reveal significant amounts of the corresponding proteins (Knoll-Gellida et al 2006). As already evoked, such mRNA accumulation could aim to provide sufficient materials needed for embryogenesis.…”

Section: Discussionmentioning

confidence: 92%

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Ovarian gene transcription and effect of cadmium pre-exposure during artificial sexual maturation of the European eel (Anguilla anguilla)

Pierron

Baudrimont

Dufour³

et al. 2009

Biometals

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European eels are dangerously threatened with extinction. Recent advances tend to show that pollution could, in addition to other already identified factors, contribute to this drama. In a previous report, cadmium (Cd) pre-exposure was found to strongly stimulate the pituitary-liver-gonad axis of maturing female silver eels, leading, lastly, to oocytes atresia and eels mortality. The present work was performed to get more insights into the effects of Cd preexposure on eels' ovaries. The transcription levels of various genes involved in mitochondrial metabolism, in the cellular response to metal (metallothioneins, MTs) and oxidative stress (catalase, CAT) were investigated. Our results show that ovarian growth is associated with an up-regulation of mitochondrial genes. However, Cd pre-exposure was found to significantly impair this up-regulation. Such findings could explain, at least in part, why oocytes of Cd pre-contaminated eels could not reach final maturation. Concerning MTs, despite the end of the experiment was marked by a strong increase in their gene transcription levels in both eel groups, MTs protein content was found to increase only in the case of Cd pre-contaminated eels. Since this increase in MTs protein content was associated with a massive entry of Cd in gonads, our findings suggest that MTs mRNA, that are normally accumulated in oocytes to cope with the future needs, can be activated and translated in response to Cd exposure.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 92%

Ovarian gene transcription and effect of cadmium pre-exposure during artificial sexual maturation of the European eel (Anguilla anguilla)

Pierron

Baudrimont

Dufour³

et al. 2009

Biometals

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“…By comparing the information available in the expression libraries for oocytes/unfertilized eggs from diverse vertebrates, including fish, frog, pig, mouse and human, several of these highly expressed transcripts were identified to be a part of a commonly expressed vertebrate ovarian gene signature (Knoll-Gellida et al 2006). However, it has to be noted that while some of these genes, such as those coding for diverse zona pellucida proteins, are clearly female-related, the signature specificity for some other abundant transcripts is questionable, as they may be highly expressed in several tissue types, being involved in general cellular functions.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have examined gene expression in zebrafish testis and ovary on the mRNA level (Knoll-Gellida et al 2006; Santos et al 2007; Jorgensen et al 2008; Sreenivasan et al 2008; Li et al 2004; Zeng and Gong 2002; Wen et al 2005). In mature zebrafish ovarian follicles, the transcript abundance measured by serial analysis of gene expression provided little predictive value with respect to the extent of protein abundance, measured with 2D-GE (Knoll-Gellida et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio)

Groh

Nesatyy

Segner

et al. 2011

Fish Physiol Biochem

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The molecular mechanisms controlling sex determination and differentiation in zebrafish (Danio rerio) are largely unknown. A genome-wide analysis may provide comprehensive insights into the processes involved. The mRNA expression in zebrafish gonads has been fairly well studied, but much less data on the corresponding protein expression are available, although the proteins are considered to be more relevant markers of gene function. Because mRNA and protein abundances rarely correlate well, mRNA profiles need to be complemented with the information on protein expression. The work presented here analyzed the proteomes of adult zebrafish gonads by a multidimensional protein identification technology, generating the to-date most populated lists of proteins expressed in mature zebrafish gonads. The acquired proteomics data partially confirmed existing transcriptomics information for several genes, including several novel transcripts. However, disagreements between mRNA and protein abundances were often observed, further stressing the necessity to assess the expression on different levels before drawing conclusions on a certain gene’s expression and function. Several gene groups expressed in a sexually dimorphic way in zebrafish gonads were identified. Their potential importance for gonad development and function is discussed. The data gained in the current study provide a basis for further work on elucidating processes occurring during zebrafish development with use of high-throughput proteomics.Electronic supplementary materialThe online version of this article (doi:10.1007/s10695-010-9464-x) contains supplementary material, which is available to authorized users.

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“…However, large scale toxicoproteomics studies in the fish ovary have been limited. Knoll-Gellida et al (2006) investigated the protein complement of fully grown zebrafish follicles and identified a number of proteins using 1D-SDS-PAGE and 2D-PAGE analysis. The proteins identified included zona pellucida glycoproteins and Vtg isoforms (Vtg1 and Vtg).…”

Section: Applying Proteomics To Study the Fish Ovarian Proteomementioning

confidence: 99%

DIGE and iTRAQ as biomarker discovery tools in aquatic toxicology

Martyniuk

Alvarez

Denslow

2012

Ecotoxicology and Environmental Safety

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Molecular approaches in ecotoxicology have greatly enhanced mechanistic understanding of the impact of aquatic pollutants in organisms. These methods+- have included high throughput Omics technologies, including quantitative proteomics methods such as 2D differential in-gel electrophoresis (DIGE) and isobaric tagging for relative and absolute quantitation (iTRAQ). These methods are becoming more widely used in ecotoxicology studies to identify and characterize protein bioindicators of adverse effects. In teleost fish, iTRAQ has been used successfully in different fish species (e.g. fathead minnow, goldfish, largemouth bass) and tissues (e.g. hypothalamus and liver) to quantify relative protein abundance. Of interest for ecotoxicology is that many proteins commonly utilized as bioindicators of toxicity or stress are quantifiable using iTRAQ on a larger scale, providing a global baseline of biological effect from which to assess additional changes in the proteome. This review highlights the successes to date for high throughput quantitative proteomics using DIGE and iTRAQ in aquatic toxicology. Current challenges for the iTRAQ method for biomarker discovery in fish are the high cost and the lack of complete annotated genomes for teleosts. However, the use of protein homology from teleost fishes in protein databases and the introduction of hybrid LTQ-FT (Linear ion trap –Fourier transform) mass spectrometers with high resolution, increased sensitivity, and high mass accuracy are able to improve significantly the protein identification rates. Despite these challenges, initial studies utilizing iTRAQ for ecotoxicoproteomics have exceeded expectations and it is anticipated that the use of non-gel based quantitative proteomics will increase for protein biomarker discovery and for characterization of chemical mode of action.

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Molecular phenotype of zebrafish ovarian follicle by serial analysis of gene expression and proteomic profiling, and comparison with the transcriptomes of other animals

Cited by 84 publications

References 93 publications

Ovarian gene transcription and effect of cadmium pre-exposure during artificial sexual maturation of the European eel (Anguilla anguilla)

Ovarian gene transcription and effect of cadmium pre-exposure during artificial sexual maturation of the European eel (Anguilla anguilla)

Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio)

DIGE and iTRAQ as biomarker discovery tools in aquatic toxicology

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