Molecular phylogenetic analyses reveal a deep dichotomy in the conifer-inhabiting genus Trisetacus (Eriophyoidea: Nalepellidae), with the two lineages differing in their female genital morphology and host associations

On some morphological and ultrastructural features of the insemination system in five species of the genus Brevipalpus (Acari: Tenuipalpidae)

“…The shape of the spermatheca has already been used for discerning mite species in Phytoseiidae (e.g., Chant and McMurtry 2007 ). Moreover, attempts have been made to use the morphology of this structure to separate species in some plant mites (e.g., Vacante 1983 , 1984 ; Beard et al 2015 ) and this structure morphology could also be correlated to molecular phylogeny lineages suggesting it is evolutionarily conserved in groups like Eriophyoidea (Chetverikov et al 2015 , 2020 ; Chetverikov and Petanović 2016 ).…”

Phylogenetic Position of a New Trisetacus Mite Species (Nalepellidae) Destroying Seeds of North American Junipers and New Hypotheses on Basal Divergence of Eriophyoidea

“…Median line appears concave, projecting from posterior margin of prodorsal shield up to the base of tubercle vi; in some females median line can be fragmented, very faint and almost imperceptible. Admedian lines absent like in other Trisetacus spp., associated with Cupressaceae [23,30]. Submedian I short, ridge-like, can be broken and fragmented; right and left submedian I converge postero-medially forming in most studied mites a figure resembling a flattened U.…”

“…Sequence alignment and molecular phylogenetic analyses. A large set of closely related and distant eriophyoid out-groups sequenced in previous studies were included in the analysis: complete dataset of Nalepellidae [23], all Cox1 gene sequences of Eriophyidae s.l. from Genbank that are longer than 1100 bp, ten sequences of phytoptids, and three sequences of pentasetacids.…”

“…Eriophyoid mites of the genus Trisetacus Keifer (Acariformes, Eriophyoidea, Nalepellidae) are common and widespread arthropod parasites of conifers [21,22]. They have been recorded in all regions where conifers are abundant except Australasia, where no studies on eriophyoids from gymnosperms have been performed to date [23]. All known hosts of Trisetacus belong to the two most diverse gymnosperm families, Pinaceae and Cupressaceae.…”

“…During field surveys in mountainous areas of the western USA we observed groves of junipers heavily infested by a new Trisetacus species. In this paper we aim to describe this species and assess its phylogenetic position within the genus Trisetacus, based on sequence comparisons of the mitochondrial cytochrome oxidase c gene (Cox1), including a dataset from a previous study [23]. In addition, we investigated the phylogenetic structure of Eriophyoidea using a new set of outgroups and a translated Cox1 amino-acid alignment that was twice as long as that produced by the classical LCO-HCO fragment [31].…”