Molecular Phylogenetic Analysis of Ribosomal DNA Internal Transcribed Spacer Regions and Comparison of Fertility in Phomopsis Isolates from Fruit Trees

Kanematsu, Satoko; Minaka, Nobuhiro; Kobayashi, Takao; Kudo, Akira; Ohtsu, Yoshihiro

doi:10.1007/pl00012944

Cited by 26 publications

(20 citation statements)

References 30 publications

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“…However, they did not notice any morphological variability or differences in virulence and pathogenicity within the two groups. The weak pathogenic D. eres has been widely reported associated with ericaceous, rosaceous fruit trees and grapevines from Asia, Europe and USA (Kanematsu et al 1999(Kanematsu et al , 2000(Kanematsu et al , 2007Kaliterna et al 2012;Lombard et al 2014). Additionally Phomopsis sp.…”

Section: Discussionmentioning

confidence: 99%

Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex

et al. 2014

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The genus Diaporthe comprises pathogenic, endophytic and saprobic species with both temperate and tropical distributions. Cryptic diversification, phenotypic plasticity and extensive host associations have long complicated accurate identifications of species in this genus. The delimitation of the generic type species Diaporthe eres has been uncertain due to the lack of ex-type cultures. Species limits of D. eres and closely related species were evaluated using molecular phylogenetic analysis of eight genes including nuclear ribosomal internal transcribed spacer (ITS), partial sequences of actin (ACT), DNA-lyase (Apn2), translation elongation factor 1-α (EF1-α), beta-tubulin (TUB), calmodulin (CAL), 60s ribosomal protein L37 (FG1093) and histone-3 (HIS). The occurrence of sequence heterogeneity of ITS within D. eres is observed, which complicates the analysis and may lead to overestimation of the species diversity. The strict criteria of Genealogical Concordance Phylogenetic Species Recognition (GCPSR) were applied to resolve species boundaries based on individual and combined analyses of other seven genes except the ITS. We accept nine distinct phylogenetic species including helicis and D. pulla. Modern descriptions and illustrations are provided for these species. Newly designed primers are introduced to amplify and sequence the Apn2 (DNA-lyase) gene in Diaporthe. Based on phylogenetic informativeness profiles, EF1-α, Apn2 and HIS genes are recognised as the best markers for defining species in the D. eres complex.

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Section: Discussionmentioning

confidence: 99%

Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex

et al. 2014

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“…An Applied Biosystems GeneAmp 9700 thermal cycler was used to amplify the rDNA internal transcribed spacer (ITS) region using primer pairs ITS1 and ITS4 ). PCR conditions and sequence analysis followed the method of Kanematsu et al (2000). Sequence data were aligned with other Ceratocystis species using the program FSA version 1.15.2 (Bradley et al 2009).…”

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confidence: 99%

Ceratocystis ficicola sp. nov., a causal fungus of fig canker in Japan

Kajitani

Masuya

2011

Mycoscience

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The causal fungus of Ceratocystis canker of fig in Japan is described as Ceratocystis ficicola sp. nov. This species is characterized by galeated ascospores and is similar to Ceratocystis fimbriata sensu lato. However, the perithecia of the new species are much larger than those of C. fimbriata. The fungus grows more rapidly than C. fimbriata sensu stricto at 25°C but more slowly at 30°C. Molecular analysis of the nucleotide sequences of rDNA ITS regions showed that C. ficicola is phylogenetically placed in the clade of C. fimbriata s. l. but is easily distinguishable from other species of C. fimbriata s. l.

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“…J.J. Tuset & M.T. Portilla, cause of peach and almond canker (Farr et al 1999;Kanematsu et al 1999Kanematsu et al , 2000, although this species has recently been isolated from grape (van Niekerk et al 2005). Other recently described, hostspecifi c species of Phomopsis include P. gossypii (Sacc.)…”

Section: Diaporthaceaementioning

confidence: 99%